نشریه دامپزشکی ایران

نشریه دامپزشکی ایران

اثر پروبیوتیک‌های ضد درک حد نصاب بر تعدیل فعالیت آنزیم‌های گوارشی، فلور میکروبی، عملکرد رشد و پارامترهای بیوشیمیایی در ماهی کپور معمولی (Cyprinus carpio)

نوع مقاله : مقاله پژوهشی

نویسندگان
1 دانش‌آموخته دکتری تخصصی بهداشت آبزیان، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران
2 دانشیار گروه بهداشت دام، طیور و آبزیان، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران و عضو قطب بهداشت و بیماری‌های ماهیان گرمابی دانشگاه شهید چمران اهواز، اهواز، ایران
3 استاد گروه بهداشت دام، طیور و آبزیان، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران و عضو قطب بهداشت و بیماری‌های ماهیان گرمابی دانشگاه شهید چمران اهواز، اهواز، ایران
4 استاد گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران و عضو قطب بهداشت و بیماری‌های ماهیان گرمابی دانشگاه شهید چمران اهواز، اهواز، ایران
5 دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران
چکیده
    در مطالعه حاضر، دو پروبیوتیک اصلی (Citrobacter freundii و Bacillus foraminis) با ویژگی ضد درک حد نصاب  از روده ماهی کپور معمولی جداسازی شدند و اثرات آن­ها بر عملکرد رشد، فلور میکروبی روده، شاخص‌های بیوشیمیایی و فعالیت آنزیم‌های گوارشی، مانند الفا آمیلاز، لیپاز، تریپسین، کیموتریپسین، و آلکالین فسفاتاز در ماهی کپور معمولی مورد بررسی قرار گرفت.  بچه ماهیان (تعداد 450 قطعه با وزن 10±50 گرم) به طور تصادفی به 6 گروه مساوی (با 3 تکرار) تقسیم شدند و با جیره­ های حاوی QQ1 (C. freundii به میزان 1×109 CFU/g)، QQ2 (B. foraminis به میزان 1×109 CFU/g)، WQQ (L. plantarum باکتری فاقد ویژگی ضد درک حد نصاب) به میزان 1×109 CFU/g)، QQ2+ QQ1 (B. foraminis و C. freundii به میزان مساوی 1×109 CFU/g)، گروه ترکیبی (B. foraminis و C. freundii و L. plantarum به میزان مساوی 1×109 CFU/g) و یک رژیم غذایی کنترل (بدون پروبیوتیک) برای 60 روز به طور مداوم تغذیه شدند.  نتایج نشان داد که مکمل­ های پروبیوتیک به طور کلی اثرات معنی­ داری بر عملکرد رشد داشتند.  گروه­ های ترکیبی و پلانتاروم به ترتیب در روزهای 30 و 60 بهترین اثر را بر نرخ رشد ویژه (SGR) و راندمان مصرف خوراک در کپور معمولی داشتند.  فعالیت­ های تریپسین، پروتئاز و کیموتریپسین در روز 30 پس از تغذیه، در تیمار ترکیبی در مقایسه با گروه شاهد و سایر گروه ­ها به طور معنی­ داری افزایش یافت.  تغییرات معنی ­داری در فلور باکتریایی روده در تمامی گروه­ های پروبیوتیک نسبت به گروه شاهد مشاهده شد.  این نتایج استفاده بالقوه از QQ2 (B. foraminis) را به تنهایی یا در ترکیب سایر پروبیوتیک­ ها (تیمار ترکیبی) به عنوان افزودنی در رژیم غذایی کپور معمولی نشان داد اما در طولانی مدت توصیه نمی­ شود.  نتایج نشان داد که مکمل‌سازی باکتری‌های جدا شده از روده کپور معمولی (مانند لاکتوپلانتی باسیلوس پلانتاروم) می‌تواند عملکرد رشد، میکروبیوتای روده و برخی فعالیت‌های آنزیم گوارشی را در بچه ماهیان کپور معمولی در دورهای پروروش طولانی‌مدت بهبود بخشد.  بنابراین، می ­توان آن را مانند پروبیوتیک ­های تجاری به عنوان تقویت کننده رشد استفاده نمود.
کلیدواژه‌ها

موضوعات


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