نشریه دامپزشکی ایران

نشریه دامپزشکی ایران

تأثیر میکروپلاستیک پلی‌استایرن بر پارامترهای هیستومورفومتریک بیضه در موش نر بالغ

نوع مقاله : مقاله پژوهشی

نویسندگان
1 دانشجوى دکتراى تخصصى بافت‌شناسی مقایسه‌ای، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران
2 استاد گروه علوم پایه، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران
3 دانش‌آموخته دکتراى تخصصى بافت‌شناسی مقایسه‌ای، گروه علوم پایه، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران
چکیده
    میکروپلاستیک‌ها به عنوان یک آلاینده جدید محیطی پایدار، می‌توانند از موانع تغذیه­ ای عبور و در بافت­ های مختلف بدن تجمع کنند.  با این حال، اثرات بافت­ شناسی و هیستومورفومتری میکروپلاستیک پلی­استایرن بر بافت بیضه و مکانیسم آن‌ها نامشخص است.  مطالعه حاضر به منظور ارزیابی اثرات میکروپلاستیک پلی­استایرن بر پارامترهای هیستومورفومتریک بافت بیضه در موش انجام گرفت.  در این مطالعه، 36 سر موش نر بالغ نژاد NMRI به چهار گروه 9 تایی تقسیم شدند.  سه گروه از موش ­ها میکروپلاستیک پلی­ استایرن را با دوزهای 01/0، 1/0 و 1 میلی‌گرم بر کیلوگرم وزن بدن از طریق گاواژ به مدت 42 روز دریافت کردند.  گروه کنترل نیز 1 میلی­ لیتر آب مقطر را به صورت گاواژ دریافت کرد.  نمونه­ های بافتی بیضه 24 ساعت پس از آخرین درمان از موش‌ها جمع ­آوری و جهت بررسی ­های هیستومورفومتریک مورد استفاده قرار گرفت.  مطالعه حاضر نشان داد که پلی ­استایرن موجب کاهش معنی­ داری در پارامترهای ارتفاع اپیتلیوم ژرمینال، قطر لوله ­های اسپرم‌ساز، تعداد سلول­ های سرتولی، تعداد سلول‌های لیدیگ، تعداد اسپرماتوسیت­ ها، ضریب تمایز لوله‌ای، ضریب اسپرمیوژنز ،ضریب تجمعی، ضریب میوزی و شاخص جانسن نسبت به گروه کنترل گردید و همچنین پلی­ استایرن موجب شد پارامترهای ضخامت کپسول بیضه، تعداد سلول­ های ایمنی تک­ هسته ­ای و ضخامت بافت همبند بینابینی نسبت به گروه کنترل به طور معنی ­داری افزایش یابد.  یافته‌های ما نشان داد که تجویز میکروپلاستیک پلی ­استایرن موجب تغییرات گسترده در اندازه و ساختار بافتی بیضه در موش‌ها می ­شود که بر سیستم تولیدمثلی تاثیرگذار است و در نهایت موجب اثرات منفی بر توان باروری در موش می گردد.
کلیدواژه‌ها

موضوعات


An, R., Wang, X., Yang, L., Zhang, J., Wang, N., Xu, F., Hou, Y., Zhang, H., & Zhang, L. (2021). Polystyrene microplastics cause granulosa cells apoptosis and fibrosis in ovary through oxidative stress in rats. Toxicology449, 152665.
Anbara, H., Shahrooz, R., Razi, M., Malekinejad, H., & Najafi, G. (2018). The effect of vitamin C on mice hemolytic anemia induced by phenylhydrazine: an animal model study using histological changes in testis, pre-implantation embryo development, and biochemical changes. Iranian journal of basic medical sciences21(7), 668–677.
Anbara, H., Sheibani, M. T., & Razi, M. (2020). Long-Term Effect of Aspartame on Male Reproductive System: Evidence for Testicular Histomorphometrics, Hsp70-2 Protein Expression and Biochemical Status. International journal of fertility & sterility14(2), 91-101.
Anbara, H., Sheibani, M. T., Razi, M., & Kian, M. (2021). Insight into the mechanism of aspartame-induced toxicity in male reproductive system following long-term consumption in mice model. Environmental toxicology36(2), 223-237.
Andrady A. L. (2017). The plastic in microplastics: A review. Marine pollution bulletin119(1), 12-22.
Bouwmeester, H., Hollman, P. C., & Peters, R. J. (2015). Potential Health Impact of Environmentally Released Micro- and Nanoplastics in the Human Food Production Chain: Experiences from Nanotoxicology. Environmental science & technology49(15), 8932-8947.
Chamas, A., Moon, H., Zheng, J., Qiu, Y., Tabassum, T., Jang, J. H., Abu-Omar, M., Scott, S. L., & Suh, S. (2020). Degradation Rates of Plastics in the Environment. ACS Sustainable Chemistry and Engineering, 8(9), 3494-3511.
De Gendt, K., Swinnen, J. V., Saunders, P. T., Schoonjans, L., Dewerchin, M., Devos, A., Tan, K., Atanassova, N., Claessens, F., Lécureuil, C., Heyns, W., Carmeliet, P., Guillou, F., Sharpe, R. M., & Verhoeven, G. (2004). A Sertoli cell-selective knockout of the androgen receptor causes spermatogenic arrest in meiosis. Proceedings of the National Academy of Sciences of the United States of America101(5), 1327-1332.
Deng, Y., Yan, Z., Shen, R., Huang, Y., Ren, H., & Zhang, Y. (2021). Enhanced reproductive toxicities induced by phthalates contaminated microplastics in male mice (Mus musculus). Journal of hazardous materials406, 124644.
Duis, K., & Coors, A. (2016). Microplastics in the aquatic and terrestrial environment: sources (with a specific focus on personal care products), fate and effects. Environmental sciences Europe28(1), 2.
Hai, Y., Hou, J., Liu, Y., Liu, Y., Yang, H., Li, Z., & He, Z. (2014). The roles and regulation of Sertoli cells in fate determinations of spermatogonial stem cells and spermatogenesis. Seminars in cell & developmental biology29, 66-75.
Hale, R. C., Seeley, M. E., La Guardia, M. J., Mai, L., & Zeng, E. Y. (2020). A Global Perspective on Microplastics. In Journal of Geophysical Research: Oceans, 125, e2018JC014719.
Hariharan, G., Purvaja, R., Anandavelu, I., Robin, R. S., & Ramesh, R. (2021). Accumulation and ecotoxicological risk of weathered polyethylene (wPE) microplastics on green mussel (Perna viridis). Ecotoxicology and environmental safety208, 111765.
He, J., Yang, X., & Liu, H. (2021). Enhanced toxicity of triphenyl phosphate to zebrafish in the presence of micro- and nano-plastics. The Science of the total environment756, 143986.
Hernandez, L. M., Xu, E. G., Larsson, H. C. E., Tahara, R., Maisuria, V. B., & Tufenkji, N. (2019). Plastic Teabags Release Billions of Microparticles and Nanoparticles into Tea. Environmental science & technology53(21), 12300-12310.
Hou, B., Wang, F., Liu, T., & Wang, Z. (2021). Reproductive toxicity of polystyrene microplastics: In vivo experimental study on testicular toxicity in mice. Journal of hazardous materials405, 124028.
Jin, H., Ma, T., Sha, X., Liu, Z., Zhou, Y., Meng, X., Chen, Y., Han, X., & Ding, J. (2021a). Polystyrene microplastics induced male reproductive toxicity in mice. Journal of hazardous materials401, 123430.
Jin, H., Yan, M., Pan, C., Liu, Z., Sha, X., Jiang, C., & Ding, J. (2021b). Reproductive Toxicity of Chronic Exposure To Polystyrene Microplastics And The Molecular Mechanism of Decrease In Testosterone Levels In Male Mice. Research Square. https://doi.org/10.21203/rs.3.rs-689230/v1
Jin, H., Yan, M., Pan, C., Liu, Z., Sha, X., Jiang, C., Li, L., Pan, M., Li, D., Han, X., & Ding, J. (2022). Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway. Particle and fibre toxicology19(1), 13.
Jin, Y., Lu, L., Tu, W., Luo, T., & Fu, Z. (2019). Impacts of polystyrene microplastic on the gut barrier, microbiota and metabolism of mice. The Science of the total environment649, 308-317.
Kim, S. W., & An, Y. J. (2020). Edible size of polyethylene microplastics and their effects on springtail behavior. Environmental pollution266(1), 115255.
Krzastek, S. C., Farhi, J., Gray, M., & Smith, R. P. (2020). Impact of environmental toxin exposure on male fertility potential. Translational andrology and urology9(6), 2797–2813.
Li, S., Wang, Q., Yu, H., Yang, L., Sun, Y., Xu, N., Wang, N., Lei, Z., Hou, J., Jin, Y., Zhang, H., Li, L., Xu, F., & Zhang, L. (2021). Polystyrene microplastics induce blood-testis barrier disruption regulated by the MAPK-Nrf2 signaling pathway in rats. Environmental science and pollution research international28(35), 47921–47931.
Liu, Z., Qin, Q., Hu, Z., Yan, L., Ieong, U. I., & Xu, Y. (2020). Adsorption of chlorophenols on polyethylene terephthalate microplastics from aqueous environments: Kinetics, mechanisms and influencing factors. Environmental pollution (Barking, Essex : 1987)265(Pt A), 114926.
Lu, L., Wan, Z., Luo, T., Fu, Z., & Jin, Y. (2018). Polystyrene microplastics induce gut microbiota dysbiosis and hepatic lipid metabolism disorder in mice. The Science of the total environment631-632, 449-458.
Marcelino, R. C., Cardoso, R. M., Domingues, E. L. B. C., Gonçalves, R. V., Lima, G. D. A., & Novaes, R. D. (2022). The emerging risk of microplastics and nanoplastics on the microstructure and function of reproductive organs in mammals: A systematic review of preclinical evidence. Life sciences295, 120404.
Mousavi, A., Gharzi, A., Gholami, M., Beyranvand, F., & Takesh, M. (2021). The therapeutic effect of cerium oxide nanoparticle on ischaemia/reperfusion injury in rat testis. Andrologia53(11), e14231.
Napper, I. E., & Thompson, R. C. (2020). Plastic Debris in the Marine Environment: History and Future Challenges. Global challenges4(6), 1900081.
Soltani M, Ghotbeddin Z, Rahimi K, Tabandeh MR. (2024). Effects of Alpha-pinene on oxidative stress and inflammatory response in acute gastric ulcers in rats. Iranian Veterinary Journal, 20(3):101-13.
Soltani, N. S., Taylor, M. P., & Wilson, S. P. (2022). International quantification of microplastics in indoor dust: prevalence, exposure and risk assessment.  Environmental pollution312, 119957.
Nikolic, S., Gazdic-Jankovic, M., Rosic, G., Miletic-Kovacevic, M., Jovicic, N., Nestorovic, N., Stojkovic, P., Filipovic, N., Milosevic-Djordjevic, O., Selakovic, D., Zivanovic, M., Seklic, D., Milivojević, N., Markovic, A., Seist, R., Vasilijic, S., Stankovic, K. M., Stojkovic, M., & Ljujic, B. (2022). Orally administered fluorescent nanosized polystyrene particles affect cell viability, hormonal and inflammatory profile, and behavior in treated mice. Environmental pollution305, 119206.
Pitt, J. A., Trevisan, R., Massarsky, A., Kozal, J. S., Levin, E. D., & Di Giulio, R. T. (2018). Maternal transfer of nanoplastics to offspring in zebrafish (Danio rerio): A case study with nanopolystyrene. The Science of the total environment643, 324-334.
Pirzadeh A, Morovvati H, Abbasi M. (2024). Study on the effect of Spirulina platensis on the histomorphometric and histological structure of rat testicular tissue exposed to iron oxide nanoparticles. Iranian Veterinary Journal, 20(2):16-26.
Preston-Whyte, F., Silburn, B., Meakins, B., Bakir, A., Pillay, K., Worship, M., Paruk, S., Mdazuka, Y., Mooi, G., Harmer, R., Doran, D., Tooley, F., & Maes, T. (2021). Meso- and microplastics monitoring in harbour environments: A case study for the Port of Durban, South Africa. Marine pollution bulletin163, 111948.
Qiang, L., Lo, L. S. H., Gao, Y., & Cheng, J. (2020). Parental exposure to polystyrene microplastics at environmentally relevant concentrations has negligible transgenerational effects on zebrafish (Danio rerio). Ecotoxicology and environmental safety206, 111382.
Schulz, R. W., França, L. R. de, Lareyre, J. J., Le Gac, F., Chiarini-Garcia, H., Nobrega, R. H., & Miura, T. (2010). Erratum to Spermatogenesis in fish [Gen. Comp. Endocrinol. 165, (2010), 390-411]. In General and Comparative Endocrinology, 167(1), 179.
Schwabl, P., Köppel, S., Königshofer, P., Bucsics, T., Trauner, M., Reiberger, T., & Liebmann, B. (2019). Detection of Various Microplastics in Human Stool: A Prospective Case Series. Annals of internal medicine171(7), 453-457.
Wang, J., Li, Y., Lu, L., Zheng, M., Zhang, X., Tian, H., Wang, W., & Ru, S. (2019). Polystyrene microplastics cause tissue damages, sex-specific reproductive disruption and transgenerational effects in marine medaka (Oryzias melastigma). Environmental pollution254(Pt B), 113024.
Wang, S., Han, Q., Wei, Z., Wang, Y., Xie, J., & Chen, M. (2022a). Polystyrene microplastics affect learning and memory in mice by inducing oxidative stress and decreasing the level of acetylcholine. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association162, 112904.
Wang, X., Zhang, X., Sun, K., Wang, S., & Gong, D. (2022b). Polystyrene microplastics induce apoptosis and necroptosis in swine testis cells via ROS/MAPK/HIF1α pathway. Environmental toxicology37(10), 2483-2492.
Wei, W., Chen, X., Peng, L., Liu, Y., Bao, T., & Ni, B. J. (2021a). The entering of polyethylene terephthalate microplastics into biological wastewater treatment system affects aerobic sludge digestion differently from their direct entering into sludge treatment system. Water research190, 116731.
Wei, Y., Zhou, Y., Long, C., Wu, H., Hong, Y., Fu, Y., Wang, J., Wu, Y., Shen, L., & Wei, G. (2021b). Polystyrene microplastics disrupt the blood-testis barrier integrity through ROS-Mediated imbalance of mTORC1 and mTORC2. Environmental pollution289, 117904.
Wei, Z., Wang, Y., Wang, S., Xie, J., Han, Q., & Chen, M. (2022). Comparing the effects of polystyrene microplastics exposure on reproduction and fertility in male and female mice. Toxicology465, 153059.
Wu, B., Wu, X., Liu, S., Wang, Z., & Chen, L. (2019). Size-dependent effects of polystyrene microplastics on cytotoxicity and efflux pump inhibition in human Caco-2 cells. Chemosphere221, 333-341.
Xie, X., Deng, T., Duan, J., Xie, J., Yuan, J., & Chen, M. (2020). Exposure to polystyrene microplastics causes reproductive toxicity through oxidative stress and activation of the p38 MAPK signaling pathway. Ecotoxicology and environmental safety190, 110133.
Zarus, G. M., Muianga, C., Hunter, C. M., & Pappas, R. S. (2021). A review of data for quantifying human exposures to micro and nanoplastics and potential health risks. The Science of the total environment756, 144010.
Zhang, C., Wang, J., Zhou, A., Ye, Q., Feng, Y., Wang, Z., Wang, S., Xu, G., & Zou, J. (2021). Species-specific effect of microplastics on fish embryos and observation of toxicity kinetics in larvae. Journal of hazardous materials403, 123948.
Zhang, D. M., Guo, Z. X., Zhao, Y. L., Wang, Q. J., Gao, Y. S., Yu, T., Chen, Y. K., Chen, X. M., & Wang, G. Q. (2019). L-carnitine regulated Nrf2/Keap1 activation in vitro and in vivo and protected oxidized fish oil-induced inflammation response by inhibiting the NF-κB signaling pathway in Rhynchocypris lagowski Dybowski. Fish & shellfish immunology93, 1100-1110.
Zhou, R., Wu, J., Liu, B., Jiang, Y., Chen, W., Li, J., He, Q., & He, Z. (2019). The roles and mechanisms of Leydig cells and myoid cells in regulating spermatogenesis. Cellular and molecular life sciences, 76(14), 2681-2695.