Cloning and expression of VP28 gene of white spot virus (Iran isolate) in E. coli, TG1

Houshmand, Hossein; Seyfi Abad Shapouri, Masoud Reza; Peyghan, Rahim; G.Marammazi, Jasem; Afsharnasab, Mohammad

doi:10.22055/ivj.2015.8717

Cloning and expression of VP28 gene of white spot virus (Iran isolate) in E. coli, TG1

Document Type : Research Paper

Authors

Hossein Houshmand ¹

Masoud reza seyfi abad shapouri ²

Rahim Peyghan ³

Jasem G.Marammazi ⁴

Mohammad Afsharnasab ⁵

¹ Head of Aquatic Animals Health and Diseases Dept. South of Iran Aquaculture Research Center

² Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz

³ Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz

⁴ Associate Professor,Iranian Fisheries Reseach Organization, South of Iran Aquaculture Reseach Center, Ahvaz

⁵ Associate Professor,Iranian Fisheries Reseach Organization, Tehran,Iran.

10.22055/ivj.2015.8717

Abstract

White spot virus is concerned as a major pathogen in shrimp Penaeids family. In recent years, heavy mortalities in cultured shrimps Iran and other countries necessitate doing studies to construct kits for rapid detection and eliciting resistance against the virus by using different membrane proteins. For this purpose, in this study, a piece of VP28 gene of white spot virus with 528 bp length was isolated and amplified by PCR from the infected shrimps in Choebdeh Abadan. After cloning of the gene in the plasmid pMal-c2X, the protein was expressed in E. coli strain TG1. Target protein with a molecular weight of 19.38 kDa was evaluated by SDS-PAGE. Expression of protein with the expected molecular weight in SDS-PAGE accompanied with evaluation of the cloned sequences revealed the successful expression of the protein in Escherichia coli TG1. This study for the first time succeeded in cloning and experssion of VP28 in a prokaryotic organism.

Keywords

white spot virus

VP28

Cloning

Expression

Subjects