Clostridium perfringens type ِD strains cause severe enteroxemia in sheep, calves, goats, pigs and chickens. epsilon-toxin is introduced to be the essential virulence factor of this microorganism. In the present study, a new method was established for the purification of epsilon toxin from culture supernatant fluid of Clostridium perfringens type D vaccinal strain. The three steps of the purification scheme involved ammonium sulfate precipitation, cation exchange chromatography (CM- Sepharose) and gel filtration (Sephadex G-100). epsilon toxin was purified about 138.9-fold from the Sephadex G-100 column in terms of the toxin lethality. The molecular weight of the epsilon toxin determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was approximately 34kD. MDCK exposed to epsilon toxin showed a cell border retraction, cytoplasmic blebbing, cell shrinkage and cell rounding. In conclusion, this new method for purification of epsilon toxin of clostridium perfringens type D is reliable and the purified epsilon toxin has high activity.
zayerzadeh, E. & Fardipour, A. (2015). purification of epsilon toxin from vaccinal strain of clostridium perfringens type D using ion exchange chromatography and gel filtration. Iranian Veterinary Journal, 11(1), 92–102. https://doi.org/10.22055/ivj.2015.8715
MLA
zayerzadeh, E., & Fardipour, A. "purification of epsilon toxin from vaccinal strain of clostridium perfringens type D using ion exchange chromatography and gel filtration", Iranian Veterinary Journal, 11, 1, 2015, 92–102. doi: 10.22055/ivj.2015.8715
HARVARD
zayerzadeh, E., & Fardipour, A. (2015). 'purification of epsilon toxin from vaccinal strain of clostridium perfringens type D using ion exchange chromatography and gel filtration', Iranian Veterinary Journal, 11(1), pp. 92–102. doi: 10.22055/ivj.2015.8715
CHICAGO
zayerzadeh, E. & Fardipour, A., "purification of epsilon toxin from vaccinal strain of clostridium perfringens type D using ion exchange chromatography and gel filtration." Iranian Veterinary Journal, 11 1 (2015): 92–102, doi: 10.22055/ivj.2015.8715
VANCOUVER
zayerzadeh E., Fardipour A. purification of epsilon toxin from vaccinal strain of clostridium perfringens type D using ion exchange chromatography and gel filtration. Iran Vet J. 2015;11(1):92–102 (In Persian). doi: 10.22055/ivj.2015.8715
