Study of polymorphism in intron 4 and exon 5 of Ghrelin gene in Khouzestan native chickens using PCR-RFLP

Rezaei Yazd Abadi, S; Roshanfekr, H; Beigi Nasiri, M.T.; Fayazi, J

doi:10.22055/ivj.2017.43262.1632

Study of polymorphism in intron 4 and exon 5 of Ghrelin gene in Khouzestan native chickens using PCR-RFLP

Document Type : Research Paper

Authors

S Rezaei Yazd Abadi ¹

H Roshanfekr ²

M.T. Beigi Nasiri ³

J Fayazi ²

¹ MSc Gradueted of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran

² Associate Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran

³ Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran

10.22055/ivj.2017.43262.1632

Abstract

Abstract

This study was performed in order to identify polymorphism in fourth intron and fifth exon in Khuzestan province native chicken’s ghrelin gene using PCR-RFLP method. Therefore, blood sampling from 100 chickens of Native chickens in Khuzestan province breeding and Jihad livestock inputs randomly was performed. DNA extraction from blood samples was performed using salt method. The quantity and quality of DNA was determined using gel electrophoresis (1%) and Nanodrop respectively and then 228 bp fragment of fourth intron and fifth exon region was amplified using polymerase chain reaction (PCR). Eventually amplified samples were digested with HaeIII enzyme and digest parts of 173 and 55 bp were found. The results showed that in all the samples after enzymatic digestion was only a same band shape therefore, considering the same genotype samples examined in this study, mutation was not founded. Not found polymorphism may be due to closed nurture environment and the low number of samples.

Keywords

Khouzestan native chickens

Ghrelin gene

PCR-RFLP