This experiment was conducted to investigate the effects of different concentrations of royal jelly in tris extender on motility, viability, morphological abnormalities and membrane integrity of spermatozoa in Arabi rams. For this purpose, the semen of 10 Arabi rams were polled and then examined under 5 treatments including the tris diluent containing the concentrations of zero, 0.5, 1, 1.5 and 2% royal jelly in three times of zero, 24 and 48 hours in a completely randomized design. The results showed a significant difference in motility of spermatozoa among royal jelly treatments and control (P<0.05). At 0.5, 1 and 1.5% of royal jelly, sperm motility was significantly more than the control group (P<0.05). Sperm viability rate was not affected by treatments. The used concentrations of royal jelly have not significantly effect on the amount of morphological abnormalities of Arabic ram spermatozoa  in comparison with control group. Between different concentrations of royal jelly treatments and control group, there was not significant difference in the sperm plasma membrane integrity (P>0.05). Concentrations 1 and 1.5% of royal jelly improved the amount of sperm plasma membrane integrity when compared with 0.5 and 2% of royal jelly concentrations (P<0.05). In general, according to this study, it is concluded that the low levels of royal jelly (0.5, 1 and 1.5%) improved the sperm progressive motility during liquid storage in Arabi ram.