Histochemical and histomorphometrical studies of mucous secreting cells in different regions of epidermis in Euryglossa orientalis fish

Erfani Majd, N; Ertefaat, S; Savary, A; Salamat, N

Histochemical and histomorphometrical studies of mucous secreting cells in different regions of epidermis in Euryglossa orientalis fish

Document Type : Research Paper

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Abstract

This study was done on the skin of 5 of 15 normal and mature female soleid fish with average weight of 790.4±170.41gr in reproductive season (September) from khoor Musa area. The fish were cached with gill net from mouse creek. In order to conduct a microscopical study, 0.5 cm thick samples were taken from different parts of the skin, including dorsal, lateral (on lateral line), ventral, head and tail. The samples were fixed in Bouin’s solution and 5-6 µm thick sections were made using routine paraffin embedding method and were stained by H&E, Alcian blue (AB) pH 2.5 and PAS. The histochemical results showed that there are 2 types of mucous secreting cells (acidic and neutral) in epidermis of Soleid fish. The acidic mucous secreting cells were stained blue in AB staining and neutral mucous secreting cells were stained purple in PAS staining and they were recogonized from other cells easly. Histometrical results showed that the number and distribution of acidic mucous cells varied in different regions of skin, so that these cells were more in ventral region than dorsal and head (P<0.05) but there were no significant differences between the number of neutral cells in different regions of skin (P>0.05).

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