Iranian Veterinary Journal

Iranian Veterinary Journal

In vitro antibacterial effects of several plant essential oils on Vibrio parahaehaemolyticus, Vibrio harveyi and Vibrio damsela

Document Type : Research Paper

Authors
Afsane Narouie 1
javad mirdar 2
ahmad gharaie 3
narjes sanchooli
1 M.Sc in the field of fisheris,Department of Fisheries, Faculty of Natural Resources, University of Zabol,jahad square, zabol
2 zabol university- Faculty of Natural Resources, University of Zabol
3 zabol university
10.22055/ivj.2016.38463
Abstract
For the charges effect of chemical drugs and limitation in their use, disposition use of medicinal plants has increased. In this study essential oils of three medicinal plants Mentha spicata, Eugenia caryophyllata and cuminum cyminum were subjected to in vitro antibacterial assay against fish pathogenic Vibrio parahaehaemolyticus, Vibrio harveyi and Vibrio damsel. Disc diffusion method was used to test antibacterial activity. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by using standard procedure Broth- Microdilution. Among three plants tested, E.caryophyllata (Clove) was found to be the most effective. All the plants were effective against all the tasted organisms. The largest zone of inhibition (12 mm) was obtained with E.caryophyllata against V. damsel and Minimum Bactericidal Concentration (MBC) value of 4 mg/ml was obtained with M. spicata and E.caryophyllata against V. parahaehaemolyticus, and V.harveyi were found to be resistant with all the plant essential oils. On the basis of investigation, we can say, E. caryophyllata could be used as a source of new antibacterial agent for developing drugs to inhibit some fish pathogenic bacteria.
Keywords
Vibrio harveyi
Eugenia caryophyllata
antibacterial
Minimum inhibitory concentration
Broth micro-dilution
Subjects