Iranian Veterinary Journal

Iranian Veterinary Journal

Seroprevalence of Neospora caninum in dogs from Khorramabad and Kermanshah, west of Iran

Document Type : Research Paper

Authors
Mohammad Hossein Abbasi 1
Hamidreza Shokrani 2
Saman Salmani 3
Bahman Mosallanejad 4
1 DVM Graduate, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
2 Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
3 Assistant Professor, Department of Clinical Science, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
4 Professor, Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
10.22055/ivj.2026.532217.2813
Abstract
    Neosporosis has a global distribution and a great impact on the dairy cow industry. This study was aimed to determine seroprevalence and risk factors of Neospora caninum infection in owned and homeless dogs from Khorramabad and Kermanshah areas, west of Iran. A total number of 120 dogs (60 from Khorramabad and 60 from Kermanshah) were randomly selected. Blood samples were taken from cephalic vein and sera were analyzed for detection of anti-N. caninum antibodies using Neospora modified agglutination test (N-MAT). Of all, 18 (15%) of the examined dogs were infected with N. caninum. The seroprevalence of infection was higher in Kermanshah (16.66%) than in Khorramabad (13.33%). There was no significant difference in the seroprevalence among household dogs (27.27%), farm dogs (16.66%), and homeless dogs (12.65%). It is critical in these areas to implement the necessary measures to control the parasite and manage the population of dogs with N. caninum infection.
Keywords
Neospora caninum
Seroprevalence
dog

Subjects

Altaee, M. N., Sray, A. H., & Ashur, K. H. (2022). First serological investigation of Neospora caninum in stray dogs, Iraq. Biochemical and Cellular Archives, 22(1), 3007-3012.
Anvari, D., Saberi, R., Sharif, M., Sarvi, S., Hosseini, S. A., Moosazadeh, M., Hosseininejad, Z., Chegeni, T. N., & Daryani, A., (2020). Seroprevalence of Neospora caninum infection in dog population worldwide: a systematic review and meta-analysis. Acta Parasitologica, 65, 273–290.
 Ayati R., Rezvan H., Namavari M. M., Sadeghi-nasab A., Hamoon Navard S., & Dadmehr B. (2023). Association between presence of DNA and antibody in the serum during vertical transmission of Neospora caninum. Iranian Veterinary Journal, 19(1), 16–23.
Barker, A., Wigney, D., Child, G., & Šlapeta, J. (2021). Seroprevalence of Neospora caninum in dogs from greater Sydney, Australia unchanged from 1997 to 2019 and worldwide review of adult-onset of canine neosporosis. Current Research in Parasitology & Vector-Borne Diseases, 1, 100005.
Bjerkås, I., Mohn, S. F., & Presthus, J. (1984). Unidentified cyst-forming sporozoon causing encephalomyelitis and myositis in dogs. Zeitschrift für Parasitenkunde, 70(2), 271–274.
Dubey, J. P. (2003). Review of Neospora caninum and neosporosis in animals. Korean Journal of Parasitology, 41(1), 1–16.
Dubey, J. P., Alvarado-Esquivel, C., Liesenfeld, O., Herrera-Flores, R. G., Ramírez-Sánchez, B. E., González-Herrera, A., Martínez-García, S. A., Bandini, L. A., & Kwok, O. C. H. (2007). Neospora caninum and Toxoplasma gondii antibodies in dogs from Durango City, Mexico. Journal of Parasitology, 93(5), 1033–1035.
Gharekhani, J., & Yakhchali, M. (2019). Neospora caninum infection in dairy farms with history of abortion in West of Iran. Veterinary and Animal Science, 8, 100071.
Gharekhani, J., Yakhchali, M., & Berahmat, R. (2020). Neospora caninum infection in Iran (2004–2020): A review. Journal of Parasitic Diseases, 44(4), 671–686.
Haddadzadeh, H. R., Sadrebazzaz, A., Malmasi, A., Talei Ardakani, H., Khazraii Nia, P., & Sadreshirazi, N. (2007). Seroprevalence of Neospora caninum infection in dogs from rural and urban environments in Tehran, Iran. Parasitology Research, 101, 1563–1565.
Hariri, M., Arefkhah, N., Ghorbani, F., Namavari, M., Omidian, M., &  Sarkari, B. (2021). Molecular and serological evaluation of Neospora caninum infection in dogs from a rural setting in Fars province, southern Iran. Iranian Journal of Parasitology, 16(1), 146.
Hazrati Kalbi Beki, F., Azizi, H., Kojouri, G. A., Pirali, Y., & Hossininejad, M. (2023). Seroprevalence of Neosporosis in goats and farm dogs at the central Zagros region (Iran). Iranian Veterinary Journal, 19(3), 41–44.
Hosseininejad, M., Hosseini, F., Mahzounieh, M., Raisi Nafchi, A., & Mosharraf, M. (2010). Seroprevalence of Neospora caninum infection in dogs in Chaharmahal-va-Bakhtiari Province, Iran. Comparative Clinical Pathology, 19, 269–270.
Kennedy, A., White, J. D., & Child, G. (2024). Neosporosis in 21 adult dogs, 2010-2023. Journal of Veterinary Internal Medicine, 38(6), 3079–3086.
Khordadmehr, M., Hosseini, S., Mohsenifar, E., Namavari, M., & Khordadmehr, S. (2012). Seroprevalence of Neospora caninum in farm and household dogs determined by ELISA. Online Journal of Veterinary Research, 16(4), 172–81.
Morganti, G., Rigamonti, G., Brustenga, L., Calgaro, V., Angeli, G., Moretta, I., Diaferia, M., & Veronesi, F. (2024). Exploring similarities and differences between Toxoplasma gondii and Neospora caninum infections in dogs. Veterinary Research Communications, 48(6), 3563–3577.
Mosallanejad, B., Bahrami, S., Hamidinejat, H., & Ghanavati, S. (2018). A serological survey of Neospora caninum infection in urban and rural dogs in Ahvaz district, Southwest of Iran. Archives of Razi Institute, 73(3), 215–221.
Motamedi, A., Keihani, P., & Momtaz, H. (2020). Molecular detection of Neospora caninum in infected dogs of Isfahan, Iran. Journal of Zoonotic Diseases, 4(1), 35–42.
Nematollahi, A., & Foroozanfar, N. (2023). A review of the occurance of bovine neosporosis in Iran. Veterinary Clinical Pathology, 17(65).
Nematollahi, A., Shahbazi, P., & Fakheri, A. (2022). Study on Prevalence Rate of Neospora caninum in Dogs Around Tabriz Through Fecal and Molecular Methods. Journal of Veterinary Research, 76(4), 381–388.
Reichel, M. P., Wahl, L. C., & Ellis, J. T. (2020). Research into Neospora caninum—what have we learnt in the last thirty years?. Pathogens, 9(6), 505.
Romand, S., Thulliez, P., & Dubey, J. P. (1997). Direct agglutination test for serologic diagnosis of Neospora caninum infection. Parasitology Research, 84, 50–53.
Salama, D. B., Fereig, R. M., Abdelbaky, H. H., Shahat, M. S., Arafa, W. M., Aboelhadid, S. M., Mohamed, A. E., Metwally, S., Abas, O., Suo, X., & Gupta, N. (2022). Toxoplasma gondii and Neospora caninum antibodies in dogs and cats from Egypt and risk factor analysis. Pathogens, 11(12), 1464.
Smith, B. J. (1999). Canine anatomy. 1st ed. Philadelphia: Lippincott Williams & Wilkins
Turlewicz-Podbielska, H., Ruszkowski, J. J., Wojciechowski, J., & Pomorska-Mól, M. (2024). Seroprevalence of Neospora caninum in pet cats, dogs and rabbits from urban areas of Poland. BMC Veterinary Research, 20(1), 37.