Iranian Veterinary Journal

Iranian Veterinary Journal

Evaluation of specific immunogenicity of Aeromonas hydrophila biofilm oral vaccine in common carp

Document Type : Research Paper

Authors
Amir Aramoon 1
Mojtaba Alishahi 2
Masoudreza Seifi Abad Shapoori 3
Masoud Ghorbanpoor 4
1 PhD Graduate in Aquatic Animal Health and Diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4 Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran, and Department Pathbiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
10.22055/ivj.2023.415944.2636
Abstract
    One of the methods of protection and slow release of vaccine antigens is the use of biodegradable natural polymers such as chitin in the bacterial biofilm substrate. The bacterium is complex and protected by the polymer substrate in the conditions of the digestive system, and finally, healthier vaccine antigens with better immunogenicity are provided to the intestine mucosal immune system of the fish. In this method, biofilm vaccine of Aeromonas hydrophila bacteria was done by culturing the bacteria in TSB medium (0.225% W/V) along with chitin (0.3% W/V) for 4 days in a shaker incubator. Afterward bacteria were washed with sterile PBS and were inactivated by heat. A number of common carp of 32 ± 11 g were divided into 5 equal treatments and each treatment was repeated three times as follows: the first to fourth treatment with biofilm vaccine, bacterin, media chitin and chitin + TSB respectively, by oral method during 10 days were immunized; the fifth treatment without vaccine administration was considered as the control group. Sampling of fish serum was done on days 0, 20, 40 and 60. Antibacterial antibody level in the samples was evaluated by ELISA test and by monoclonal antibody. The results showed that the antibody level of Aeromonas hydrophila in oral biofilm treatment on day 20 was significant compared to the other treatments, but it was not significant on days of 40 and 60.
Keywords
Oral vaccine
Biofilm
Aeromonas hydrophila
Common carp

Subjects

Abhiman, M.F.Sc, (2014). Effect of Aeromonas hydrophila biofilm oral vaccine on gut immunity of carps. PhD Thesis Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar. P 86.
Ahangarzadeh, Gurbanpour Najafabadi, Massoud, Peighan, Sharif Rouhani, & Soltani. (2015). The role of Aeromonas hydrophila in bacterial septicemia of farmed carp fish in Khuzestan province. Iranian Veterinary Journal, 11(3), 5-16. (In Persian)
Aramoon, A., Alishahi, M., Seifi Abad Shapouri, M. R., & Ghorbanpour, M. (2018). Immunogenicity evaluation of Aeromonas hydrophila biofilm vaccine on the fingerling Common carp (Cyprinus carpio). Journal of Animal Environment, 10(3), 207-212.‏ (In Persian)
Azad, I.S  Shankar K.M,  Mohan C.V And,  Kalita, B. (2000). Uptake and processing of biofilm and free-cell vaccines of Aeromonas hydrophila in Indian major carps and common carp following oral vaccination antigen localization by a monoclonal antibody. Disease of Aquatic Organisms. 43: 103–108.
Azad, I.S  Shankar K.M,  Mohan C.V And,  Kalita, B. (1999). Biofilm vaccine of Aeromonas hydrophila– standardization of dose and duration for oral vaccination of carps. Fish and shellfish immunology, 519-528.
Boyd A, Chakrabarty AM. (1995). Pseudomonas aeruginosa biofilms: role of the alginate exopolysaccharide. J Indust Microbiol, 15:162-168.
Dhar AK, Manna SK, Thomas Allnutt FC. (2014). Viral vaccines for farmed finfish. Virusdisease, 25(1):1–17.
Dong, L., Wichers, H. J., & Govers, C. (2019). Beneficial health effects of chitin and chitosan. Chitin and chitosan: Properties and applications, 145-167.‏
Gopalakannan, A. and Arul, V. (2006). Immunomodulatory effects of dietary intake of chitin, chitosan and levamisole on the immune system of Cyprinus carpio and control of Aeromonas hydrophila infection in ponds. Aquaculture, 255,179–187.
Hall-Stoodley L, Costerton JW, Stoodley P. (2004). Bacterial biofilms: from the natural environment to infectious diseases. Nat Rev Microbiol, 2:95-108.
Kaur, B., Kumar, B. N., Tyagi, A., Holeyappa, S. A., & Singh, N. K. (2021). Identification of novel vaccine candidates in the whole-cell Aeromonas hydrophila biofilm vaccine through reverse vaccinology approach. Fish & Shellfish Immunology, 114, 132-141.‏
Kuzmina, V.V., Golovanova, I.L., Izvekova, G.I. (1996). Influence of temperature and season on some characteristics of intestinal mucosa carbohydrases in six freshwater fishes. Comp Biochem Physiol , 113: 255-60.
Landry, R.M., An, D., Hupp, J.T., et al. (2006). Mucin–Pseudomonas aeruginosa interactions promote biofilm formation and antibiotic resistance. Mol Microbiol; 59:142–151.
 Lee, H.J., Seo, J., Kim, M. S., Lee, Ch. (2017). Inactivation of biofilms on RO membranes by copper ion in combination with norspermidine. Desalination, 424 , 95–101.
Lindsay, D., von Holy, A. (2006). Bacterial biofilms within the clinical setting: what healthcare professionals should know. Journal of Hospital Infection, 64, 313-325.
Manjakasy JM, Farr I, Hansen MJ, Tibbetts IR. (2011). Enzymatic digestion in stomach less fishes: how a simple gut accommodates both herbivory and carnivory. J Comp Physiol B;181:603e13.
Nayak DK, Asha A, Shankar KM, Mohan CV. (2004). Evaluation of biofilm of Aeromonas hydrophila for oral vaccination of Clarias batrachusda carnivore model. Fish & Shellfish Immunology. 16, 613-619.
Plant, K. P., & LaPatra, S. E. (2011). Advances in fish vaccine delivery. Developmental & Comparative Immunology, 35(12), 1256-1262.‏
Siriyappagouder, P., Shankar, K. M., Kumar, B. N., Patil, R., & Byadgi, O. V. (2014). Evaluation of biofilm of Aeromonas hydrophila for oral vaccination of Channa striatus. Fish & Shellfish Immunology, 41(2), 581-585.
‏ Sharma, K.S.R., Shankar, K.M., Sathyanarayana, M.L., Patil, R.R., Swamy, N. and Rao, S. (2010a). Development of biofilm of Vibrio alginolyticus for oral immunostimulation of shrimp. Aquacul. Intl. , 19:421–430.
Sharma, K.S.R., Shankar, K.M., Sathyanarayana, M.L., Sahoo, A.K, Swamy, N. and Rao, S. (2010b). Evaluation of immune response and resistance to diseases in tiger shrimp, Penaeus monodon fed with biofilm of Vibrio alginolyticus. Fish Shellfish Immunol, 29:724–732.
Swaminathan, T.R.; Rathore, G.; Abidi, R. and Kapoor, D. (2004). Detection of Aeromonas hydrophila by polymerase chain reaction. Indian Journal of Fisheris, 51(2): 251-254.
Tavakoli, H., Akhlaghi, M. (2015). Evaluation of changes in lysozyme, immunoglobulins, cells and blood hematocrit cultivated rainbow trout after experimental infection with pathogenic Aeromonas hydrophila. Biological Journal of Microorganism; 4(13): 93-104.
Tokmechi A., Shamci H., Meshkini S., Delshad R., Ghasemimaghanjoghi A. Dietary administration of vitamin C and Lactobacillus rhamnosus in combination enhanced the growth and innate immune response of the rainbow trout, Oncorhynchus mykiss. Journal of Iranian Fisheries 2012; 21(2): 13-22.
Vijavabaskar, P., Somasundaram, S. (2008). Isolation of bacteriocin producing lactic acid bacteria from fish gut and probiotic against common fresh water fish pathogen. Aeromonas hydrophila. Biotechnology; 7(1): 124-128.
Vinay TN, Rajreddy P, Suresh Babu PP, Rajesh R and Shankar KM. (2013). Evaluation of the Efficiency of Aeromonas hydrophila Biofilm Vaccine in Labeo rohita Employing Monoclonal Antibody based ELISA. Open Access Scientific Reports. 1-4.
Zambonino Infante, J.L., Cahu, C.L. (2001). Ontogeny of the gastrointestinal tract of marine fish larvae. Comp Biochem Physiol; 130: 477-87.