Iranian Veterinary Journal

Iranian Veterinary Journal

Positive effects of acellular aortic scaffold on antioxidant activities and cellular homeostasis: an experimental study on cardiomyocytes of infarcted mice

Document Type : Research Paper

Authors
mahara hosseinabadi 1
Zohreh Abdolmaleki 2
Seyedeh Parastoo Yasini 3
1 DVM Graduated, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran
2 Assistant Professor, Department of Pharmacology, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran
3 Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran
10.22055/ivj.2024.401904.2639
Abstract
    Myocardial infarction (MI) has increased in the recent years due to aging as well as lifestyle changes in the population. In the last decades, regenerative medicine has been considered to provide modern and efficient methods for MI patients' therapies. The current study was aimed at investigating the effect of acellular aortic scaffold (AAS) on MI cardiomyocytes to identify the therapeutic potential of this natural biomaterial for MI patients. The study was conducted in five main steps: Preparing MI animal model, preparing MI cells, culture of animal MI cell on AAS, assessment of cell viability (MTT assay) as well as antioxidant and catalytic activities (ROS, TAC, SOD, GPX, and CAT assays) and molecular study on apoptotic and anti-apoptotic factors (qRT-PCR). AAS positively affected the viability rate of MI cells and the GPX and SOD levels were significantly increased in MI cells due to the culture on AAS. The RT-PCR quantification showed a decrease in the levels of of Cox8 and Caspase3 expression genes levels in MI cells, cultured on AAS, while an increase in miR-24 expression level was observed in MI cells as a result of culture on AAS. We concluded that acellular aortic scaffold can positively control the catalytic and antioxidant activities, cellular hemostasis, and cell viability of cardiomyocytes after myocardial infarction, demonstrating the potential of such natural biomaterials for cardiac tissue reconstruction. However, to achieve favorable results and ideal therapeutic applications, further studies are required.
Keywords
Myocardial infarction
Regenerative medicine
Acellular aortic scaffold
Antioxidant Activity

Subjects

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