Protein pattern of Yersinia ruckeri isolates from farmed rainbow trout (Onchorhyncus mykiss)

Bahadori, Nastaran; Soltani, Mehdi; Farahmand, Mahnaz; Mohammadian, Samira; Soltani, Elahe

doi:10.22055/ivj.2016.14696

Protein pattern of Yersinia ruckeri isolates from farmed rainbow trout (Onchorhyncus mykiss)

Document Type : Research Paper

Authors

nastaran bahadori ¹

Mehdi Soltani ²

mahnaz farahmand

samira mohammadian

Elahe Soltani ³

¹ student

² Professor, Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran,, Tehran, Iran

³ BSc Student of Microbiology, Facullty of Biology, University of Tehran, Tehran, Iran

10.22055/ivj.2016.14696

Abstract

Yersiniosis is one of the most important fish bacterial diseases cause by of Yersinia ruckeri. Isolation, characterization and of protein profile of Yersinia ruckeri were studied in some affected rainbow trout farms in Iran. The kidney and spleen samples from 16 fish farms in Charmahal-va-Baakhteyiari, Mazandaran and Qazvin provinces were cultured on blood agar at 22⁰C for 48 hours and the grown bacteria were characterized by phonotypical and molecular studies. Biochemical and molecular works resulted in identification of six isolates of Yersinia ruckeri. The whole proteins of the six recovered isolates of Yersinia ruckeri subjected to sodium dodecyl sulphat - poly acrylamide gel electrophoresis (SDS-PAGE) showed that the protein profiles of 5 isolates were highly similar to each other and only one isolates showed 5% difference with other isolates. Therefore, these results show that some farmed trout in Iran are affected by Yersinia ruckeri isolates of high homology. Also, such results could be useful for protection aims such as immunization.

Keywords

Yersinia ruckeri

Yersiniosis

rainbow trout and SDS-PAGE

Subjects