Document Type : Research Paper
Authors
1
Associate Professor, Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2
MSc Graduated of Animal Physiology, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3
Professor, Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4
PhD Student of Animal Physiology, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
5
Assistant Professor, Department of Animal Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Abstract
The aim of this study was to determine the effects of different pre-freezing equilibration times (2, 4 and 6 h) on quality parameters if post-thawed buck semen using soybean lecithin supplemented extender. In this study, semen samples were collected from four Mahabadi bucks. After collection, semen samples were primarily evaluated and pooled together. Afterward, pooled semen samples were divided into three equal parts and diluted in semen extender containing soybean lecithin. Then, after spending different equilibration times diluted semen samples were frozen. After thawing, sperm motility characteristics, plasma membrane integrity and functionality, abnormality and lipid peroxidation were evaluated. Base on the obtained results showed that six h-equilibration time resulted in higher progressive motility (25.6 ± 1.39 %) compared to other groups (P<0.05). the other sperm quality parameters did not alter by different equilibration times. In conclusion, spending six h-equilibration time improved only sperm progressive motility. Also, doing other professional evaluations and fertility are recommended.
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