Determination of genetic diversity and susceptibility of Trichophyton mentagrophytes isolates against antifungal effects of ethanolic extract of Allium jesdianum

Document Type : Research Paper

Authors

1 MSc Graduated of Mycology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Assistant Professor, Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Phd Graduated of Mycology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

4 Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

5 Professor, Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

Dermatophytes are a group of fungi with keratinophilic properties. They are able to invade epidermis, especially stratum corneum, by production of proteolytic enzymes such as keratinase. Trichophyton mentagrophytes (T. mentagrophytes) is a common cosmopolitan dermatophyte species. This species has different varieties based on genetic characteristics. In this study genetic diversity of 20 clinical isolates of T. mentagrophytes and their susceptibility to ethanolic extract of Allium jesdianum (A. jesdianum) were evaluated. Genetic diversity of the isolates was assessed by Random Amplified Polymorphic DNA (RAPD) method using 4 primers including OPN16, A08, OPD18 and R28. Then, susceptibility of T. mentagrophytes isolates to A. jesdianum extract was determined by standard microdilution broth method. The MIC values of the isolates were between 6.1 and 49.3 mg/ml (mean MIC: 20.3 mg/ml), whereas the MFC values of the isolates were between 24.6 and 49.3 mg/ml (mean MFC: 40.6 mg/ml). Twenty eight propagated DNA fragments were achieved in RAPD-PCR reaction. The most propagated DNA fragments (9 bands) were related to OPD18 primer. The R28 primer showed the least propagated DNA fragments (5 bands). Drawn dendrogram identified 3 main groups among 20 T. mentagrophytes isolates according to the all primers in 65% genetic distance. Most of T. mentagrophytes isolates (75%) were placed in group 2 and 100% genetical similarity was only seen between 2 isolates. It was concluded that all T. mentagrophytes genotypes were susceptible to A. jesdianum. The correlation between antifungal susceptibility and T. mentagrophytes genotype may be of potential therapeutic significance and larger studies are needed to prove this finding.

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References

Aala, F.; Yusuf, U.K.; Khodavandi, A. and Jamal, F. (2010). In vitro antifungal activity of allicin alone and in combination with two medications against six dermatophytic fungi. African Journal of Microbiology Research, 4 (5): 380-385.
Amiri, H. (2007). Chemical composition and antibacterial activity of the essential oil of Allium jesdianum Boiss. & Buhse from Iran. Journal of Medicinal Plants, 1 (3): 39-44.
Amiri, H. (2007). Identification of chemical composition and antibacterial activity of the essential oil and different extracts of Allium jesdianum. Journal of Medicinal Plants, 6: 39-44.
Emilie, E. and Marion, R. (2007). Phylogenetic analysis of Trichophyton mentagrophytes human and animal isolates based on MnSOD and ITS sequence comparison. Microbiology, 153 (10): 3466-3477.
John, H.R.; Mahmood, A.G. (2008). Clinical and Laboratory Standards Institute (CLSI). Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; Approved standard, 2nd ed, CLSI document M38-A2, Clinical and Laboratory Standards Institute, Wayne, PA.
Hasanein, P. and Gomar, A. (2014).  Effects of Zingiber officinale Rosc. hydroethanloic extract on antinociception induced by hyoscine in male rats. Journal of Medicinal plants, 2 (50): 172-179.
Kanbe, T. (2008). Molecular approaches in the diagnosis of dermatophytosis. Mycopathologia, 166 (5-6): 307-317.
Kauffman, C.A.; Pappas, P.G.; Sobel, J.D. and Dismukes, W.D. (2011). Essentials of Clinical Mycology. 2nd ed., London, UK: Springer Science Business Press, Pp: 207-209.
Khosravi, A.R. (2008). Fungal infections and immune responses. 1st ed. Tehran, Iran: Tehran University Press, Pp: 34-38.
Khosravi, A.R. (2009). Medical Mycology, A practical approach. 2nd ed. Tehran, Iran: Jahad-e- Daneshgahi of Tehran Press, P: 56-59.
Khosravi, A.R.; Shokri, H.; Rostami, A.; Tamai, I.A.; Erfanmanesh, A. and Memarian, I. (2012). Severe dermatophytosis due to Trichophyton mentagrophytes var. interdigitale in flocks of green iguanas (Iguana iguana). Journl of Small Animal Practice, 53: 286-291.
Kim, J.A.; Takahashi, Y.; Tanaka, R.; Fukushima, K.; Nishimura, K. and Miyaji, M. (2001). Identification and subtyping of Trichophyton mentagrophytes by random amplified polymorphic DNA. Mycoses, 44 (5):157-165.
Martinez-Rossi, N.M.; Peres, N.T.A. and Rossi, A. (2008). Antifungal resistance mechanisms in dermatophytes. Mycopathologia, 166 (5-6): 369-383.
Mercy, K.A.; Ijeoma, I. and Emmanuel, K.J. (2014). Anti-dermatophytic activity of garlic (Allium sativum) extracts on some dermatophytic fungi. International Letters of Natural Sciences, 19: 34-40.
Mirhendi, H.; Nooripour Sisakhet, S.; Shidfar, M.R.; Zaini, F.; Jalalizand, N. and Tavakoli, F. (2009). Identification and differentiation of Trichophyton Mentagrophytes and T. rubrum by polymerase chain reaction and enzymatic digestion. Ofogh Danesh, 13: 39-48.
Mirzahoseini, H.; Omidinia, E.; Shams-Ghahfarokhi, M.; Sadeghi, G. and Razzaghi-Abyaneh, M. (2009). Application of PCR-RFLP to rapid identification of the main pathogenic dermatophytes from clinical specimens. Iranian Journal of Public Health, 38 (1): 18-24.
Mochizuki, T.; Sugie, N. and Uehara, M. (1997). Random amplification of polymorphic DNA is useful for the differentiation of several onthropophlic dermatophytes. Mycoses, 40 (11-12): 405-409.
Spesso, M.F.; Nuncira, C.T.; Burstein, V.L.; Masih, D.T.; Dib, M.D. and Chiapell, L.S. (2013). Microsatellite-primed PCR and random primer amplification polymorphic DNA for the identification and epidemiology of dermatophytes. European Journal of Clinical Microbiologyand Infectious Diseases, 32 (8): 1009-1015.
Sharifzadeh, A. and Shokri, H. (2016). Antifungal activity of essential oils from Iranian plants against fluconazole-resistant and fluconazole-susceptible Candida albicans. Avicenna Journal of Phytomedicine, 6 (2): 215-222.
Yang, X.; Sugitac, T.; Takashimad, M.; Hirumae, M.; Li, R. and Sudoa, H. (2009). Differentiation of Trichophyton rubrum clinical isolates from Japanese and Chinese patients by randomly amplified polymorphic DNA and DNA sequence analysis of the non-transcribed spacer region of the rRNA gene. Journal of Dermatological Science, 54 (1): 38-42.
Yazdanparast, S.A.; Rahimi, H. and Farnoodian, M. (2009). Molecular strain typing of Trichophyton mentagrophytes isolated from patients with dermatophytosis by random amplified polymorphic DNA. Journal of Iran University Medical Sciences, 16 (66): 36-42.

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