concentrations of Royal Jelly supplementation in tris diluent on semen quality parameters of Arabi ram

Raeesi Dehkohneh, Soraya; Mamouei, Morteza; Tabatabaei, Saleh; Fayazi, Jamal; Naghibi Rokni, Hessam

doi:10.22055/ivj.2017.30765.1564

concentrations of Royal Jelly supplementation in tris diluent on semen quality parameters of Arabi ram

Document Type : Research Paper

Authors

¹ Graduate student of Animal Physiology

² Associate Professors, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran

³ Assistant Professors Department of Animal Sciences, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran

⁴ MSc of Animal Physiology, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran

10.22055/ivj.2017.30765.1564

Abstract

This experiment was conducted to investigate the effects of different concentrations of royal jelly in tris extender on motility, viability, morphological abnormalities and membrane integrity of spermatozoa in Arabi rams. For this purpose, the semen of 10 Arabi rams were polled and then examined under 5 treatments including the tris diluent containing the concentrations of zero, 0.5, 1, 1.5 and 2% royal jelly in three times of zero, 24 and 48 hours in a completely randomized design. The results showed a significant difference in motility of spermatozoa among royal jelly treatments and control (P<0.05). At 0.5, 1 and 1.5% of royal jelly, sperm motility was significantly more than the control group (P<0.05). Sperm viability rate was not affected by treatments. The used concentrations of royal jelly have not significantly effect on the amount of morphological abnormalities of Arabic ram spermatozoa in comparison with control group. Between different concentrations of royal jelly treatments and control group, there was not significant difference in the sperm plasma membrane integrity (P>0.05). Concentrations 1 and 1.5% of royal jelly improved the amount of sperm plasma membrane integrity when compared with 0.5 and 2% of royal jelly concentrations (P<0.05). In general, according to this study, it is concluded that the low levels of royal jelly (0.5, 1 and 1.5%) improved the sperm progressive motility during liquid storage in Arabi ram.

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