Document Type : Research Paper
Authors
1
PhD Student of Aquatic Health, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2
Associated Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3
Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4
Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
5
Graduated student of Aquatic Health, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract
It seems that the natural immune stimulants such as herbal extracts containing carotenoids, especially algae, is the most practical method to prevent fish disease.
Immune stimulants by enhancing the innate immune system can prevent the occurrence of infectious diseases. Dietary with carotenoids, has improved the immune system in many laboratory animals. In order to investigate the effect of alcoholic extract of Sorgassum angustifolium, 120 pieces of fish were randomly divided into four groups (each aquarium containing 30 piece fish, 6.5 ±0.65gr) and for 60 days for doing the experiment were maintenanced and fed. The fourth treatment was the control group that only received commercial food (Biomar) was coated with olive oil. First, second and third groups were fed with of 5, 10 and 15 g/kg ethanol extract S. angustifolium for two months. After 60 days, the fish were anesthetized and blood samples were taken for hematological and immunological studies. Among the different treatments, the highest rate of PCV, Hb and MCHC was observed in treatment that fed 5g/ kg algae (p<0.05).
The number of white blood cells in all treatments were fed with algae was more than the control group, but this difference was not significant (p>0.05). NBT activity in the serum of treatment fed with 15g/kg S. angustifolium algae was more than the other treatments and the control group (p<0.05). Complement activity in groups that fed with 10 and 15g/ kg alcoholic extract of algae in day 60, was significantly higher than the control group (p<0.05). Serum bactericidal power in treatments that fed with alcoholic extract of algae, compared to other treatments and control group showed no significant difference (p>0.05). According to the results of the present study, oral administration of ethanol extract of S. angustifolium stimulate some nonspecific immune factors in Macro fish.
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