The goal of this study was to compare the effect of different forms of L. casei as a probiotic bacterium on the growth and immunity of rainbow trout. For this purpose, four hundred and eighty rainbow trout (50±6.5 g initial weight) were prepared and acclimatized to the laboratory conditions for 10 days and then the fish randomly divided into four groups. One group selected as control and fed with a commercial diet without any supplementation. Other groups received commercial supplemented diet with live, lyophilized and heat killed forms of L. casei at concentration of 5×108 CFU/gr, respectively. Duration of the study was 45 days and sampling for biometry and humeral immunity (serum lysozyme activity, alternative complement activity and total antibody) were conducted at the day of 0, 15, 30 and 45. Results showed that the amount of final weight, weight gain percentage and specific growth rate statistically (P<0.05) were higher at the fish fed with live (fresh culture and lyophilized) and heat killed form of L. casei than the control. Also, the amounts of these parameters were higher at the fish fed lyophilized form of bacteria than other treatments. Immune assay showed serum lysozyme activity, alternative complement pathway and the level of serum total antibody increased at days 15 and 30 after receiving the different forms of L. case. The elevation of these parameters were significant (P<0.05) in fish fed with the lyophilized form of bacteria than the control. Also, results showed that the activity of lysozyme, alternative complement pathway and serum total antibody decreased in fish fed different forms of bacteria on day 45 (15 days after feeding withdrawal). Based on obtained results, it is concluded that addition of lyophilized form of L. casei into the rainbow trout diet could improve growth parameters and enhanced immunity in this fish.
Oshnukhah, M., Tukmechi, A., Farokhi, F., & Manaffar, R. (2014). Comparative effect of different forms of Lactobacillus casei on growth and immunity in rainbow trout (Oncorhynchus mykiss). Iranian Veterinary Journal, 9(4), 25-35.
MLA
m Oshnukhah; A Tukmechi; f Farokhi; R Manaffar. "Comparative effect of different forms of Lactobacillus casei on growth and immunity in rainbow trout (Oncorhynchus mykiss)". Iranian Veterinary Journal, 9, 4, 2014, 25-35.
HARVARD
Oshnukhah, M., Tukmechi, A., Farokhi, F., Manaffar, R. (2014). 'Comparative effect of different forms of Lactobacillus casei on growth and immunity in rainbow trout (Oncorhynchus mykiss)', Iranian Veterinary Journal, 9(4), pp. 25-35.
VANCOUVER
Oshnukhah, M., Tukmechi, A., Farokhi, F., Manaffar, R. Comparative effect of different forms of Lactobacillus casei on growth and immunity in rainbow trout (Oncorhynchus mykiss). Iranian Veterinary Journal, 2014; 9(4): 25-35.
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