Study of the effect of the essential oil of anise (Pimpinella anisum) on Streptococcus iniae and Lactococcus garvieae isolates identified by PCR

Document Type : Research Paper

Authors

1 PhD Graduated from Aquatic Animal Health, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran

2 Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran

3 Professor, Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

10.22055/ivj.2022.341161.2464

Abstract

    The essential oils are a combination of volatile oils that are made into plants as a secondary metabolite. Anise essential oil is known for its antimicrobial activity to several bacteria pathogenic. Bacterial pathogens are one of the important factors in the aquaculture industry. The aim of this study was to identify the bacterial pathogens in Rainbow trout (Oncorhynchus mykiss) fish and also to investigate antibacterial activity of anise essential oil on these bacteria. In this study, two pathogenic bacteria in fish including Streptococcus iniae and Lactococcus garviae were isolated from head kidney then purified from fish and identified by PCR; then, the antibacterial activity of anise essential oil against these pathogens was evaluated by macrodilution broth method. According to the findings PCR results indicate high prevalence of Streptococcus iniae and Lactococcus garviae in rainbow trout culturing ponds. The effect of essential oil anise on bacteria showed a minimum growth inhibitory concentration for Streptococcus iniae about 0.19 µl/ml and this amount was 0.312 µl/ml for Lactococcus garviae. The diameter of the growth inhibition zone under the influence of essential oil of anise on these gram positive bacteria was compared with the diameter of the growth inhibition zone under the influence of common antibiotics the results showed a significant difference in anise essential oil compared with antibiotics. The result also showed significant potential of anise to inhibiting growth of these bacteria. so, identification by molecular methods can be an effective role in the success of aquaculture.

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References

Abdel-Reheem, M. A., & Oraby, M. M. (2015). Anti-microbial, cytotoxicity, and necrotic ripostes of Pimpinella anisum essential oil. Annals of Agricultural Sciences, 60(2), 335-340.
Austin B., Austin D.A. 2016. Bacterial fish pathogens, disease of farmed and wild fish (6th Ed.). Switzerland: Springer. Bahadori N., Soltani M., Farahmand M., Mohammadan S., Soltani E. 2016. Protein pattern of Yersinia ruckeri isolates in some farmed rainbow trout (onchorhynchus mykiss). Iranian Journal of Veterinary Research 12 (1), 5-12
Bakkali, F. Averbeck, S.Averbeck D, Idaomar M. Biological effects of essential oils: a review. Food Chem Toxicol 2008;46(2):446-75.
Bhandari, A., Bhat, R. A. H., Tandel, R. S., Dash, P., Shah, T. K., Ganie, P. A., & Sarma, D. (2019). Investigation of acute toxicity and behavioural changes on Oncorhynchus mykiss, rainbow trout fry in response to ethanolic extract of Myrica esculenta. Phar. Inno. J, 8, 807-810.
Buhner, SH. Herbal antibiotics: natural alternatives for treating drug-resistant bacteria. 2nd ed. North Adams: Storey Publishing; 2012.
Buller, N. (2014). Bacteria and fungi from fish and other aquatic animals: a practical identification manual, Department of Agriculture and Food Western Australia. (2nd edi.). Includes bibliographical references and index. ISBN 978-1-84593-805-5
Carson, J. Wilson, T. (2009). Yersiniosis in fish. Aust New Zeal Stand Diagnostic Procedure 1-19.
Chahardoli, A. Foroughi,A. Nooriyan-soroor, M. E. (2020). in vitro Effects of essential oil and alcoholic extract of Anise (Pimipinella anisum) on Escherichia coli and Staphylococcus aureus. In Persia
Elshafie, S. S., Elshafie, H. S., El Bayomi, R. M., Camele, I., & Morshdy, A. E. (2022). Evaluation of the antimicrobial activity of four plant essential oils against some food and phytopathogens isolated from processed meat products in Egypt. Foods, 11(8), 1159.
Fathi-Achachlouei, B. Babolanimogadam, N. Zahedi, Y. (2020). Identification of Anise (Pimpinella anisum L.) Essential Oil Compounds and Investigation of its Effect on Some Foodborne Pathogens: Bacillus cereus, Staphylococcus aureus, Escherichia coli O157: H7and Salmonella typhimurium
Hassani, F. Payghan, R. Alishahi,M. Ghorpanpour,M. Ahangarzadeh, M. (2020). Molecular and biochemical investigation of the role of streptococcus iniae in mortality of Lates calcarifer cages culturing in the Persian Gulf. In Persia
Kalemba, D. A. A. K., & Kunicka, A. (2003). Antibacterial and antifungal properties of essential oils. Current medicinal chemistry, 10(10), 813-829
Mata, A. I, Gibello. A, Casamayor. A, Blanco. M. M. Domínguez, L. & Fernández-Garayzábal, J. F. (2004). Multiplex PCR assay for detection of bacterial pathogens associated with warm-water streptococcosis in fish. Appl. Environ. Microbiol., 70(5), 3183-3187.
Sharirfpour, I. Soltani, M. & Mazandarani, M. (2020). Histopathological features of infection by Streptococcus iniae in Persian sturgeon, Acipenser persicus. Iranian Journal of Aquatic Animal Health, 6(2), 39-48.
Soltani, M. & Tarahomi, M. (2008). Study of streptococcosis/lactococcosis in some farmed rainbow trout in Fars province, Iran. The first International Congress on Aquatic Animal Health Management and Diseases, Tehran, Iran. p.124.
Soltani, M. Ghodratnama, M. Taheri Mirghaed, A. Zargar, A. Rooholahi, Sh. (2013). The effect of Zataria multiflora Boiss and Rosmarinus officinalis essential oil on Streptococcus iniae isolated from Rainbow trout farms. Journal of Veterinary Microbiology. 9(1): 1-11. (in Persian)
Soltani, M., PIRALI, K. E., TAHERI, M. A., Zargar, A., Mohamadian, S., Roohollahi, S., & Zakian, M. (2015). Study on Streptococcosis and Lactococcosis outbreaks in rainbow trout farms in Fars and Lorestan Provinces.
Sun, Y., Hu, Y. H., Liu, C. S., & Sun, L. (2012). Construction and comparative study of monovalent and multivalent DNA vaccines against Streptococcus iniae. Fish & shellfish immunology, 33(6), 1303-1310.
Taheri Mirghaed, A.  Soltani,M.  Mahmoodi, Z.  Hosseini Shekarabi, P. (2016). Study of cultured rainbow trout contamination with Streptococcus iniae and Lactococcus garvieae in some fish markets of Tehran and Karaj (orginal reserch article). In Persia
Ullah, H., & Honermeier, B. (2013). Fruit yield, essential oil concentration and composition of three anise cultivars (Pimpinella anisum L.) in relation to sowing date, sowing rate and locations. Industrial Crops and Products, 42, 489-499.
Vazirzadeh, A., Jalali, S., & Farhadi, A. (2019). Antibacterial activity of Oliveria decumbens against Streptococcus iniae in Nile tilapia (Oreochromis niloticus) and its effects on serum and mucosal immunity and antioxidant status. Fish & Shellfish Immunology, 94, 407-416.
Vendrell, D., Balcázar, J. L., Ruiz-Zarzuela, I., De Blas, I., Gironés, O., & Múzquiz, J. L. (2006). Lactococcus garvieae in fish: a review. Comparative immunology, microbiology and infectious diseases, 29(4), 177-198.
Wang, Q. Zhang, C. Xu, L. Chen, J. Wang, X. (2020). Characterization of Streptococcus iniae ghost vaccine and its immunization in Nile tilapia (Oreochromis niloticus). Aquaculture Research. 2020;00:1–10. https://doi. org/10.1111/are.14990
Zheng, Y. Wu, W. Hu, G. Qiu, L. Meng, S. Song, C. Fan, L. Zhao, Z. Bing, X. & Chen, J. (2018). Gut microbiota analysis of juvenile genetically improved farmed tilapia (Oreochromis niloticus) by dietary supplementation of different resveratrol concentrations. Fish & Shellfish Immunology, 77, 200-207. https://doi.org/10.1016/j.fsi.2018.03.040
Zlotkin, A. Eldar, A. Ghittino, C. & Bercovier, H. (1998). Identification of Lactococcus garvieae by PCR. Journal of clinical microbiology, 36(4), 983-985.

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