Evaluation of an indirect hemagglutination method (IHA) with larval antigens for the serodiagnosis of Oestrus ovis infestations in goats

Document Type : Research Paper

Authors

1 Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Msc. Graduated, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

10.22055/ivj.2023.378594.2539

Abstract

    Oestrus ovis (sheep nasal bot-fly) is one of the important zoonotic parasites of small ruminants. Human ophtalmo- nasopharyngeal myiasis caused by O. ovis larvae have been reported in Iran and other countries. In this study, excretory-secretory (ES) and somatic (S) antigens of O. ovis larvae were evaluated for serodiagnosis of the infestation in goats by indirect haemagglutination (IHA). After tagging and blood sampling of goats in the slaughterhouse, the third and second larval stages of O. ovis (L2 and L3) were collected from their cut horns. For the preparation of somatic antigens (SL2 and SL3), the larvae were homogenized separately in test tube with a homogenizer, then centrifuged and the supernatant was collected. For the preparation of ES-antigens (ESL2/ESL3), the larvae were separately incubated in RPMI media with antibiotics for 48 hours. With 30 positive sera (from infested goats), 30 negative sera (from kids) and the antigens, the IHA method was evaluated for diagnosis of O. ovis infestation in goats. Based on IHA tests performed on ESL2 and SL2 antigens, sensitivity and specificity rates were 91% and 85%, and 82% and 96%, respectively. Based on the IHA results, using Sl3 and ESL3 antigens, the sensitivity and specificity were 86% and 100%, and 23% and 92%, respectively. A total of 206 sera samples from goats in Behbahan (Khuzestan) were tested for serodiagnosis of O. ovis infestations by IHA using ESL2 and ESL3 antigens. Results of IHA using ESL2 and ESL3 showed seroprevalences of O.ovis infestation in the animals at 59.7% and 43.2% respectively. According to the results of this study, IHA with ESL2 and ESL3 antigens can be used with indirect hemagglutination as a rapid, inexpensive detection method, especially in goats for treatment and control, minimizing their economic losses, as well as in humans for reducing myiasis.

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References

Abo-Shehada, M. N., Batainah, T., Abuharfeil, N., & Torgerson, P. R. (2003). Oestrus ovis larval myiasis among goats in northern Jordan. Preventive Veterinary Medicine, 59(1-2), 13-19.
Ahaduzzaman, M. (2019). The global and regional prevalence of oestrosis in sheep and goats: a systematic review of articles and meta-analysis. Parasites & vectors, 12(1), 1-17.
Akhtar, M., & Ishaq, H.M. (2006). Effect of coccidiosis on the humoral response of chickens vaccinated against hydropericardium syndrome. Online Journal of Veterinary Research, 10(1), 1-6.
Alborzi, A. R., Sharifi, H., Ghorbanpoor, M., & Pourmahdi Borujeni, M. (2018). Seroprevalence of Oestrus ovis infection in sheep in Southwest of Iran. Iranian Journal of Ruminants Health Research, 3(1), 35-46.
Alborzi, A., Jolodar, A., Bagherian pour, E., & Shapouri, M. S. A. (2014). Isolation and identification of excretory-secretory and somatic antigens from the Oestrus ovis larvae by SDS-PAGE and immunoblotting. In Veterinary Research Forum, 5(4), 307 - 311.
Alborzi, A.R, Ghorbanpour Najafabadi, M., & Yoosefvand, Z. (2021). Evaluation of an in-house enzyme linked immuonosorbent assay (ELISA) for detection of anti-Oestrus ovis antibodies in sheep. Iranian Journal of Veterinary Clinical Sciences, 14(2): 67-76.
Alcaide, M., Reina, D., Frontera, E., & Navarrete, I. (2005). Analysis of larval antigens of Oestrus ovis for the diagnosis of oestrosis by enzyme‐linked immunosorbent assay. Medical and veterinary entomology, 19(2), 151-157.
Angulo-Valadez, C. E., Cepeda-Palacios, R., Ascencio, F., Jacquiet, P., Dorchies, P., & Ramírez-Orduña, J. M. (2009). Relationships of systemic IgG antibody response and lesions caused by Oestrus ovis larvae (Diptera: Oestridae) in infected goats. REDVET. Revista Electrónica de Veterinaria, 10(11), 1-13.
Bahrami, A. M., & Bahrami, A. (2006). Immune response of chicken to an experimental sonicated coccidia oocyst vaccine. Archives of Razi Institute, 61(1), 48-53.
Bautista-Garfias., C. R., Angulo-Conteras, R. M., & Garay-Garzon, E. (1988). Serologic diagnosis of Oestrus ovis (Diptera: Oestridae) in naturally infested sheep. Medical and Veterinary Entomology, 2(4), 351-355.
Bello, H. J. S., Lins, J. G. G., da Silva, N. M. M., de Albuquerque, A. C. A., Amarante, M. R. V., Neto, V. A. K., & Amarante, A. F. (2022). Diagnosis of Oestrus ovis infestation in sheep by PCR and enzyme-linked immunosorbent assay. Veterinary Parasitology, 310, 109789.
Biu, A. A., & Nwosu, C. O. (1999). Incidence of Oestrus ovis infestation in Borno-White Sahel goats in the semi-arid zone of Nigeria. Veterinary research, 30(1), 109-112.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72(1-2), 248-254.
Budiono, N. G., Murtini, S., Satrija, F., Ridwan, Y., & Handharyani, E. (2020). Humoral responses to Schistosoma japonicum soluble egg antigens in domestic animals in Lindu Subdistrict, Central Sulawesi Province, Indonesia. International Journal of One Health, 6, 99-108.
Cepeda-Palacios, R., Frugère, S., & Dorchies, P. (2000). Expected effects of reducing Oestrus ovis L. mature larval weight on adult populations. Veterinary Parasitology, 90(3), 239-246.
Cornelissen, J. B. W. J., De Leeuw, W. A., & Van der Heijden, P. J. (1992). Comparison of an indirect haemagglutination assay and an ELISA for diagnosing Fasciola hepatica in experimentally and naturally infected sheep. Veterinary Quarterly, 14(4), 152-156.
Dehghani, R., Sedaghat, M. M., Esmaeli, N., & Ghasemi, A. (2012). Myiasis among slaughtered animals in Kashan, Iran: descriptive a veterinary entomological problem in the tropics. Iranian Journal of Veterinary Science and Technology, 4(1), 19-28.
Dhanalakshmi, S., Meenachi, C., & Parija, S. C. (2016). Indirect haemagglutination test in comparison with ELISA for detection of antibodies against invasive amoebiasis. Journal of Clinical and Diagnostic Research: JCDR, 10(8), 5-8.
Gathuma, J. M., & Waiyaki, P. G. (1980). Evaluation of the indirect haemagglutination test (IHA) in diagnosis of Taenia saginata cysticercosis (Cysticercus bovis) infection in cattle. Bulletin of Animal Health and Production in Africa, 28(3), 173-189.
Goddard, P., Bates, P., & Webster, K. A. (1999). Evaluation of a direct ELISA for the serodiagnosis of Oestrus ovis infections in sheep. Veterinary record, 144(18), 497-501.
Hay Frank, C., & Westwood Olwyn, M. R. (2002). Practical immunology (4th Edition) Oxford: Blackwell scientific publications. PP, 102-108.
Krupp, I. M. (1974). Haemagglutination test for the detection of antibodies specific for Ascaris and Toxocara antigens in patients with suspected visceral larva migrans. American Journal of Tropical Medicine and Hygiene, 23(3), 378-384.
Moţ, D. (2012). The Antigenic structure characterization of Oestrus ovis larvae. Animal Science and Biotechnology, 45(1), 314-318.
Pirouz, M. S., Tirgari, S., & AghaMohammadi, A. (1977). A case of ophthalmomyiasis in man by Oestrus ovis Linncacus in Tehran (Insecta Diptcra, Oestridae). Acta Medica Iranica, 19-26.
Pupić-Bakrač, A., Pupić-Bakrač, J., Škara Kolega, M., & Beck, R. (2020). Human ophthalmomyiasis caused by Oestrus ovis—first report from Croatia and review on cases from Mediterranean countries. Parasitology research, 119(3), 783-793.
Shoorijeh, S. J., Negahban, S., Tamadon, A., & Behzadi, M. A. (2009 a). Prevalence and intensity of Oestrus ovis in sheep of Shiraz, southern Iran. Tropical Animal Health and Production, 41(7), 1259-1262.
Shoorijeh, J. S., Tamadon, A., Negahban, S. H., & Behzadi, M. (2011b). Prevalence of Oestrus ovis in goats of Shiraz, southern Iran. Veterinarski arhiv, 81(1), 43-49.
Tabouret, G., Prevot, F., Bergeaud, J. P., Dorchies, P., & Jacquiet, P. (2001). Oestrus ovis (Diptera: Oestridae): sheep humoral immune response to purified excreted/secreted salivary gland 28 kDa antigen complex from second and third instar larvae. Veterinary Parasitology, 101(1), 53-66.
Tavassoli, M., Tajik, H., Malekifard, F., Soleimanzadeh, A., & Mardani, K. (2012). Seasonal infestation of Oestrus ovis larvae in slaughtered sheep in Urmia, Iran. Iranian Veterinary Journal, 7(4): 73-78.
Trevethan, R. (2017). Sensitivity, specificity, and predictive values: foundations, pliabilities, and pitfalls in research and practice. Frontiers in public health, 5, 307.
Vatankhah, A., Assmar, M., Shokrgozar, M. A., Hoseini, S. T., & Rastaghi, A. E. (2004). Introduction of an indirect haemagglutination test as a rapid diagnostic method in comparison with elisa using antigen b for diagnosis of human hydatid disease. Iranian Journal of Public Health, 33(4), 16-25.
Zumpt, F. (1965). Myiasis in man and animals in the Old World. A textbook for physicians, veterinarians and zoologists. Myiasis in Man and Animals in the Old World. A Textbook for Physicians, Veterinarians and Zoologists.PP, 267-270.

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