Effects of in ovo injection of nanocurcumin on intestinal development and serum parameters in chicken embryo

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

2 Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

Abstract

    The objective of the present study was to investigate the effects of in ovo injection of nanocurcumin on intestinal histomorphology and some serum components of the hatched chicks. The chicken eggs were injected with 1, 10, 100, 1000, and 10000 ppm of nanocurcumin, and the other two groups were used as control and injected control (saline). At the end of the experiment, blood and tissue samples were collected for biochemical and histological examination. For histological examination, small intestine was sampled and routine histological methods containing fixation, dehydration, clearing and paraffin embedding were used. Sections were stained with hematoxylin & eosin for light microscopy evaluation. Blood biochemical parameters were analyzed by an automatic analyzer. Results indicated that in ovo injection of saline significantly reduced albumin, globulin, total protein and AST (aspartat aminotransferase) compared to the control group, so that injection of nanocurcumin corrected the reduction of these factors. Uric acid was increased by injection of 1, 10 and 100 ppm nanocurcumin compared to the other groups. Blood glucose levels decreased slightly with increasing the dose of nanocurcumin, and in the 1000 ppm nanocurcumin group, the levels were significantly lower than those of the control. In ovo injection of 1000 ppm nanocurcumin significantly increased the villi height and villus height/crypt depth ratio compared to the saline and 1 ppm nanocurcumin groups. Although the primary purpose of this study was to evaluate the effects of in ovo administration of nanocurcumin, it was demonstrated that the use of saline in the in ovo injection was inappropriate. In conclusion, in ovo injection of nanocurcumin corrected saline-altered serum factors, lowered blood sugar, and increased the intestinal absorption surface.

Keywords

Main Subjects

References

Aggarwal, B.B. & Harikumar, K.B. (2009). Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. The international journal of biochemistry & cell biology, 41(1), 40-59.
Aggarwal, B.B., Sundaram, C., Malani, N. & Ichikawa, H. (2007). Curcumin: the Indian solid gold. Advances in Experimental Medicine and Biology, 595, 1-75.
Akbarian, A., Golian, A., Kermanshahi, H., Gilani, A. & Moradi, S. (2012). Influence of turmeric rhizome and black pepper on blood constituents and performance of broiler chickens. African Journal of Biotechnology, 11(34), 8606-8611.
Arshami, J., Pilevar, M., Azghadi, M.A., Raji, A.R. (2013). Hypolipidemic and antioxidative effects of curcumin on blood parameters, humoral immunity, and jejunum histology in Hy-line hens. Avicenna journal of phytomedicine, 3(2), 178.
Awad, W., Ghareeb, K. & Böhm, J. (2008). Intestinal structure and function of broiler chickens on diets supplemented with a synbiotic containing Enterococcus faecium and oligosaccharides. International Journal of Molecular Sciences, 9(11), 2205-2216.
Chen, W., Wang, R., Wan, H.F., Xiong, X.L., Peng, P. & Peng, J. (2009). Influence of in ovo injection of glutamine and carbohydrates on digestive organs and pectoralis muscle mass in the duck. British Poultry Science, 50(4), 436-442.
El-Kholy, M.S. (2013). Physiological studies on Japanese quail as affected by some nutritional treatments (PhD Thesis). Zagazig University, Egypt.
El-Kholy, M.S., Ibrahim, Z.A.E.G., El-Mekkawy. M.M.  & Alagawany, M. (2019). Influence of in ovo administration of some water-soluble vitamins on hatchability traits, growth, carcass traits and blood chemistry of Japanese quails. Annals of Animal Science, 19(1), 97-111.
Elwan, H.A., Elnesr, S.S., Xu, Q., Xie, C., Dong, X. & Zou, X. (2019). Effects of in ovo methionine-cysteine injection on embryonic development, antioxidant status, IGF-I and TLR4 gene expression, and jejunum histomorphometry in newly hatched broiler chicks exposed to heat stress during incubation. Animals, 9(1), 25.
Emadi, M. & Kermanshahi, H. (2007). Effect of turmeric rhizome powder on the activity of some blood enzymes in broiler chickens. International Journal of Poultry Science, 6(1), 48-51.
Ferket, P., De Oliveira, J., Ghane, A. & Uni, Z. (2005). Effect of in ovo feeding solution osmolality on hatching turkeys. Poultry Science, 84, 118-119.
Foye, O.T., Ferket, P.R.& Uni, Z. (2007). The effects of in ovo feeding arginine, β-hydroxy-β-methyl-butyrate, and protein on jejunal digestive and absorptive activity in embryonic and neonatal turkey poults. Poultry Science, 86(11), 2343-2349.
Gholami-Ahangaran, M., Rangsaz, N. & Azizi, S. (2016). Evaluation of turmeric (Curcuma longa) effect on biochemical and pathological parameters of liver and kidney in chicken aflatoxicosis. Pharmaceutical biology, 54(5), 780-787.
Ghorbani, Z., Hekmatdoost, A. & Mirmiran, P. (2014). Anti-hyperglycemic and insulin sensitizer effects of turmeric and its principle constituent curcumin. International journal of endocrinology and metabolism, 12(4), e18081.
Goel, A., Kunnumakkara, A.B. & Aggarwal, B.B. (2008). Curcumin as “Curecumin”: from kitchen to clinic. Biochemical pharmacology, 75(4), 787-809.
Halliwell, B. (2000). The antioxidant paradox. The Lancet, 355(9210), 1179-1180.
Haider Iqbal, D. & Singh, D. (2019). Antioxidants: A Brief Review. South Asian Research Journal of Medical Sciences, 1(2), 36-39.
Heidary, M., Hassanabadi, A. & Mohebalian, H. (2020). Effects of in ovo injection of nanocurcumin and vitamin E on antioxidant status, immune responses, intestinal morphology and growth performance of broiler chickens exposed to heat stress. Journal of Livestock Science and Technologies, 8(1), 17-27.
Liu, M., Lu, Y., Gao, P., Xie, X., Li, D., Yu, D. & Yu, M. (2020). Effect of curcumin on laying performance, egg quality, endocrine hormones, and immune activity in heat-stressed hens. Poultry Science, 99(4), 2196-2202.
Ma, Z., Shayeganpour, A., Brocks, D.R., Lavasanifar, A. & Samuel, J. (2007). High‐performance liquid chromatography analysis of curcumin in rat plasma: application to pharmacokinetics of polymeric micellar formulation of curcumin. Biomedical Chromatography, 21(5), 546-552.
Malheiros, R.D., Ferket, P.P. & Goncalves, F.M. (2012). Oxidative stress protection of embryos by “In ovo” supplementation. In XXIV World’s Poultry Congress Salvador, Bahia, Brazil. 
Mendes, T.S., Novais, E.A., Badaró, E., de Oliveira Dias, J.R., Kniggendorf, V., Lima-Filho, A.A.S. & Rodrigues, E.B. (2020). Antiangiogenic effect of intravitreal curcumin in experimental model of proliferative retinopathy. Acta Ophthalmologica, 98(1), e132-e133.
McGruder, B.M., Zhai, W., Keralapurath, M.M., Bennett, L.W., Gerard, P.D. & Peebles, E.D. (2011). Effects of in ovo injection of electrolyte solutions on the pre-and post-hatch physiological characteristics of broilers. Poultry Science, 90(5), 1058-1066.
Moniruzzaman, M. & Min, T. (2020). Curcumin, curcumin nanoparticles and curcumin nanospheres: A review on their pharmacodynamics based on monogastric farm animal, poultry and fish nutrition. Pharmaceutics, 12(5), 447.
Montagne, L., Pluske, J.R. & Hampson, D.J. (2003). A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Animal feed science and technology, 108(1-4), 95-117.
Na, L.X., Li, Y., Pan, H.Z., Zhou, X.L., Sun, D.J., Meng, M. & Sun, C.H. (2013). Curcuminoids exert glucose‐lowering effect in type 2 diabetes by decreasing serum free fatty acids: A double‐blind, placebo‐controlled trial. Molecular nutrition and food research, 57(9), 1569-1577.
Nassar, F.S. (2018). Poultry as an experimental animal model in medical research and pharmaceutical industry. Biomed Journal, 2(4), 1-4.
Patel, S., Jana, S., Chetty, R., Thakore, S., Singh, M. & Devkar, R. (2018). TiO 2 nanoparticles induce omphalocele in chicken embryo by disrupting Wnt signaling pathway. Scientific Reports, 8(1), 1-11.
Rahardja, D.P., Hakim, M.R., Yusuf, M. & Lestari, V.S. (2019). Hatching and post-hatching performances of Indonesian native chicken eggs infused saline solution. In IOP Conference Series: Earth and Environmental Science, 247(1), 012014.
Rahimi, H.R., Mohammadpour, A.H., Dastani, M., Jaafari, M.R., Abnous, K., Mobarhan, M.G. & Oskuee, R.K. (2016). The effect of nano-curcumin on HbA1c, fasting blood glucose, and lipid profile in diabetic subjects: a randomized clinical trial. Avicenna journal of phytomedicine, 6(5), 567.
Rahmani, M., Golian, A., Kermanshahi, H. & Bassami M.R. (2018). Effects of curcumin or nanocurcumin on blood biochemical parameters, intestinal morphology and microbial population of broiler chickens reared under normal and cold stress conditions. Journal of applied animal research, 46(1), 200-209.
Rajput, N., Muhammah, N., Yan, R., Zhong, X. & Wang, T. (2012). Effect of dietary supplementation of curcumin on growth performance, intestinal morphology and nutrients utilization of broiler chicks. The Journal of Poultry Science, 50, 44–52.
Reda, F.M., El-Saadony, M.T., Elnesr, S.S., Alagawany, M. & Tufarelli, V. (2020). Effect of dietary supplementation of biological curcumin nanoparticles on growth and carcass traits, antioxidant status, immunity and caecal microbiota of Japanese quails. Animals, 10(5), 754.
Sayrafi, R., Shahrooz, R., Soltanalinejad, F. & Rahimi, S. (2011). Histomorphometrical study of the prebiotic effects on intestine morphology and growth performance of broiler chickens. Veterinary Research Forum, 2(1), 45-51.
Sayrafi, R., Hosseini, S.M. & Ahmadi, M.A. (2017). The protective effects of nanocurcumin on liver toxicity induced by salinomycin in broiler chickens. Revue de Médecine Vétérinaire, 168(7/9), 136-142.
Selim, S.A., Gaafar, K.M. & El-ballal, S.S. (2012). Influence of in-ovo administration with vitamin E and ascorbic acid on the performance of Muscovy ducks. Emirates Journal of Food and Agriculture, 24(3), 264-271.
Selvi, N.M.K., Sridhar, M.G., Swaminathan, R.P. & Sripradha, R. (2015). Efficacy of turmeric as adjuvant therapy in type 2 diabetic patients. Indian Journal of Clinical Biochemistry, 30(2): 180-186.
Shehzad, A., Wahid, F. & Lee, Y.S. (2010). Curcumin in cancer chemoprevention: molecular targets, pharmacokinetics, bioavailability, and clinical trials. Archiv der Pharmazie, 343(9), 489-499.
Steiner, T. (2009). Phytogenics in animal nutrition. Nottingham University Press. Nattingham. UK.
Suvanated, C., Kijparkorn, S. & Angkanaporn, K. (2003) Effect of turmeric (Curcuma longa linn.) as an antioxidant on immune status and growth performances of stressed broilers (PhD Thesis). Chulalongkorn University, Bangkok, Thailand.
Sieo, C.C., Abdullah, N., Tan, W.S. & Ho, Y.W. (2005). Influence of β-glucanase-producing Lactobacillus strains on intestinal characteristics and feed passage rate of broiler chickens. Poultry Science, 84(5): 734-741.
Uni, Z., Yadgary, L.& Yair, R. (2012). Nutritional limitations during poultry embryonic development. Journal of Applied Poultry Research, 21(1), 175-184.
 Urso, U.R.A., Dahlke, F., Maiorka, A., Bueno, I.J.M., Schneider, A.F., Surek, D. & Rocha, C. (2015). Vitamin E and selenium in broiler breeder diets: Effect on live performance, hatching process, and chick quality. Poultry Science, 94(5), 976-983.
Yang, Q.Q., Cheng, L.Z., Zhang, T., Yaron, S., Jiang, H.X., Sui, Z.Q. & Corke, H. (2020). Phenolic profiles, antioxidant, and ant proliferative activities of turmeric (Curcuma longa). Industrial Crops and Products, 152, 112561.
Yoshiyama, Y., Sugiyama, T. & Kanke, M. (2005). Experimental diabetes model in chick embryos treated with streptozotocin. Biological and Pharmaceutical Bulletin, 28(10), 1986-1988.

Files

Share

How to cite

Statistics