Evaluation of the effect of royal jelly on hepatotoxicity (histopathological and biochemical changes) induced by arsenic trioxide in male rats according to the treatment dose of acute promyelocytic leukemia

Document Type : Research Paper

Authors

1 Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Student of Pathology Course, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Professor, Department of Pharmacology, Faculty of Medical Sciences, Babol University, Babol, Iran

4 Associate Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

     Arsenic trioxide (ATO) is used in treatment of acute promyelocytic leukemia (APL). One of the major side effects of this drug is liver toxicity. Different doses of this drug have been used in different studies. Based on the ATO treatment protocol in APL patients (0.15 mg / kg) and the dose conversion formula between humans and laboratory animals introduced by the FDA, a dose of 1 mg / kg was selected. Royal jelly has attracted increasing attention in recent years given its high antioxidants capacity. This study aimed to evaluate the effect of royal jelly on histopathologic and biochemical changes of liver based on arsenic trioxide-induced hepatotoxicity in 8 groups of 5 rats. In groups one, two and three respectively, normal saline, royal jelly and arsenic trioxide; in groups four, five and six, different doses of royal jelly along with fixed dose of arsenic trioxide; in group seven arsenic trioxide along with vitamin E; in group eight arsenic trioxide alonge with vitamin E and royal jelly were given orally for 30 days. According to the results, the liver was normal in the normal salin group and royal jelly receiving groups, while it had pathological changes as hyperemia and cellular swelling (reversible changes) in other groups. The severity of these changes was reduced in royal jelly receiving groups. There was no significant change in the liver enzymes activity in all study groups.

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References

Anbara, H., Shahrooz, R., Malekinejad, H. & Saadati, S. (2016). nvestigating the Antioxidant Properties of Royal Jelly and Vitamin C on Enzymes, Histomorphometric and Liver Cells Apoptosis in Mice Suffering Hemolytic Anemia. Journal of Fasa University of Medical Sciences, 6(2).
Asgari, M., Asle- Rusta, M. & Sofi-Abadi, M. (2016). Effect of royal jelly on blood glucose and lipids in in streptozotocin-diabetic rats. Journal of Arak University of Medical Sciences, 20(5): 48-56.
Chowdhury, S.,  Islam, S., Akter, R., Khaleda, L., Rahman, Z. & Al-Fforkan, M. (2016). A study on the effect of arsenic on tissue histology and its deposition pattern in various organs of wistar albino rat. European journal of pharmaceutical and medical research, 3(5): 580-587.
Islam, K., Haque, A., Karim, R., Fajol, A., Hossain, E., Abdus S.K. & … (2011). Dose- response relationship between arsenic exposure and the serum enzymes for liver function tests in the individuals exposed to arsenic: a cross sectional study in bangladesh. Environ Health, 10: 64.
Jomova, K., Jenisova, Z., feszterova, M., Baros, S., Liska, J., Hudecova, D., & . . . (2011). Arsenic : toxicity oxidative stress and human disease. Journal of applied toxicology, 31, 95-107.
Singh, K., Manish, D., Shailendra, S., Yadav, S., Sharma, P. & Khattri, S. (2014). Arcenic- induced hepatic toxicity and its attenuation by fruit extract of emblica officinalis (Amla) in mice. India journal clinical biochemists, 29(1): 29-37.
Kamakura, M. (2011). Royalactin induces queen differentiation in honeybees. Nature, vol. 473, no. 7348, pp. 478–483.
Khodabande, Zh., Negati, V., Nagafi, Gh., Shalizar Jalali, A. & Rahmani, F. (2018). Protective Effect of Royal Jelly on Liver Tissue in Nicotine-treated Adult Female Rats. Journal of Neyshabur University of Medical Sciences, 5(4): 22-31.
Njafzadeh Varzi, H., Khaksar Mahabadi, M. & Haji, A. (2015). Comparative Study of the Effects of Sodium Arsenite and Nanoparticles ‎of Sodium Arsenite on the Apparent and Skeletal Malformations in Rat ‎Embryos. Journal of Babol University of Medical Sciences, 17 (10) :60-66 .
Nejati, V., Zahmatkesh, E. & Babaei, M. (2016). Protective effects of royal jelly on oxymetholone- induced liver injury in mice. Iranian biomedical journal, 20(4): 229-234.
Noman, A.S.M., Dilruba, S., Mohanto, N.C., Rahman, L., Khatun, Z., Riad, W. & … (2015). Arsenic indced histological alteration in various organs of mice. Journal of cytology histology, 6: 3-323.
Obinaju, E.B. (2009). Mechnisms of arsenic toxicity and carcinogenesis. Africa jurnal of biochemistry research, 3(5): 232-237.
Reagan-Shaw, S., Nihal, M. & Ahmad, N. (2008). Dose translation from animal to human studies revisited. The FASEB Journal, 22(3): 659-661.
Shirazi, M., Kordyazdi, R., Shahinfard, N. & Nikokar, M. (2013). Dose royal jelly affect tumor cells. Jouarnl of herbmed pharmacology, 2(2): 45-48.
Soleimani Mehranjani, M., Shariat-zadeh, M.A., Maleki, P. & Mahmoodi, M. (2007). Quantitative study of the histopathological effects of sodium arsenite on kidney stracture in rats. Journal of Arak University of Medical Sciences, 10(4): 1-17.
Tanju, S. & Madhuri, D. (2013). Arsenic induced oxidative stress, hemato-biochemical and histological changes in liver and protective effect of moringa leaf powder and ascorbic acid in broiler chicken. Jornal of chemical and pharmaceutical research, 5(2): 112-116.
Yousofvand, N. & Fahim, M. (2015). Effects of chronic exposure to sodium arsenate on kidney of rats. Iranian Journal of Toxicolog, 9(30):  1402-1406.
Zubair, M., Ahmad, M., Kashif Saleemi, M., Tehseen Gul, S., Jozeph Martyinuk, Ch., Ullah, Q. & Umer, S. (2020). Sodium arsenite toxicity on hematology indices and reproductive parameters in Teddy goat bucks and their amelioration with vitamin C. Environmental Science and Pollution Research, 27: 15223-15232.

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