Histomorphological study of liver, spleen and pancreas in four cichlid species

Document Type : Research Paper

Authors

1 Professor, Department Livestock Animal, Poultry and Fish Health, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 PhD Graduated of Fish Health, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 PhD Student of Fish Health, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

4 Assistant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

    The cichlidae family has more than 1300 species which in turn have great species morphologic differences. In this study, histology of liver, spleen and pancreas of four cichlid species (green terror cichlid, Oscar, giraffe cichlid and Texas cichlid) were compared. For this purpose, four apparently normal fish specimens from each species were selected after killing of which, tissue samples were taken. The sections stained with two methods (Haematoxillin- Eosin and Periodic acid Schiff). The results showed that all liver sections contained pancreas tissues around the liver blood vessels. Liver cells size in two species (green terror cichlid and giraffe cichlid) was significantly bigger than the two other species. The liver cell to pancreatic cell ratios also was significantly higher in two species. The liver size in giraffe cichlid, was even significantly bigger than the green terror cichlid. Exocrine pancreatic cell size in all 4 species showed no significant difference. The spleen in four fish species showed different degrees of amount and intensity in melanomacrophage centers. In PAS stained sections, only the pancreatic tissues showed more PAS positive reaction that may be due to the more glycogen content. The liver showed a weak PAS reaction. The bigger size formation of liver cells in two species can be due to feeding regime or simply a species specific trait. Yet more investigation is still needed to determine the importance of these findings.

Keywords

Main Subjects

References

Akoul, M. A., & AL-Jowari, S. A. K. (2019). Comparative anatomical and histological study of some organs in two fish species Cyprinus carpio Linnaeus, 1758 and Mesopotamichthys sharpeyi (Günther, 1874) (Cypriniformes, Cyprinidae). Bulletin of the Iraq Natural History Museum, 15(4): 425-441.
AL-Abdulhadi, H. A. (2005). Some comparative histological studies on alimentary tract of tilapia fish (Tilapia spilurus) and sea bream (Mylio cuvieri). Egyptian Journal of Aquatic Research, 31(1): 387-397.
Ghosh, P. K. & Chakrabarti, P. (2016) Comparative studies on histology and histochemistry of pancreas between Labeo calbasu (Hamilton, 1822) and Mystus gulio (Hamilton, 1822).  Iranian Journal of Ichthyology, 3(4): 251–265. 
Ghosh, S., Sinha, A., & Sahu, C. (2008). Dietary probiotic supplementation in growth and health of live‐bearing ornamental fishes. Aquaculture Nutrition, 14(4): 289-299.
Ismayil, S., & Joseph, A. (2021). Histology of the freshwater fish Catla catla contaminated with sublethal levels of imidacloprid 17.8% in paddy field. International Research Journal of Modernization in Engineering Technology and Science, 3 (3): 1848-1856.
Maan, M. E. & Sefc, K. M. (2013). Colour variation in cichlid fish: developmental mechanisms, selective pressures and evolutionary consequences. Seminars in Cell and Developmental Biology, 24, 6-7, 516-528.
Mohammadiazarm, H., Maniat, M., Ghorbanijezeh, K., & Ghotbeddin, N. (2021). Effects of spirulina powder (Spirulina platensis) as a dietary additive on Oscar fish, Astronotus ocellatus: Assessing growth performance, body composition, digestive enzyme activity, immune‐biochemical parameters, blood indices and total pigmentation. Aquaculture Nutrition. 27: 252-260.
Mokhtar, D. M. (2015). Histological, histochemical and ultrastructural characterization of the pancreas of the grass carp (Ctenopharyngodon idella). European Journal of Anatomy, 19(2): 145-153.
Press, C. M., & Evensen, Ø. (1999). The morphology of the immune system in teleost fishes. Fish and shellfish immunology, 9(4): 309-318.
Manera, M., Castaldelli, G., Fano, E.A. & Giari, L. (2021). Perfluorooctanoic acid-induced cellular and subcellular alterations in fish hepatocytes. Environmental Toxicology and Pharmacology, 81: 103548.
Nath, S., Matozzo, V., Bhandari, D. & Faggio, C. (2019). Growth and liver histology of Channa punctatus exposed to a common biofertilizer. Natural Product Research, 13(11): 1591-1598.
Riehl, R. & Baensch, H.A. (1996). Aquarien Atlas, Band 1. 10th edition. Mergus Verlag GBH, Melle, Germany. 992 p.
Saemi-Komsari, M., Salehi, M., Mansouri-Chorehi, M., Eagderi, S., & Mousavi-Sabet, H. (2018). Developmental morphology and growth patterns of laboratory-reared giraffe cichlid, Nimbochromis venustus Boulenger, 1908. International Journal of Aquatic Biology, 6(3): 170-178.
Sales, C. F., Silva, R. F., Amaral, M. G., Domingos, F. F., Ribeiro, R. I., Thomé, R. G., & Santos, H. B. (2017). Comparative histology in the liver and spleen of three species of freshwater teleost. Neotropical Ichthyology, 15(1): 1-11.
Sarvestani, F. S. (2017). Comparative histology of liver in Pangusius Pangusius (herbivore fish) and Notopetrus Chitals (carnivore fish). International Journal of Pharmaceutical Research and Allied Sciences, 6(4): 28-31.
Snoeks J., & Manuel J. (2004). The genus Nimbochromis Eccles & Trewavas, 1989. In: J. Snoeks (ed.). The cichlid diversity of Lake Malawi/Nyasa/Niassa: identification, distribution and taxonomy. Cichlid Press, El Paso, USA. pp: 57-85.
Steinel, N.C. & Bolnick, D.I. (2017) Melanomacrophage centers as a histological indicator of immune function in fish and other poikilotherms. Frontiers in Immunology. 8: 827- 835.
Taddese, F., Huh, M. D., Bai, S. C., & Vijverberg, J. (2014). Histological changes of liver in overfed young nile tilapia. Journal of Fisheries and Aquatic Science, 9(2), 63-74.
Vicentini, C. A., Franceschini-Vicentini, I. B., Bombonato, M. T. S., Bertolucci, B., Lima, S. G., & Santos, A. S. (2005). Morphological study of the liver in the teleost Oreochromis niloticus. International Journal of Morphology, 23(3): 211-21.
Wijkmark, N., Kullander, S. O., & Barriga Salazar, R. E. (2012). Andinoacara blombergi, a new species from the río Esmeraldas basin in Ecuador and a review of A. rivulatus (Teleostei: Cichlidae). Ichthyological Exploration of Freshwaters, 23(2): 117-127.
Zihler, F. (1981). Gross morphology and configuration of digestive tracts of Cichlidae (Teleostei, Perciformes): phylogenetic and functional, significance. Netherlands Journal of Zoology, 32(4): 544-571.

Files

Share

How to cite

Statistics