Comparative study of nanostructured effect of alumina-graphite with Autogenous rib cartilage in the repair of bone defects in dogs

Document Type : Research Paper

Authors

1 DVM Graduated, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran

2 Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran

3 Assistant Professor, Faculty of Applied Chemistry, Kashan University, Kashan, Iran

4 Associated Professor, Department of Pathobiology, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran

5 Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Shiraz, Shiraz, Iran

Abstract

    Bone tissue has attractive structural features, especially due to its hybrid bone structure, which is a combination of hydroxyapatite, collagen, and small amounts of proteoglycans, non-collagenous proteins and water. The chemical structure of the material used is a mixture of alumina powder and graphite. Alumina is a non-acidic and non-base mineral structure that is classified in terms of porosity as a Nano-porous material. In some of its structures, half the volume belongs to the porous spaces. 15 dogs were randomly divided into three groups of control, autogenous cartilage recipient and alumina graft recipient group. By removing the skin, connective tissue, and muscles, the humerus was exposed and a 2 cm long and 1 cm wide incision was made in the humerus body and the alumina-graffiti was placed in the defect, the other group autogenous cartilage resected from rib arch was inserted in the created defect. In control group did not place any material in the created defect. There was a significant difference between control groups, autogenous cartilage group and alumina grafit in bone repair criteria. Using alumina graphite can cause bone healing faster than autogenous rib cartilage.

Keywords


Abu-Amer, Y.; Darwech, I. and Clohisy, J.C. (2007). Aseptic loosening of total joint replacements: mechanisms underlying osteolysis and potential therapies. Arthritis research and therapy, 9(1):S6.
Arrington, E.D.; Smith, W.J.; Chambers, H.G.; Bucknell, A.L. and Davino, N.A. (1996). Complications of iliac crest bone graft harvesting. Clinical Orthopaedics and Related Research®, 329: 300-309.
Bardsley, K.; Kwarciak, A.; Freeman, C.; Brook, I.; Hatton, P. and Crawford, A. (2017). Repair of bone defects in vivo using tissue engineered hypertrophic cartilage grafts produced from nasal chondrocytes. Biomaterials, 112: 313-323.
Bauer, T.W. and Schils, J. (1999). The pathology of total joint arthroplasty. Skeletal radiology, 28(9):483-497.
Bigham-Sadegh, A. and Oryan, A. (2015). Selection of animal models for pre-clinical strategies in evaluating the fracture healing, bone graft substitutes and bone tissue regeneration and engineering. Connective tissue research, (0): 1-20.
Bigham‐Sadegh, A. and Oryan, A. (2015). Basic concepts regarding fracture healing and the current options and future directions in managing bone fractures. International wound journal, 12(3): 238-247.
Bostrom, M. P.; Saleh, K.J. and Einhorn, T.A. (1999). Osteoinductive growth factors in preclinical fracture and long bone defects models. Orthopedic Clinics, 30(4): 647-658.
Damien, C.J. and Parsons, J.R. (1991). Bone graft and bone graft substitutes: a review of current technology and applications. Journal of Applied Biomaterials, 2(3) :187-208
Den Boer, F.C.; Wippermann, B. W.; Blokhuis, T. J.; Patka, P.; Bakker, F. C. and Haarman, H.J.T.M. (2003). Healing of segmental bone defects with granular porous hydroxyapatite augmented with recombinant human osteogenic protein‐I or autologous bone marrow. Journal of orthopaedic research, 21(3): 521-528.
Hesaraki, S. (2016). Feasibility of alumina and alumina-silica nanoparticles to fabricate strengthened betatricalcium phosphate scaffold with improved biological responses. Ceramics International,42:7593-7604
Junqueira, L.C. and Carneiro, J. (2005). Basic histology text and atlas London: McGraw Hill, 96:39-46
Kurşun, A.; Bayraktar, E. and Enginsoy, H-M. (2016). Experimental and Numerical Study of Alumina Reinforced Aluminium Matrix Composites: Processing, Microstructural Aspects and Properties. Composites Part B: Engineering, B(90): 302-314.
Montufar-Solis, D.; Nguyen, H.; Nguyen, H.; Horn, W.; Cody, D. and Duke, P. (2004). Using cartilage to repair bone: an alternative approach in tissue engineering. Annals of biomedical engineering, 32(3): 504-509.
Özdal-Kurt, F.; Tuğlu, I.; Vatansever, H.; Tong, S. and Deliloğlu-Gürhan, S. (2015). The effect of autologous bone marrow stromal cells differentiated on scaffolds for canine tibial bone reconstruction. Biotechnic & Histochemistry,90(7):516-528
Pelttari, K.; Pippenger, B.; Mumme, M.; Feliciano, S.; Scotti, C.; Mainil-Varlet, P. and Jakob, M. (2014). Adult human neural crest–derived cells for articular cartilage repair. Science translational medicine, 6(251): 119-125
Pippenger, B.E.; Ventura, M.; Pelttari, K.; Feliciano, S.; Jaquiery, C.; Scherberich, A. and Martin, I. (2015). Bone‐forming capacity of adult human nasal chondrocytes. Journal of cellular and molecular medicine, 19(6): 1390-1399.
Saifzadeh, S.; Hobbenaghi, R. and Hodi, S. (2006). Elastic cartilage grafting in canine radial fracture. Iranian Journal of Veterinary Research, 7(4): 1-7.
Sharifi, S.; Karimi, I.; Soltani, S.; Bigham-Sadegh, A. and Hosseini, F. (2017). Comparison of Autogenic Costal Cartilage with Chitosan Scaffold in Canine Humeral Defect Healing. Iranian Journal of Veterinary Surgery, 12(1): 33-39.
Tang, D.; Xu, G.; Yang, Z.; Holz, J.; Ye, X.; Cai, S. and Wang, Y. (2014). Biphasic calcium phosphate nano-composite scaffolds reinforced with bioglass provide a synthetic alternative to autografts in a canine tibiofibula defect model. Chinese medical journal, 127(7): 1334-1338.
Tawonsawatruk, T.; Hamilton, D.F. and Simpson, A.H.R. (2014). Validation of the use of radiographic fracture‐healing scores in a small animal model. Journal of orthopaedic research, 32(9): 1117-1119.
Tjelmeland, K. and Stal, S. (2000). Cartilage graft resorption: an animal model. Aesthetic Surgery Journal, 20(6): 471-476.
Xiao, Y.; Yin, Q.; Wang, L. and Bao, C. (2015). Macro-porous calcium phosphate scaffold with collagen and growth factors for periodontal bone regeneration in dogs. Ceramics International, 41(1): 995-1003.