Histopathologic Evaluation of Honey-Zinc Oxide Combination on Full-Thickness Experimental Wound Healing in Rats

Document Type : Research Paper

Authors

1 DVM Graduate, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

    Wounds are the creation of any defects and loss of connectivity of body tissues, both inside and outside the body. Researchers have always sought to find the most effective and least complicating factor to accelerate the wound healing process. The present study aimed to evaluate and compare the effectiveness of honey alone or in combination with zinc oxide on healing the experimental defect of skin full-thickness wound in rats. In this study, 48 adult male white Wistar rats were selected and randomly divided into four groups of 12 including the first group (control = C), the second group (honey = H), the third group (zinc = Z), and the fourth group (honey and zinc oxide= (ZH). A full-thickness skin defect with dimensions of 2 × 2 cm was created in the skin of the back, as well as the distance between the neck and the pelvic area. All animals received treatment appropriate to their group. Four rats from each group were prepared on histopathological samples on days 7, 14, and 21 and microscopic examination was performed. Axiovision software was used for macroscopic assessments. The highest healing rate on day 7 after wounding belonged to the Z group and the lowest to the H group. Also, the highest rate of healing on the 14th day after wounding belonged to the Z group and the lowest to the ZH group. However, no statistically significant differences were observed between the groups. On day 21 after wounding, the percentage of wound contraction in the ZH group was significantly higher than the C (p = 0.001), H (p = 0.001), and Z (p = 0.013) groups. Microscopic examination of the skin, 21 days after wounding, showed that the wound was completely covered by amplified keratinocytes in all rats in the ZH group. This was seen in two rats in the Z group and one in the C group. In all rats in the H group, the wound surface was still not completely covered and there was a scab on the wound. In the dermis of all ZH rats, regular clusters of collagens with a small number of fibroblasts and capillaries were observed. This study showed that the combination of zinc oxide and honey has a better effect on the wound healing process than other groups and accelerates the wound healing process. The combination of these two substances increases the formation of fleshy tissue and wound contraction and reduces inflammation and wound scarring.

Keywords

References

Agren, M. S., Chvapil, M., & Franzén, L. (1991). Enhancement of re-epithelialization with topical zinc oxide in porcine partial-thickness wounds. Journal of Surgical Research, 50(2), 101-105.
Agren, M. S., Ostenfeld, U., Kallehave, F., Gong, Y., Raffn, K., Crawford, M. E., Kiss, K., Friis‐Moller, A., Gluud, C. & Jorgensen, L. N. (2006). A randomized, double‐blind, placebo‐controlled multicenter trial evaluating topical zinc oxide for acute open wounds following pilonidal disease excision. Wound Repair and Regeneration, 14(5), 526-535.
 
Aksoy, B., Atakan, N., Aksoy, H. M., Tezel, G. G., Renda, N., Özkara, H. A., & Önder, E. (2010). Effectiveness of topical zinc oxide application on hypertrophic scar development in rabbits. Burns, 36(7), 1027-1035.
Al-Waili, N., Salom, K., & Al-Ghamdi, A. A. (2011). Honey for wound healing, ulcers, and burns; data supporting its use in clinical practice. The Scientific World Journal, 11, 766-787.
 
Marriage-Arcari, R. (2016). The role of Zinc in wound healing. Journal of Wound Care Canada, 14(3), 18-22.
Arslan, K., Karahan, O., Okus, A., Unlu, Y., Eryilmaz, MA., Ay, S., & Sevinç, B. (2012). Comparison of topical zinc oxide and silver sulfadiazine in burn wounds: an experimental study. Ulus Travma Acil Cerrahi Derg, 18(5): 376-383.
Cangul, IT., Gul, N.Y., Topal, A., & Yilmaz, R. (2006). Evaluation of the effects of topical tripeptide‐copper complex and zinc oxide on open‐wound healing in rabbits. Veterinary Dermatology, 17(6): 417-423.
Chaudhary, A., Bag, S., Banerjee, P., & Chatterjee, J. (2020). Wound healing efficacy of Jamun honey in diabetic mice model through reepithelialization, collagen deposition and angiogenesis. Journal of Traditional and Complementary Medicine, 10(6), 529-543.
Chen, Z., Hu, Y., Li, J., Zhang, C., Gao, F., Ma, X., Zhang, J., Fu, C., & Geng, F. (2019). A feasible biocompatible hydrogel film embedding Periplaneta americana extract for acute wound healing. International Journal of Pharmaceutics, 571: 118707.
Cui, Y., An, Y., Jin, T., Zhang, F., & He, P. (2017). Real-time monitoring of skin wound healing on nano-grooves topography using electric cell-substrate impedance sensing (ECIS). Sensors and Actuators B: Chemical, 250: 461-468.
Ehterami, A., Salehi, M., Farzamfar, S., Samadian, H., Vaez, A., Ghorbani, S., Ai, J., & Sahrapeyma, H. (2019). Chitosan/alginate hydrogels containing Alpha-tocopherol for wound healing in rat model. Journal of Drug Delivery Science and Technology, 51: 204-213.
Ghaderi, R., & Afshar, M. (2015). Topical application of honey for treatment of skin wound in mice. Iranian Journal of Medical Sciences, 29(4), 185-188.
Ghashghaii, A., Hashemnia, M., Nikousefat, Z., Zangeneh, MM., & Zangeneh, A. (2017). Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharmaceutical Sciences, 23(4): 256-263.
Gutha, Y., Pathak, JL., Zhang, W., Zhang, Y., & Jiao, X. (2017). Antibacterial and wound healing properties of chitosan/poly (vinyl alcohol)/zinc oxide beads (CS/PVA/ZnO). International journal of Biological Macromolecules, 103: 234-241.
Kogan, S., Sood, A., & Garnick, M. S. (2017). Zinc and wound healing: a review of zinc physiology and clinical applications. Wounds: a compendium of Clinical Rresearch and Practice, 29(4): 102-106.
Khoo, Y. T., Halim, A. S., Singh, K. K. B. & Mohamad, N. A. (2010). Wound contraction effects and antibacterial properties of Tualang honey on full-thickness burn wounds in rats in comparison to hydrofibre. BMC Complementary and Alternative Medicine, 10(1): 48.
Kumar, S., Lakshmanan, V. K., Raj, M., Biswas, R., Hiroshi, T., Nair, S. V., & Jayakumar, R. (2013). Evaluation of wound healing potential of β-chitin hydrogel/nano zinc oxide composite bandage. Pharmaceutical research, 30(2): 523-537.
Lansdown, A. B.; Mirastschijski, U.; Stubbs, N.; Scanlon, E. & Ågren, MS. (2007). Zinc in wound healing: theoretical, experimental, and clinical aspects. Wound Repair and Regeneration, 15(1): 2-16.
Latif, M., A.; Zaki, M. Z. M., Leng, T. M., Rahman, N. H. A., Arshad, S. A. & Hamid, A. (2015). Alocasia denudata Engler treatment enhance open wound healing activities in Wistar rat’s skin. Journal of Ethnopharmacology, 176: 258-267.
Lee, K. V., Steinhauer, N., Rennich, K., Wilson, M. E., Tarpy, D. R., Caron, D. M., Sagili, R. (2015). A national survey of managed honey bee 2013–2014 annual colony losses in the USA. Apidologie, 46(3), 292-305.
Lusby, P. E., Coombes, A., & Wilkinson, J. M. (2002). Honey: a potent agent for wound healing. Journal of WOCN, 29(6): 295-300.
Meo, S. A., Al-Asiri, S. A., Mahesar, A. L., & Ansari, M. J. (2017). Role of honey in modern medicine. Saudi Journal of Biological Sciences, 24(5): 975-978.
Oryan, A., Alemzadeh, E., & Mohammadi, AA. (2019). Application of honey as a protective material in maintaining the viability of adipose stem cells in burn wound healing: A histological, molecular and biochemical study. Tissue and Cell, 61: 89-97.
Sazegar, G., Reza, A. H. S. & Behravan, E. (2011). The effects of supplemental zinc and honey on wound healing in rats. Iranian Journal of Basic Medical Sciences, 14(4): 391.
Vaghardoost, R., Majd, S. G. M., Tebyanian, H., Babavalian, H., Malaei, L., Niazi, M., & Javdani, A. (2018). The healing effect of sesame oil, camphor and honey on second degree burn wounds in rat. World Journal of Plastic Surgery, 7(1): 67.
Vakilian, S., Jamshidi-adegani, F., Al-Shidhani, S., Anwar, M. U., Al-Harrasi, R., Al-Wahaibi, N., Qureshi, A., Alyaqoobi, S., Al-Amri, I., Al-Harrasi, A., Al-Hashmi, S. (2019). A Keratin-based biomaterial as a promising dresser for skin wound healing. Wound Medicine, 25(1), 100155.
Vasile, B. S., Oprea, O., Voicu, G., Ficai, A., Andronescu, E., Teodorescu, A., & Holban, A. (2014). Synthesis and characterization of a novel controlled release zinc oxide/gentamicin–chitosan composite with potential applications in wounds care. International Journal of Pharmaceutics, 463(2): 161-169.
Yaghoobi, R. & Kazerouni, A. (2013). Evidence for clinical use of honey in wound healing as an anti-bacterial, anti-inflammatory anti-oxidant and anti-viral agent: A review. Jundishapur Journal of natural Pharmaceutical Products, 8(3) :100-112.

Files

Share

How to cite

Statistics