توزیع آلکالین فسفاتاز و کربوهیدرات ها در مراحل اولیه رویانی بوقلمون

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته دکتری بافت شناسی، گروه علوم پایه، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران

2 استاد، گروه علوم پایه، دانشکده دامپزشکی، دانشگاه فردوسی مشهد، مشهد، ایران

3 استاد، گروه علوم پایه، دانشکده دامپزشکی، دانشگاه فردوسی مشهد و گروه پژوهشی سلول های بنیادی و طب بازساختی، پژوهشکده فناوری زیستی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

    در مطالعات تکوینی رویان، تعیین روش­های مناسب شناسایی بافت­های مختلف بسیار اهمیت دارد.  هدف از این مطالعه، تعیین نشان­گر مناسب در تکوین سیستم عصبی و سایر اندام‌ها در رویان بوقلمون بوده تا راهنمایی باشد در جهت کشف نقش آنزیم آلکالین فسفاتاز در تکوین رویان، درک بهتر عملکرد اندام پس از تولد و مطالعات روند جنینی تشکیل دستگاه عصبی.  رویان‌های بوقلمون در مراحل  (H&H)31-19، با استفاده از تکنیک‌های هیستوشیمی و ایمونوهیستوشیمی مطالعه شدند.  نتایج نشان داد که سلول‌ها تنها در طناب نخاعی، قادر به بیان نشان­گر سطحی SSEA-1 بوده و سایر اندام‌های مطالعه شده از نظر بیان این مارکر، منفی بودند.  اگر چه در مراحل (H&H)  31-19، فعالیت آلکالین فسفاتازی منفی یا بسیار ضعیف بود اما در 5/9-9 روز پس از انکوباسیون، واکنش قلیایی مثبت در بخش‌هایی از طناب نخاعی، مزونفروز، گناد، آئورت پشتی و سینوزوئید کبدی مشاهده گردید.  برش‌های رنگ­آمیزی شده با تکنیک هیستوشیمی پریودیک اسید شیف (PAS) نیز حضور گلیکوژن در قلب، مزونفروز، گناد، نوتوکورد، کندروسیت و بخش‌هایی از طناب نخاعی را تأیید کرد.  نتایج به دست آمده از تحقیق حاضر نشان می‌دهند که آنزیم آلکالین فسفاتاز و بیان SSEA-1 می‌توانند نشان­گرهای مناسبی در مطالعات تکوینی سیستم عصبی رویان باشند.  همچنین می‌توان از تکنیک‌های هیستوشیمی پریودیک اسید شیف و آلکالین فسفاتاز قلیایی نیز به منظور مطالعه دیگر اندام‌ها در رویان بوقلمون استفاده کرد.

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