Effects of different levels of commercial food supplement, Biotronic® Top3 on biochemical and immune parameters of Litopenaeus vannamei

Document Type : Research Paper

Authors

1 MSC Graduated of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran

2 Professor, Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran

3 Associate Professor, Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran

4 Expert, Abadan Office of Shrimp Development and Marine Aquatic Species, Khoozestan Fisheries Head Department, Iranian Fisheries Organization, Iran

Abstract

    The effect of different dietary levels of Biotronic® Top3 (Biomin, Austria) on hemolymph biochemical parameters, hemolymph enzymes and immune parameters of white leg shrimp (Litopenaeus vannamei) was investigated. 375 Shrimps (mean initial weight: 4.28±0.05g) collected from the commercial farm and stocked randomly in 300L polyethylene tanks in triplicates. The shrimps were fed with commercial diet (4005, Faradaneh Company) supplemented with different levels of Biotronic® Top3 (0: Control, 0.5%, 1%, 2%, and 4%) for 56 days (8 weeks). At the end of the experiment, the biochemical indices, levels of hemolymph enzymes (AST, ALT, ALP and LDH), hemolymph immune parameters (Lysozyme and Phenoloxidase), and cellular hemolymph parameters of experimental shrimps were compared. The dietary inclusion of 2% and 4% of Biotronic® Top3 led to an increase in hemolymph enzymes. Shrimp were fed with supplemented diets also had lower levels of glucose, cholesterol and triglyceride and higher levels of protein, calcium and creatinine. Addition of Biotronic® Top3 in the feed had a positive effect on the cellular and humoral immune responses of shrimps. The results of this study showed that the best performance of dietary supplementation of Biotronic® Top3 on L. vanammei was observed at level of 1%.

Keywords

References

Abdel-Fattah, S.A., El-Sanhoury, M.H., El-Mednay, N.M. & Abdel-Azeem, F. (2008). Thyroid activity, some blood constituents, organs morphology and performance of broiler chicks fed supplemental organic acids. International Journal of Poultry Science, 7, 215-222.
Arshadi, A., Yavari, V., Oujifard, A., Mousavi, S.M., Gisbert, E. & Mozanzadeh, M.T. (2018). Dietary nucleotide mixture effects on reproductive and performance, ovary fatty acid profile and biochemical parameters of female Pacific shrimp Litopenaeus vannamei. Aquaculture Nutrition, 24, 515– 523.
Artiss, J.D. & Zak, B. (1997). Measurement of cholesterol concentration. Handbook of Lipoprotein Testing. AACC Press, Washington: 99- 114.
Banaee, M., Sureda, A., Mirvaghefi, A.R. & Rafei, G.R. (2011). Effects of long-term silymarin oral supplementation on the blood biochemical profile of rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 37, 885–896.
Basiglio, C.L., Sa´nchez Pozzi, E.J., Mottino, A.D. & Roma, M.G. (2009). Differential effects of silymarin and its active component silibinin on plasma membrane stability and hepatocellular lysis. Chemico-Biological Interact, 179, 297–303.
Bauer, P.J. (1981). Affinity and stoichiometry of calcium binding by arsenazo III. Analytical Biochemistry, 110(1), 61- 72.
Bendich, A. (1989). Cartenoids and the Immune response. Nutrition, 119(1), 112-115.
Borsari, M., Gabbi, C., Ghelfi, F., Grandi, R., Saladini, M., Severi, S. & Borella, F. (2001). Silybin, a new iron-chelating agent. Journal of Inorganic Biochemistry, 85, 123–129.
Cárdenas, W. & Dankert, J.R. (1997). Phenoloxidase specific activity in the red swamp crayfish Procambarus clarkia. Fish & Shellfish Immunology, 7, 283–295.
Chen, J.C., Lin, M.N., Ting, Y.Y. & Lin, J.N. (1995). Survival, haemolymph osmolality and tissue water of Penaeus chinensis juveniles acclimated to different salinity and temperature levels. Comparative Biochemistry and Physiology, 110 A, 253-258.
Chew, B.P. & Park, J.S. (2004). Carotenoid action on the immune response. Journal of Nutrition, 134, 257S–261S.
Chuchird, N., Rorkwiree, Ph. & Rairat, T. (2015). Effect of dietary formic acid and astaxanthin on the survival and growth of Pacific white shrimp (Litopenaeus vannamei) and their resistance to Vibrio parahaemolyticus. Springer Plus, 4:440, 1-12.
da Silva, B.C., do Nascimento Vieira, F., Mouriño, J.L.P., Ferreira, G.S. & Seiffert, W.Q. (2013). Salts of organic acids selection by multiple characteristics for marine shrimp nutrition, Aquaculture, 384–387, 104-110.
Ebrahimi, A. & Beiraghdar, A. (2006). Nutrition and fish requirements in aquaculture (with emphasis on cultural species in Iran). Isfahan University of Technology, Jihad Daneshgahi Publications. 1st edition, 304 pages (in Persian Language).
El-Kamary, S.S., Shardell, M.D., Abdel-Hamid, M., Ismail, S., El-Ateek, M., Metwally, M., Mikhail, N., Hashem, M., Mousa, A., Aboul-Fotouh, A., El-Kassas, M., Esmat, G. & Stricklanda, T. (2009). A randomized controlled trial to assess the safety and efficacy of silymarin on symptoms, signs and biomarkers of acute hepatitis. Phytomedicine, 16, 391–400.
Ellis, A.E. (1990). Lysozyme Assays. Techniques in fish immunology, 101-103.
Foster, L.B. & Hocholzer, J.M. (1971). A single reagent manual method for directly determining urea nitrogen in serum. Clinical Chemistry, 17, 921-925.
Fouladi, P., Ebrahimnezhad, Y., Shahryar, H.A., Maheri, S.N. & Ahmadzadeh, A.R. (2014). Effects of organic acids supplement on performance and gut parameters in male Japanese quail (Coturnix coturnix). Journal Biological International, 6(2), 102-109.
Gislason, G., Olsenm R.E. & Ringø, E. (1994). Lack of growth-stimulating effect of lactate on Atlantic salmon, Salmo salar L. Aquaculture and Fisheries Management, 25, 861- 862.
He, W., Rahimnejad, S., Wang, L., Song, K., Lu, K. & Zhang, Ch. (2017). Effects of organic acids and essential oils blend on growth, gut microbiota, immune response and disease resistance of Pacific white shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus, Fish & Shellfish Immunology, 70, 164-173.
Jiravanichpaisal, P., Lee, B.L. & Söderhäll, K. (2006). Cellmediated immunity in arthropods. hematopoiesis, coagulation, melanization and opsonization. Immunobiology, 211, 213-36.
Johansson, M.W., Keyser, P., Sritunyalucksana, K. & Söderhäll, K. (2000). Crustacean haemocytes and haematopoiesis. Aquaculture, 191 (1–3), 45–52.
Khan, M.U., Qurashi, N.A., Khan, M.S., Jabeen, F., Umar, A., Yaqoob, J. & Wajid. M. (2016). Generation of reactive oxygen species and their impact on the health-related parameters: A critical review. International Journal of Biosciences, 9(1), 303-323.
Le Moullac, G., Le Groumelle, M., Ansquer, D., Froissard, S. & Levy, P. (1997). Haematological and phenloxidase activity change in the shrimp Penaeus stylirostris in relation with the moult cycle: protection against vibriosis. Fish & Shellfish Immunology, 7, 227-234.
Li, J.T., Lee, P.P., Chen, O.C. & Chen, W. (2005). Dopamine depresses the immune ability and increases susceptibility to Lactococcus garvieae in the freshwater giant prawn, Macrobrachium  rosenbergii. Fish & Shellfish Immunology, 19, 269-280.     
Maktabi, P., Mohammadi Azarm, H., Peighan, R., Mousavi, S.M. & Zarei, M. (2019). Effect of Formic Acid, Potassium D-Format Salt and Formic Acid Nanochitosan Solution on Survival Rate, Growth Parameters and Carcass Composition in Common Carp (Cyprinus carpio). Journal of Wetland Ecological Research, Islamic Azad University, 10 (38), 41-54. (in Persian Language).
Menanteau-Ledouble, S., Krauss, I., Goncalves, R.A., Weber, B., Santos, G.A. & El-Matbouli, M. (2017). Antimicrobial effect of the Biotronic® Top3 supplement and efficacy in protecting rainbow trout (Oncorhynchus mykiss) from infection by Aeromonas salmonicida subsp. Salmonicida. Research in Veterinary Science, 114, 95–100.
Mente, E., Karalazos, V., Karapanagiotidis, I. & Pita, C. (2011). Nutrition in organic aquaculture: an inquiry and a discourse. Aquaculture Nutrition, 17, 798-817.
Ng, W.K., Koh, Ch.B., Teoh, Ch.Y. & Romano, N. (2015). Farm-raised tiger shrimp, Penaeus monodon, fed commercial feeds with added organic acids showed enhanced nutrient utilization, immune response and resistance to Vibrio harveyi challenge, Aquaculture, 449, 69-77.
Ozbay, G. & Riley, J.G. (2002). An analysis of refractometry as a method of determining blood total protein concentration in the American lobster Homarus americanus (Milne Edwards). Aquaculture Research, 33, 557-562.
Punitha, S.M.J., Babu, M.M., Sivaram, V., Shankar, V.S., Dhas, S.A., Mahesh, T.C., Immanual, G. & Citarasu, T. (2008). Immunostimulating influence of herbal biomedicines on nonspecific immunity in Grouper Epinephelus tauvina juvenile against Vibrio harveyi infection. Aquaculture International Journal, 16, 511-523.
Rahman, M.M., Normawaty, M.N., Shahbudin, S. & Kamaruzzaman, Y. (2015). Effects of human activity and the distance from shoreline. Desalin Water treat. Coastal water quality of Tioman Island. Doi: 10.108./ 19443994. 2. 15. 100682.
Ramadan, L.A., Roushdy, H.M., Abu-Senna, G.M. & Amin, N.E. (2002). Radio-protective effect of silymarin against radiation induced hepatotoxicity. Pharmacological Research, 45(6), 447–454.
Rifai, N., Bachorik, P.S. & Albers, J.J. (1999). Lipids, lipoproteins, and apolipoproteins.In: Burtis, C.A., Ashwood, E.R. (Eds.), Tietz Textbook of Clinical Chemistry. WBSaunders, Philadelphia, Pennsylvania, pp. 809–861.
Romano, N., Koh, Ch.B. & Ng, W.K. (2015). Dietary microencapsulated organic acids blend enhances growth, phosphorus utilization, immune response, hepatopancreatic integrity and resistance against Vibrio harveyi in white shrimp, Litopenaeus vannamei, Aquaculture, 435, 228-236.
Sacks, D.B. (2011). A1C Versus glucose testing: a comparison, Diabetes Care, 34, 518-523.
Saei, M.M., Beiranvand, K., Taee, H.M. & Nekoubin, H. (2016). Effects of Different Levels of BioAcid Ultra on Growth Performance, Survival, Hematological and Biochemical Parameters of Fingerlings Rainbow Trout (Oncorhynchus mykiss). Journal of Aquaculture Research and Development, 7(455), 1-5.
Sakai, M. (1999). Current research status of fish immunostimulants. Aquaculture, 172(1–2), 63-92.
Samadi, L., Zanguee, N., Mousavi, S.M. & Zakeri, M. (2016). Effect of dietary Garlic extract on growth, feeding parameters, hematological indices and body composition of Litopenaeus vannamei. Journal of the Persian Gulf (Marine Science), 7(24), 29-41.
Shah, S.Z.H., Afzal, M., Khan, S.Y., Hussain, S.M. & Habib, R.Z. (2015). Prospects of using citric acid as fish feed supplement. International Journal of Agriculture and Biology, 17, 1−8.
Shahsavani, D., Kazerani, H.R., Kaveh, S. & Gholipour-Kanani, H. (2010). Determination of some normal serum parameters in starry sturgeon (Acipenser stellatus Pallas, 1771) during spring season. Comparative Clinical Pathology, 19, 57–61.
Sheikh, A., Tufail, B., Gulam, A.B., Masood, S.M. & Manzoor, R. (2010). Effect of dietary supplementation of organic acids on performance, intestinal, histomorphology and serum biochemistry of broiler chickens. Veterinary Medicine International, 479485-479487.
Sobolova, L., Skottova, N., Vecera, R. & Urbanek, K. (2006). Effect of silymarin and its polyphenolic fraction on cholesterol absorption in rats. Pharmacological Research, 53, 104–112.
Söderhäll, K. & Hall, L. (1984). Lipopolysaccharide-induced activation of the prophenoloxidase activating system in crayfish haemocytelysate. Biochimica et Biophysica Acta, 797, 99-104.
Song, L., Yu, I., Lien, W. & Huang, C. (2003). Haemolymph parameters of Litopenaeus vannamei infected with Taura syndrome virus. Fish & Shellfish Immunology, 14, 317-331.
Stickel, F., Patsenker, E. & Schuppan, D.  (2005). Herbal hepatotoxicity. Journal of Hepatology, 43, 901–910.
 Su, X., Li, X., Leng, X., Tan, C., Liu, B., Chai, X. & Guo, T. (2014). The improvement of growth, digestive enzyme activity and disease resistance of white shrimp by the dietary citric acid. Aquaculture International, 22, 1823–1835.
Wang. F.I. & Chen. J.C. (2006). The immune response of tiger shrimp Penaeus monodon and its susceptibility to Photobacterium damselae subsp. Damselae under temperature stress. Aqaculture, 258, 34-41.
Zahizadeh, A., Zakeri, M., Mousavi, S.M., Kochanian, P. & Souri, M. (2020). Effect of different levels of dietary Biotronic Top3 on growth performance, feed utilization and body biochemical composition of the white leg shrimp (Litopenaeus vannamei). Journal of Fisheries, 73(4), 515-527.
Zakaria, H. & Sodagar, M. (2016). Aquatic plants and their role in the aquarium. Ornamental Aquatic Extension Scientific Journal. 3(2), 25-30. (in Persian Language).

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