Study of phylogenetic background and antibiotic resistance of Escherichia coli isolates obtained from healthy household dogs and their owners in Kerman city

Document Type : Research Paper

Authors

1 Researcher, Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences and PhD Graduated in Bacteriology, Department of Pathobiology, Faulty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

2 Professor, Molecular Microbiology Research Group, Shahid Bahonar University of Kerman, Kerman, Iran

3 Assistant Professor, Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran

4 Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

5 Professor, HIV/STI Surveillance Research Center, and WHO Collaborating Center for HIV Surveillance, Institute for Futures Studies in Health and Professor, Department of Biostatistics and Epidemiology, Faculty of Public Health, Kerman

6 Assistant Professor, Department of Pathobiology, Faulty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

Abstract

    The dogs' relationship with their owners has made them one of the most popular pets. They can be a reservoir of many microbial pathogens, so they are important for public health. This study was performed to determine the phylogenetic backgrounds and antimicrobial resistance of E. coli isolates from healthy household dogs and their owners in Kerman province, Iran. Samples were taken regardless of antibiotic usage in the dogs. Based on the history of the animals, 90% of them had not used antibiotics during the few months before the study. 168 E. coli strains belonging to feces of healthy household dogs (n=49), their owners (n=49) and the people without a pet (n=70) were studied; phylogenetic sequences including chuA, yjaA and TspE4.C2 were screened by conventional polymerase chain reaction (PCR). The isolates were investigated phenotypically for antimicrobial resistance against 11 commonly used antibiotics in dogs which were erythromycin, streptomycin, enrofloxacin, oxytetracycline, cefotaxime, ampicillin, trimethoprim-sulfamethoxazole, amoxicillin-clavulanic acid, ceftazidime, ceftriaxone and kanamycin. E. coli isolates were classified into A, D, B1 and B2 phylogroups with the prevalence of 55.9%, 30.3%, 7.1% and 5.3%, respectively. Considerably, antimicrobial resistance to erythromycin and oxytetracycline were predominant while the lowest frequency of antibiotic resistance was detected against ceftriaxone, ceftazidime, amoxicillin-clavulanic acid and kanamycin. This study was performed on apparently healthy dogs so it could determine their carrier role for antibiotic-resistant E. coli strains. This research revealed that healthy household dogs should be considered as the significant reservoir of resistant E. coli isolates especially to erythromycin, oxytetracycline, streptomycin, enrofloxacin, cefotaxime and ampicillin which these resistant strains were mostly belonging to A and D phylogenetic groups.

Keywords

References

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