Histopathological, molecular and serological study of Johnes disease in buffaloes of Ahvaz

Document Type : Research Paper

Authors

1 PhD Student of Veterinary Pathology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

5 Associate Professor, Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

    Johne’s Disease (paratuberculosis) is granulomatous, progressive enteritis, which is untreatable, especially in domestic and wild ruminants caused by mycobacterium avium subsp. paratuberculosis. There is no accurate statistical information on the prevalence of Johne’s disease in buffaloes of different regions of the country. This study was performed to detect mycobacterium avium subsp. paratuberculosis with different diagnostic methods: ELISA, PCR, Histopathology and their comparison. For this purpose, from December 2016 to October 2017, Among 97 slaughtered buffaloes, above two years of age with poor physical conditions were collected from blood samples, associated ileocecal lymph nodes, ileocecal valve and liver tissue were transferred to the laboratory. The serum level of infection (ELISA) was 4.1%, The molecular (PCR) test showed an infection rate of 32% in the intestin , 31% in the associated lymph node and 24.7% in the liver, Histopathology examination by Ziehl-Neelsen's specific staining, The prevalence of infections in the intestines are 7.2%, associated lymph nodes are 8.2% and 3.1% in the liver. The granulomatous lesions of the intestine were stained by hematoxylin-eosin (H&E) method in 11 buffaloes, only in 9 buffaloes their molecular test results were positive. Other histopathology findings of eosinophilic enteritis, Lymphocytic enteritis, granulomatous enteritis, mucu purulent enteritis were 37.9%, 29.3%, 19%, 13.8%, respectively. In the associated mesenteric lymph nodes, granulomatous lymphadenitis was 68.6%, purulent lymphadenitis were 25.7%, and in liver chronic hepatitis were 32.6%, chronic parasitic hepatitis 11.66%, perihepatitis 23.9%, cirrhosis 6.5%, and leiomyoma 25% have been observed. Due to the lack of observation of acid-fast bacilli in the present study, acid-fast granular debris (Paucibacillary) form of suspected Johneʼs disease can be found. At the same time, the increase of Ahvaz buffaloes contamination requires more serious attention to scientific and administrative references than the previous study.

Keywords

References

Antognoli, M.C.; Garry, F.B.; Hirst, H.L.; Lombard, J.E. and Salman, M.D. (2008). Characterizaton of Mycobacterium avium subsp. Paratuberculosis disseminated infection in dairy cattle and its association with antemortem test results. Veterinary microbiology, 127(3): 300-308.
Anwar Khan, F.; Iqbal Chaudhry, Zafar.; IjZ Ali, Muhammd.; Khan, Shahid.; Mumtaz, Naima and Ahmad, Ijaz. (2010). Detection of Mycobacterium avium subsp. Paratuberculosis in tissue samples of cattle and buffaloes. Tropical Animal Health and Production journal, 42(4): 633-638.
Baker, D.C.; Barker, I.K.; Brown, C.C.; Caswell, J.L. and Wittiams, K.J. (2007). Jubb, Kennedy and Palmer's Pathology of Domestic Animals, Vol. 2, 5th ed. Guelph, Ontario Canada, Pp:192-196.
Chaturvedi, S.; Singh, S.; Srivastava, A.; Gangwar, N.; Kumar, N.; Rawat, K.; et al. (2017). Comparative evaluation of FAT, IS900 PCR and microscopy vis a vis histopathology for the detection of Mycobacterium avium subsp. Paratuberculosis infection in tissues of goats naturally died in herds endemic for Johne's disease. Indian Journal of animal science, 87(6): 658-693. 
 Desio, G.; Nizza, S.; Montagnaro, S.; Sasso, S.; De Matrino, L. and Lovane, V. (2013). Estimated prevalence of Johne’s disease in herds of water buffaloes (Bubalus bubalis) in province of Caserta. Italian Journal of Animal Science, 12(8): 48-52.
 Gonda, M.G.; Chang, Y.M.; Shook, G.F.; Collins, M.T. and Kirkpatrick, B.W. (2007). Effect of Mycobacterium paratuberculosis infection on production, reproduction, and health traits in US Holsteins. Preventive Veterinary Medicine, 80(2):103-119.
 Gumussoy, K.S.; Abay, S.; Ica, T.; Abay, S.; Aydin, F. and Hizlisoy, H. (2015). Serological and molecular diagnosis of paratuberculosis in dairy cattle. Turkish Journal of Veterinary and Animal Sciences, 39:147-153.
 Gwozdz, J.M.; Thompson, K.A.; Reichi, M.P.; Murray, A.; Manktelow, B.W.; West, D.M. et al. (1997). Detection of Mycobacterium avium subsp. Paratuberculosis in ovine tissue and blood by PCR. Veterinary Microbiology 5th ed. Pp: 233-244.
 Hailat, N.; Hemida, H.; Hananeh, W.; Stabel, J.; Rezig, F.; Jaradat, S. et al. (2012). Investigation on the occurrence and pathology of paratuberculosis (Johne's disease) in apparently cattle in Jordan. Comprative Clinical Pathology, 21(5): 879-888.
 Hajikolaei, M.; Ghorbanpoor, M. and Amirsoleymani, M. (2008). Study on Mycobacterium avium subsp. Paratuberculosis in buffaloes at Ahwaz abattoir. Iranian Journal of Vet Clinical Sciences Shahrekord University, 2(1): 55-60. (in persian)
Heidarnejhad, O.; Safi, Sh.; Mosavari, N. and Keshavarz, R. (2017). Sero-prevalence of subclinical paratuberculosis (Johne's disease) in dairy farms of Tehran-Iran using absorbed ELISA assay. Journal of Comparative Pathobiology, 14(3): 2239-2246. (in persian)
 Karimi, H.; Namjoo, A.; Momtaz, H. and Namdari, M. (2011). Identification of Mycobacterium avium subsp. Paratuberculosis in ileum tissue samples in buffaloes at Shahrekord abattoir using ELISA and PCR methods. Journal of Comparative Pathobiology Azad University, 8(4): 697-704. (in persian).
 Kheirandish, R.; Sami, M.; Khalili, M.; Shafaei, K. and Azizi, S. (2016). Diagnosis of paratuberculosis in fresh & paraffin embedded samples by histopathology, PCR and immunohistochemistry techniques. Bulgarian Journal of Veterinary Medicine. Bulgarian Journal of Veterinary Medicine, 20(4): 50-60.
Kulsrestha, R.C.; Singh, J. and Chandirmani, N.K. (1980). A survey on the prevalence of tuberculosis and Johne’s disease in cattle and buffalo in Haryana state. Haryana Veterinarian, 19(2): 139-141.
Kurade, N.P.; Tripathi, B.N.; Rajukumar, K. and Parihar, N.S. (2004). Sequential development of histologic lesions and their relationship with bacterial isolation, fecal shedding, and immune responses during progressive stages of experimental infection of lambs with Mycobacterium avium subsp. Paratuberculosis. Veterinary Pathology, 41(4):378-387.
 Momotani, E.; Whipple, D.L.; Thiermann, A.B. and Cheville, N.F. (1988).  Role of M cells and macrophages in the entrance of Mycobacterium paratuberculosis into domes of ilealPeyer's patches in calves.Veterinary Pathology, 25(2): 131-137.
 Mutharia, L.M.; KlaSSEN, M.D.; Fairles, J.; Babut, S. and Gill, C.O. (2010). Mycobacterium avium subsp. Paratuberculosis in muscle, lymphatic and organ tissues from cows with advanced Johne’s disease. International Journal of Food Microbiology, 136(3):340-344.
OIE Terrestrial Manual (2014): Chapter 2.1.11. Paratuberculosis (John’s disease).
 Rajabian Gharib, F. and Khosravi Pour, B. (2016). Socio-economic aspects of buffalo breeding.  Journal of Science and Technology Veterinary Histobiology, 4(1): 4-7. (in persian)
 Rehman, A.; Javed, M.; Rizvi, F. and Khan, M. (2017). Prevalence and Pathology of Paratuberculosis in cattle and Buffaloes at Faisalabad Abattoir. Pakistan Journal of Agriculture and Science, 54(1): 189-194.
Rossiter, C.A. and Henning, W.R. (2001). Isolation of  Mycobacterium avium subsp. Paratuberculosis from thin market cows at slaughter. Journal of Animal Science, 79(4): 113-114.
 Sikandar, A.; Cheema1, A.H.; Younus, M.; Aslam1, A.; Zaman, M.A. and Rehman, T. (2012). Histopathological and Serological Studies on Paratuberculosis in Cattle and Buffaloes. Pakistan Veterinary Journal, 32(4): 547-551.
 Singh, S.V.; Singh, R.; Sharma, S.; Shukla, N.; Misra, S.; Singh, P.K. et al. (2008): Sero-prevalence of Bovine Johne’s disease in buffaloes and cattle population of North India using indigenous ELISA kit based on native Mycobacterium avium subsp. Paratuberculosis “Bison type” of  goat origin. Comparative Immunology, Microbiology and Infectious Diseases, 31(5): 419-433.
 Sivakumar, P.; Tripathi, B.N.; Singh, N.; EzhilPraveena, P. and Saravanan, D. (2007). Seroprevalence of paratuberculosis in Indian buffaloes indian. Jurnal of  Veterinary Pathology, 31(1): 62-63.
 Sivakumar, P.; Tripathi, B.N; Singh, N. and Sharma, A.K. (2006). Pathology of naturally occurring paratuberculosis in water buffaloes (Bubalus bubalis). Veterinarian Pathology. 43(4): 455-462.
 Smith, S.L.; Wilson, P.R.; Collett, M.G.; Heuer, C.; West, D.M.; Stevenson, M. and Chamber, J.P. (2014). Liver Biopsy Histopathology for Diagnosis of Johne’s Disease in Sheep. Veterinary Pathology, 51(5): 915-918.
 Sohal, J.S.; Singh, S.V.; Subhodh, S.; Singh, A.V.; Singh, P.K. and Sheoran, N. (2007). Mycobacterium avium subspecies paratuberculosis diagnosis and strain typing present status and future developmants. Indian Journal Experimental Biology. 45(10): 843-852.
 Sweeney, R.W.; Whitlock, R.H.; Buckley, C.L. and Spencer, P.A. (1995). Evaluation of a commercial enzyme-linked immune sorbent assay for the diagnosis of paratuberculosis in dairy cattle, Journal of Veterinary Diagnostic Investigation, 7(2): 488-495.
 Tiwari, A.; VanLeeuwen, J.A.; McKenna, S.L.B.; Keefe, G.P. and Barkema, H.W. (2006). Johne’s disease in Canada. Part 1.Clinical symptoms, pathophysiology, diagnosis and prevalence in dairy herds. The Canadian Veterinary Journal. 47(9): 874-882.
 Yegani, M. (1998). Johne's diseases.1th ed, Iran Veterinary Organization, Pp: 68-76, 78-80. (in persian)

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