Iranian Veterinary Journal

Iranian Veterinary Journal

Determination of phylogenetic groups, phenotypic identification of broad-spectrum beta-lactamases, and determination of antibiotic sensitivity profiles in Escherichia coli isolates in diarrheal and non-diarrheic dogs

Document Type : Research Paper

Authors
Feryal Toiemehpoor 1
Bahman Mosallanejad 2
Dariush Gharibi 3
Mohammad Khosravi 4
Mahdi Pourmahdi Borujeni 5
1 PhD Graduated in Small Animal Internal Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4 Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
5 Professor, Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
10.22055/ivj.2023.398000.2584
Abstract
    Escherichia coli is an important member of the Enterobacteriaceae family, that can cause disease(s) in humans and a wide range of animals, including dogs and cats. The aim of the present study was to determine the phylogenetic groups and phenotypic identification of extended-spectrum beta-lactamases (ESBL) in Escherichia coli isolates obtained from diarrheal and non-diarrheic dogs and to detect the antibiotic sensitivity profile of positive ESBL isolates. The cultivation of stool samples in selected environments and confirmation of the resulting isolates were obtained by performing additional biochemical tests. Determination of the phylogenetic groups was done in the isolates by the Claremont method using primers ChuA, YjaA, TspE4.c2, and arpA; Moreover, phenotypic identification of ESBL was approved by the combined disc method and the CLSI protocol. From one hundred and fifty stool samples, 182 Escherichia coli isolates were obtained eventually. From this number, 100 isolates were selected to determine the phylogenetic groups and phenotypic identification of ESBL-producing Escherichia coli. Phylogenetic analyses showed that Escherichia coli isolates from dogs belonged to phylogeny groups A, B1, B2, D and F, with prevalence rates of 39%, 42%, 4%, 9% and 6%, respectively. Also, the frequency of phylogenetic groups in diarrheal dogs was (42.3%) in group A, (38.5%) in B1, (9.6%) in D, (5.8%) in F, and (3.9%) in B2. In addition, in healthy dogs, the phylogeny groups were in (43.07%) in group B1, (39.26%) in A, (7.7%) in D, (6.2%) in F and (3.96%) in B2. Out of 100 Escherichia coli isolates, 31 isolates (31%) were phenotypically beta-lactamase ESBLs producers. Twelve isolates (38.7%) were related to diarrhea samples and 19 isolates (61.3%) were related to healthy dogs. The high prevalence of Escherichia coli isolates producing ESBL enzymes in Ahvaz district increases the risk of spread and transmission of ESBL-producing strains in the pet population and their transmission to the human population.
Keywords
Escherichia coli
Phylogenetic group
Beta-lactamase
Antibiotic resistance
Dog
Diarrhea

Subjects

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