This study was conducted to investigate the synergy effect of sesame oil (SEO) and olive leaf extract (OLE) as sources of natural antioxidants of diet on the morphology of the jejunum and blood parameters of broilers from 7 to 28 days of age. One hundred fifty Ross male broilers were randomly distributed to 30 experimental units and 6 dietary treatments (5 replicates with 5 birds in each). The completely randomized design with factorial arrangement 3×2 with 3 levels of OLE (0, 120 and 240 mg/kg of diet) and 2 types of vegetable oil [crude corn oil (CRO) and SEO] were used. The experimental diets included: a corn soybean meal based diet with added CRO and without OLE supplementation, a basal diet with SEO and without OLE supplementation, a basal diet with added CRO and 120 mg/kg OLE supplementation, a basal diet with added SEO and 120 mg/kg OLE supplementation, a basal diet with added CRO and 240 mg/kg OLE supplementation and a basal diet with added SEO and 240 mg/kg OLE supplementation. Chicks fed with diet containing CRO and without OLE had the lowest lenght jejunum villi (1085 µm) (P<0.05). Replacement of SEO with CRO in the diet resulted in a significant increase in villus width and a significant decrease in serum triglyceride levels of broilers (P<0.05). The inclusion of OLE in diet significantly decreased the serum glucose and malondialdehyde (P<0.05). It demonstrated that concurrent use of OLE and SEO in broiler diets from 7 to 28 days of age, could be positively influence on blood parameters and gastrointestinal health due to their strong antioxidant compounds.