Determination of FecB and FecGH mutations in Lory goats of Lorestan province

Document Type : Research Paper

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Abstract

    Determination of related genes to prolificacy is very important for animal improvement. FecB, FecG and FecX are the important genes on the fecundity. The aim of this study was the determination of FecB and FecGH mutations in Lory goats of Lorestan Province. For this study 50 blood samples were collected from Lory goats. DNA was extracted by modified salting out method. Site of the mutations were amplified using specific primers and PCR products (190 bp and 139 bp bands) were determined by agarose gel electrophoresis, and then the PCR products were digested with AvaII and DdeI enzymes. Results show no mutations of FecB and FecGHin the Lory goats. The genotype of all goats were wild type. In conclusion, the mutations of FecB and FecGH are not cause of prolificacy in Lory goats. So, further study is required to determine the fecundity genes and genotyping of Lory goats.

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