Cloning and expression of outer membrane protein 31 (Omp31) Brucella melitensis Rev1

Document Type : Research Paper

Authors

1 PhD Graduated of Genetics and Animal breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2 Professor, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

3 Assistant Professor, Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Brucellosis is a well-known infection among domestic animals which caused by Brucella bacterium. Omp31 is an outer membrane protein that play important roles in simulate CD4+ T and CD8+ T cells. In current study molecular, cloning and also phylogenic analysis of Omp31 gene as a candidate for designing subunit vaccine against brucella was investigated. Amplifying performed using specific primers. Cloning of this gene performed using pMB57R/T vector in TOP10F`strain of E.coli as host. Also, pET32a vector used for expression. Omp31 gene with 723 bp amplified successfully. After that clone using TA cloning approach within cloning vector. Expression of this gene has done in pET32a vector by inducing IPTG. Results confirmed with sequencing and SDS PAGE showed 48 KD protein band correctly. According this results we can propose this gene as a candidate for designing subunit vaccine against brucella in future study.According this results we can propose this gene as a candidate for designing subunit vaccine against brucella in future study.

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