Histologغl and micrometقغ of Gut Associated Lymphatic Tissue (GALT) in mature and immture Silver carp fish

Document Type : Research Paper

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Abstract

    Gut Associated Lymphoid tissue (GALT) is a primary lymphoid tissue in mammals and it is a first defense barrier for pathogen factors. There are many reports about existence of inter and intra species differences in fish GALT structure. In this study 10 Silver fish (5 mature and 5 immature) were selected and 0.5 cm samples were taken from intestine bulb to terminal part of main intestine (colon). The 5-6 µm sections were made using paraffin embedding method. The sections were stained by H&E and PAS. The results showed that the GALT are located in 3 regions and in 2 types. The first, distributed in epithelial tissue, the second location was in lamina propria as columnar or aggregated shapes, and the third location was in submucosa. The lymphoid tissue is consisted mainly from lymphocytes, lymphoblasts, a few plasma cells and macrophages. There is a significant difference in intraepithelial lymphocytes number in mature and immature fish (P<0.05). The maximum lymphocytes were seen in intestinal bulb and main intestine of both mature and immature fish. The results showed that there are significant differences in location, shape and distribution of lymphoid tissue in silver carp fish.

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