Effects of sainfoin tannin treated by water or urea on microbial population, gas production parameters, digestibility and in vitro fermentation

Document Type : Research Paper

Authors

1 PhD Graduated of Animal Nutrition, Faculty of Animal and Food Science, Ramin Agricultural and Natural Resources University of Khuzestan, Iran

2 Associate Professor, Department of Animal Science, Faculty of Animal and Food Science, Ramin Agricultural and Natural Resources University of Khuzestan, Iran

3 Assistant Professor, Department of Animal Nutrition, Research Institute of Animal Sciences, Karaj, Iran

Abstract

This experiment with aim to determine the effect of the tannins in dry land and irrigated sainfoin forage treated by water or urea on gas production (GP) parameters, in vitro fermentation characteristics and microbial population was carried out. Treatments were consist of untreated irrigated sainfoin (UNIS), untreated dry land sainfoin (UNDS), irrigated sainfoin by water (ISTW),dryland sainfoin treated by water (DSTW), irrigated sainfoin treated by urea (ISTU) and dry land sainfoin treated by urea (DSTU). Data of GP method were analyzed by Repeated Measurement and data other analyzed with CRD design. The results showed that treated by water or urea was due to reduce dry matter (DM). The most crude protein (CP) was relation to treated by urea (14.25%). The effect of treated by water or urea had the most decreasing of total phenolic compounds, total and condensed tannin in irrigated sainfoin (P<0.05).The results showed ISTW and ISTU treatments were highly on potential of GP and gas production rate (GPR).Increasing of GP in ISTW and ISTU treatments indicated detanning effect of treated by water or urea. The most truly degraded substrate )TDS) was related to ISTW treatment and the least UNDS (296 and 105mg, respectively). Partitioning factor in ISTW treatment (6.01) was significantly difference other treatments (P<0.05). Sainfoin tannin was due to decrease GP in total process of ruminal fermentation.The least N-NH3 concentrations was related to UNDS and the most were ISTU and DSTU treatments (P<0.05). The effect of treatments on the total number of rumen bacteria, cellulolytic bacteria and protozoa were significant (P<0.05). Overall, treated sainfoin by urea or water was due to no change of total number of rumen bacteria, cellulolytic bacteria and protozoa and increasing of the microbial biomass efficiency and synthesis. Generally, treated by water was more successful and cheaper method than the use of urea.

Keywords


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