Typing of Clostridium perfringens isolates from sheep in Ahvaz

Document Type : Research Paper

Authors

1 Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Professor, Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 DVM Graduated from faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Clostridium perfringens is an important pathogen which causes numerous different ‎diseases in animals. This bacterium is classified into five different types; A, B, C, ‎D and E. Clostridium perfringens type D cause entrotoxemia in animals.‎
The aim of this study was to identify different types of Clostridium perfringens in ‎sheep in Ahvaz. For this purpose 369 fecal samples were randomly collected from ‎the sheep from different area. After processing and culturing samples in blood ‎agar containing neomycin, Clostridium perfringens suspected isolates were ‎identified morphologically and biochemically. According to biochemical tests, ‎from 369 feces samples, 56 isolates obtained and identified as Clostridium ‎perfringens. For molecular confirmation of the isolate, the DNA of the isolates ‎was extracted and the isolates were verified as Clostridium perfringens by PCR ‎using specific primers for alfa toxin. The results showed that 54 isolated from 56 ‎isolated confirmed as Clostridium perfringens. Typing of these isolates was done ‎by PCR using specific primers for 5 different exotoxins of this bacterium. The ‎results showed that 10 isolates (18/51%) were type A and 4 (7/4%), 24 (44/44%) ‎and 16 isolates (29/62%) were type B, type C and type D respectively. Thus the ‎most prevalent types of Clostridium perfringens were type C and D. In the present ‎study, all isolates were negative for iota toxin and enterotoxin gene. ‎

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References

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