Compare the total immunoglobulin assays using turbidity and precipitation test in common carp exposed to synthesized silver nanoparticles using sargassum seaweed

Document Type : Research Paper

Authors

1 Assitance Professor, Department of Fisheries, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran

2 Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Associate Professor of Basic Sciences department, Faculty of Veterinary, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Immunoglobulins are serum proteins that produced by the plasma and humoral immune responses are important components commonly by serological methods for the measurement. In this study, at first the synthesis of silver nanoparticles was performed using biological method with sargassum seaweed, then the impact of this nanoparticle on the total immunoglobulin of common carp using two different methods of turbidity and precipitation test The impact of nanoparticles on the total immunoglobulin using two methods: turbidity and sediment exposed to three concentration (0.11, 1.13, 5.67 mg/L AgNP) of this silver nanoparticles were studied for 14 days.. using were compared. Results obtained from UV-Vis spectroscopy and TEM confirmed biosynthesis of silver nanoparticles using this algae. The synthesized silver nanoparticles were predominately spherical shape and 32/54 nm size and maximum absorption peak were detected in the range of 406 nm. The results of the measurement of serum immunoglobulin using turbidity and precipitation tests indicating no significant differences in the treatments with control (p>0.05). As well as between the amount of serum immunoglobulin in each treatment sample at different days showed no statistically significant difference (p>0.05). In conclusion our results indicate that silver nanoparticles synthesized using Sargassum angustifolium, had no effect on total serum immunoglobulin levels measured by both methods inhibition or stimulation.

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References

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