Effects of Cholesterol loaded cyclodextrin on vitrification of the cat epididymal sperm

Document Type : Research Paper

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Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

    Cholesterol loaded cyclodextrin (CLC) efficiently protected sperm of some species against cryodamage. On the other hand, vitrification of sperm especially in human opened a new horizon in the field of sperm freezing. The aim of the present study was to evaluate the effect of CLC on vitrification of cat sperm. The tom epididymal sperm (n=22 pairs) was extracted from epididymides and subjected to vitrification in TRIS based solution that was incorporated with or without CLC. The warmed sperm was analyzed for motility, viability and morphology. Vitrification significantly (P<0.0001) reduced the sperm motility (%) in CLC-plus (3.6±2.28) and CLC-minus (1.1±2.02) compared to the fresh samples (28.3±1.82). Sperm viability was also significantly decreased the following vitrification either with CLC (80.6±2.44) or without CLC (78.2±2.15) compared to the fresh samples (89.9±1.94; P=0.0011). In spite of the significant impact of vitrification on cat epididymal sperm, it seems that there is an indigenous tolerance to the vitrification procedure and CLC did not have more beneficial effect on it.

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