Document Type : Research Paper
Authors
1
Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2
Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz-Iran.
3
Associate professor in veterinary surgery, Department of Food Hygiene, shahid chamran university of ahvaz, ahvaz, Iran.
4
DVSc Graduated of Veterinary Surgery, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract
Thousands of years before the plants have been used as a wound healing material. Typha angustifolia is native plant in wetland environments such as Khuzestan, Iran. It is available in abundance and extraction. The aim of this study was to evaluate the effect of Typha angustifolia pollen extract on experimental full thickness wound healing in mice. Thirty six mice were equally divided into 3 groups: the control group of saline, control group of eucerin and the treatment group (eucerin containing pollen extract), respectively. After anesthesia, a piece of 0.5 * 0.5 cm in size of skin was removed from the back of the neck and between the shoulders. The surgical site was treated with topical saline sterile. This treatment procedure was done for all 12 mice in each group and after each group received its specific treatment. Each of the groups was also divided into 3 subgroups (group 7, 14 and 21 days). At the end of each period in each group and after euthanasia of each mouse, wounded skin removed with 0.5 cm of surrounding skin and sent to histopathology laboratory. At the end of the study, mononuclear cells, polymorphonuclear, fibroblasts and angiogenesis was lower than other groups in the treatment group (p<0.001). Despite being less wound contraction in the treatment group, the process was more than other groups in day 14 (p<0.001). While in the treatment group, the process of wound contraction was less severe at the beginning, it was more strongly than the other groups in the following (p<0.001). Complete wound healing was observed in all groups at day 21. In this study, the application of Typha angustifolia pollen extract had a positive effect on wound healing process in mice and therefore it might be effective in wound healing.
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