Document Type : Research Paper
Authors
1
MSc Student of Histology and Embryology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2
Assistant Professor, Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3
Associated Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4
Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
5
Associated Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract
Cyclophosphamide is a common chemotherapic medicine but it has toxic side effects. Different medicines are used to prevent its side effects. The aim of this study is evaluation of the protective effects of Mesna against cyclophosphamide toxicity in rat placenta tissue.
Fifteen pregnant rats were randomly divided into 3 groups (1) control (normal saline), (2) cyclophosphamide (15 mg/kg), (3) cyclophosphamide (15mg/kg) and Mesna (100mg/kg). Medicines were administered intraperitoneally on the 13th day of pregnancy. Rats were sacrificed on the 20th day. The placentae were separated and floated in fixative solution. Five µm placental tissue sections were prepared and after staining generally and specially they were studied by light microscopy. Cyclophosphamide decreased placental weight and diameters (P<0.05). Histologically, it induced labyrinth and basal layers thickness reduction (P<0.05) their necrosis and congestion, giant cell number reduction (P<0.05) and their nuclear shrinkage. Mesna has no effect on placental morphometry rather than cyclophosphamide (P>0.05) but histologically, increased labyrinth and basal layers thickness and giant cells number (P<0.05). It also prevented nuclear shrinkage and necrosis in giant cells and spongiotrophoblasts in the basal layer and reduce congestion in the labyrinth layer (P<0.05). Mesna sulfhydryl groups possibly react with acrolein, the major factor of cyclophosphamide toxicity, and change it to nontoxic compounds. Mesna may protect rat placental tissue against cyclophosphamide toxic effects by this mechanism.
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