Evaluation of a part of the dietary methionine substitution by betaine on fatty acid synthase gene expression in laying hens under heat stress

Radpoor, Sosan; Beiginasiri, Mohammad Taghi; Roshanfekr, Hedayatolah; Nazari, Mahmood

doi:10.22055/ivj.2017.45989

Evaluation of a part of the dietary methionine substitution by betaine on fatty acid synthase gene expression in laying hens under heat stress

Document Type : Research Paper

Authors

¹ PhD Student of Animal Breeding, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran

² Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran

³ Associate Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran

⁴ Assistant Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran

10.22055/ivj.2017.45989

Abstract

Current study was performed to evaluate a part of the dietary methionine substitution by betaine on fatty acid synthase gene expression in laying hens under heat stress. Present study was conducted in a completely randomized design (CRD) with a 2 ×2 factorial experiment using 96 (65-week) hens in Line strains W36. First factor was done with 2 dietary treatments with 2 levels; 100% methionine, 87% methionine and 13% betaine and second factor with 1 level without heat stress and 1 with (salon with 35 ^oC); (Salon with 23 ^oC) with 4 treatments and 3 repetition (8 birds in each repetition). To achieve appropriate stress, the hens were daily exposed under 35 ^oC for 6 hours. After 2 month of feeding, 4 birds were eventually slaughtered and fatty acid synthase gene expression was assessed by means of specific primer and real time PCR analysis. The results indicated that addition of betaine to died and exposure heat stress caused a significant decline in fatty acid synthase gene expression being 1.98 and 2.15 fold in liver tissue, respectively (P<0.01). Hence, addition of betaine and heat stress exposure might inhibit fatty acid synthase gene expression in mRNA state and reduce fat synthesis in the body by reducing the expression of fatty acid synthase. There for, betaine may adverse the effects of heat stress in poultry.

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