Phytoestrogenic plants have different effects on the reproductive system. Alfalfa (Medicago sativa) is a phytoestrogenic herb which is used in human and animal nutrition. There are many reports about widely effects of alfalfaon reproductive system. The aim of this study was to evaluate the alfalfa effects on histomorphometrical of the rat seminal vesicle. For this purpose, 30 adult male Wistar rats were divided into control and treatment groups and each group were divided into three subgroups (5 rats in each). Control rats were fed only by 140 g concentrate (plate). The treatment groups were fed by 80 g of concentrate (plate) and 300 g alfalfa. In the end of each period (30, 45 and 60 days), the seminal vesicles were removed and examined macroscopically. After that, 5-6 µm sections were made using paraffin embedding method. The sections were stained by H&E and PAS. Seminal vesicle weight in the treatment group 1 and 2 were decreased significantly (P< 0.01 and P< 0.05 respectively) compared to their control groups but it has not seen a significant difference in group 3. The results of histometrical studies showed that the total wall thickness and tunica muscularis of seminal vesicle increased insignificantly in the treatment group 2 and 3, but they increased in the treatment group 1 significantly. Thickness of the epithelium increased in the treatment group 2 and 3 significantly, but there was not a significant difference in the treatment group1. The results showed that, although the alfalfa has some structural effects on macroscopic and microscopic seminal vesicle of male rats in 30, 45 and 60 days periods but these changes are very mild and may have not considerable effect on reproductive performance.
Erfani Majd, N., Teimourpour, S., Mohammadi, G., & Fatemi Tabatabaei, S. R. (2017). The histomorphometrical effects of Alfalfa on rat seminal vesicle. Iranian Veterinary Journal, 12(4), 79-89. doi: 10.22055/ivj.2017.43036
MLA
Naeem Erfani Majd; Samira Teimourpour; Ghodratollah Mohammadi; Seyed Reza Fatemi Tabatabaei. "The histomorphometrical effects of Alfalfa on rat seminal vesicle". Iranian Veterinary Journal, 12, 4, 2017, 79-89. doi: 10.22055/ivj.2017.43036
HARVARD
Erfani Majd, N., Teimourpour, S., Mohammadi, G., Fatemi Tabatabaei, S. R. (2017). 'The histomorphometrical effects of Alfalfa on rat seminal vesicle', Iranian Veterinary Journal, 12(4), pp. 79-89. doi: 10.22055/ivj.2017.43036
VANCOUVER
Erfani Majd, N., Teimourpour, S., Mohammadi, G., Fatemi Tabatabaei, S. R. The histomorphometrical effects of Alfalfa on rat seminal vesicle. Iranian Veterinary Journal, 2017; 12(4): 79-89. doi: 10.22055/ivj.2017.43036
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