The comparison effects of diet supplemented with alphamune and biomin on immune response against killed influenza vaccine subtype H9N2 in broiler chicks

Mayahi, M; Saify Abad Shapouri, M; Pourmehdi Broujeni, M; Behzadi, A

The comparison effects of diet supplemented with alphamune and biomin on immune response against killed influenza vaccine subtype H9N2 in broiler chicks

Document Type : Research Paper

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Abstract

In order to study the effect of alphamune and biomin on the immune response against killed avian influenza vaccine, three hundred day-old chicks were randomly divided into 4 equal groups and each group divided into 3 subgroups. Chicks of group 1 only received basic feed, groups 2 and 3 received basic feed plus alphamune 0.5 kg and biomin 1kg per ton respectively. Group 4 chicks received only basic feed. Groups 2, 3 and 4 vaccinated with killed avian influenza vaccine subtype H₉N₂ at 9 days old subcutaneously on the back of the neck. Blood samples collected from 15 chicks of each group via wing vein, before of vaccination, 20 and 35 days after vaccination. Blood serum samples collected into microtube and kept in freezer up to test. Antibody titer against killed avian influenza vaccine was measured by hemagglutination –inhibition (HI) test with 4 HA unit influenza antigen. The data were analysed with SPSS version 16. The results of present study indicated that, blood serum antibody mean titer against influenza vaccinein group receiving biomin (3.98) was higher than group receiving alphamune (3.85) at 35 days after vaccination but the difference with vaccinated control group was no significance. It was concluded that, adding alphamune and biomin in the feed of broiler chicks could not affect immune response against killed avian influenza vaccine.

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