Investigating Effect of adding Camelia siensies powder to diet of Cyprinus carpio and its effect on immune factors

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Abstract

    In this experiment the effects of Camellia sinensis as a phytobiotic on the immunity factors of the Cyprinus carpio was examined. For this purpose 360 juvenile Cyprinus carpio with the average weight of 22± 1g were selected and after being adapted to the environment the fish were stored in 12 tanks (30 fish in each tank). The Camellia sinensis was added to the diet in three levels 1.5% (treatment 2) 3% (treatment 3) and 5% (treatment 4) and the diet without Camellia sinensis was used for the control group (treatment 1). Each treatment was recurred 3 times a day. The fish were fed 3% of their weight. At the end of the 45 day treatment period, 15 fish were randomly selected from each treatment and their blood sample was taken from the caudal vein. The immunity factors including differential white blood cell count, total protein, immunoglobulin M (IgM) serum, albumin and lysozyme were analyzed and the results showed that the lysozyme activity had Significantly difference in compared with control treatment (P<0.05) and maximum amount of it was observed in 1.5 % treatment that was equal to 135.67±15.33 . Also, maximum of total Protein 4.89±0.48 and maximum of immunoglubin M 3.35±0.52 were in 1.5 % treatment (P<0.05), but albumin amount was alfected by none of measured factors in exprement (P>0.05).  In general that prescribing Camellia sinensis at 1.5% level orally stimulates some non-specific blood and immune factors and it can be used as the immunity stimulant for the juvenile Cyprinus carpio.

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