Infection of broiler chickens to Ornithobacterium rhinotracheale In Urmia city

Document Type : Research Paper

Authors

1 Veterinary Department

2 Assistant professor, Department of clinical sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, iran.

3 Researcher Follow, Department of veterinary, Agricultural and Natural Resources Rresearch Center, Guilan, Iran

Abstract

    Ornithobacterium rhinotracheale (ORT) a bacterium is known to be associated with respiratory disease, growth retardation, mortality and decrease egg production. The aim of the present study was conducted seroprevalence, isolation, and molecular diagnosis of O. rhinotracheale by PCR method. In this study, 355 serum samples were collected from 20 broiler flocks. Serological results showed that 15 flocks (75%) were positive to O. rhinotracheale infection by ELISA. Total of 200 tracheal swabs (20 samples) were collected. The O. rhinotracheale was isolated from tracheal swabs of 5 flocks (25%) by culture and but with using specific primers of ORT in PCR method was detected A 784 bp fragment of the 16SrRNA gene from 8 flocks (40%). By statistical, there was no significantly different between correlation of the isolates and flocks ORT titers (P>0.05). The results of this study indicated that the prevalence of Ornithobacterium rhinotracheale antibodies is high in the broiler flocks in Urmia city and PCR is a suitable method for diagnosis of the O. rhinotracheale

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