Comparison of antimicrobial and antioxidant activities of essential oil and Nano-emulsion of Ferulago angulata (Chavir) collected from the western regions of Iran

Document Type : Research Paper

Authors

1 PhD Graduated, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Associate Professor, Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Professor, Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Associate Professor, Department of Pharmaceutics, Faculty of Pharmacy and Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Abstract

    Natural preservatives are widely used in the food industry, today. In this study, the essential oil of Chavir plant (Ferulago angulate) was formulated as a water-dispersible nano-emulsion (diameter = 42 nm) using high pressure ultrasonic method. Analysis of essential oil composition showed that monoterpene hydrocarbons and oxygen monoterpenes comprised the main compounds of essential oil. The antimicrobial activity of essential oil and Nano-emulsion oil was evaluated with determination of the minimum inhibitory concentration (MIC) and the minimum biocidal concentration (MBC) as well as the disc diffusion agar methods on four pathogenic bacterial strains and two important fungi that cause foodborne illness. In addition, antioxidant activity was measured using DPPH free radical scavenging method and ABTS+ radical cation. The results showed that the antimicrobial activity of the essential oil increases when it is converted to Nano-emulsion due to easier access of Nano-emulsion to the bacterial cell membrane. In general, gram-positive bacteria displayed more susceptibility to the Nano-emulsion than gram-negative bacteria. Listeria monocytogenes was the most susceptible bacteria to the antibacterial activity of essential oils and Nano-emulsion. In addition, essential oils and Nano-emulsion had significant effects on inhibiting the growth of Aspergillus niger and Candida albicans. Although the antioxidant activities of essential oils and Nano-emulsion were significantly lower than the standard group (BHT), the antioxidant activity of Nano-emulsion was higher than in free essential oils using both methods. The results of this study showed that by converting essential oil to the Nano-emulsion of Chavir essential oil, its antimicrobial and antioxidant properties can be increased.

Keywords


Ahmadi, F., Sadeghi, S., Modarresi, M., Abiri, R., & Mikaeli, A. (2010). Chemical composition, in vitro anti-microbial, antifungal and antioxidant activities of the essential oil and methanolic extract of Hymenocrater longiflorus Benth., of Iran. Food and Chemical Toxicology, 48(5), 1137-1144.
Akhlaghi, H. (2012). Volatile Constituents from the aerial parts of Ferulago angulata (Schlecht.) Boiss. growing wild northeast Iran. Analytical Chemistry Letters, 2(2), 133-138.
Artiga-Artigas, M., Acevedo-Fani, A., & Martín-Belloso, O. (2017). Improving the shelf life of low-fat cut cheese using nanoemulsion-based edible coatings containing oregano essential oil and mandarin fiber. Food Control, 76, 1-12.
Azarbani, F. A. R. I. D. E. H., Saki, Z. E. I. N. A. B., Zareei, A., & Mohammadi, A. B. D. O. L. N. A. S. S. E. R. (2014). Phenolic contents, antibacterial and antioxidant activities of flower, leaf and stem extracts of Ferulago angulata (Schlecht) Boiss. International Journal of Pharmacy and Pharmaceutical Sciences, 6(10), 123-125.
Azizkhani, M., Misaghi, A., Basti, A. A., Gandomi, H., & Hosseini, H. (2013). Effects of Zataria multiflora Boiss. essential oil on growth and gene expression of enterotoxins A, C and E in Staphylococcus aureus ATCC 29213. International Journal of Food Microbiology, 163(2-3), 159-165.
Basile, A., Sorbo, S., Spadaro, V., Bruno, M., Maggio, A., Faraone, N., & Rosselli, S. (2009). Antimicrobial and antioxidant activities of coumarins from the roots of Ferulago campestris (Apiaceae). Molecules, 14(3), 939-952.
Biasi-Garbin, R., Saori Otaguiri, E., Morey, A. T., Fernandes da Silva, M., Belotto Morguette, A. E., Armando Contreras Lancheros, C., ... & Yamada-Ogatta, S. F. (2015). Effect of eugenol against Streptococcus agalactiae and synergistic interaction with biologically produced silver nanoparticles. Evidence-Based Complementary and Alternative Medicine, 2015.
Cakir, A., Kordali, S., Kilic, H., & Kaya, E. (2005). Antifungal properties of essential oil and crude extracts of Hypericum linarioides Bosse. Biochemical Systematics and Ecology, 33(3), 245-256.
Chen, J., Gao, D., Yang, L., & Gao, Y. (2013). Effect of microfluidization process on the functional properties of insoluble dietary fiber. Food Research International, 54(2), 1821-1827.
Darderafshi, M. J., Bahrami, G. H., Sadeghi, E., Khanahmadi, M., Mohammadi, M., & Mohammadi, R. (2014). The effect of Ferulago angulata essential oil on Staphylococcus aureus during the manufacture and preservation of Iranian white cheese. Iranian Journal of Nutrition Sciences & Food Technology, 8(4), 13-20.
Dussault, D., Vu, K. D., & Lacroix, M. (2014). In vitro evaluation of antimicrobial activities of various commercial essential oils, oleoresin and pure compounds against food pathogens and application in ham. Meat Science, 96(1), 514-520.
Elizaquível, P., Azizkhani, M., Sánchez, G., & Aznar, R. (2013). Evaluation of Zataria multiflora Boiss. essential oil activity against Escherichia coli O157: H7, Salmonella enterica and Listeria monocytogenes by propidium monoazide quantitative PCR in vegetables. Food Control, 34(2), 770-776.
Ghosh, V., Mukherjee, A., & Chandrasekaran, N. (2013). Ultrasonic emulsification of food-grade nanoemulsion formulation and evaluation of its bactericidal activity. Ultrasonics Sonochemistry, 20(1), 338-344.
Hosseinnia, M., Khaledabad, M. A., & Almasi, H. (2017). Optimization of Ziziphora clinopodiodes essential oil microencapsulation by whey protein isolate and pectin: A comparative study. International Journal of Biological Macromolecules, 101, 958-966.
Kelen, M., & Tepe, B. (2008). Chemical composition, antioxidant and antimicrobial properties of the essential oils of three Salvia species from Turkish flora. Bioresource Technology, 99(10), 4096-4104.
Khanahmadi, M., & Janfeshan, K. (2006). Study on antioxidation property of Ferulago angulata plant. Asian Journal of Plant Sciences.
Luximon-Ramma, A., Bahorun, T., Soobrattee, M. A., & Aruoma, O. I. (2002). Antioxidant activities of phenolic, proanthocyanidin, and flavonoid components in extracts of Cassia fistula. Journal of Agricultural and Food Chemistry, 50(18), 5042-5047.
McClements, D. J. (2012). Nanoemulsions versus microemulsions: terminology, differences, and similarities. Soft Matter, 8(6), 1719-1729.
Moghimi, R., Ghaderi, L., Rafati, H., Aliahmadi, A., & McClements, D. J. (2016). Superior antibacterial activity of nanoemulsion of Thymus daenensis essential oil against E. coli. Food chemistry, 194, 410-415.
Miastkowska, M., Michalczyk, A., Figacz, K., & Sikora, E. (2020). Nanoformulations as a modern form of biofungicide. Journal of Environmental Health Science and Engineering, 18(1), 119-128.
Noori, S., Zeynali, F., & Almasi, H. (2018). Antimicrobial and antioxidant efficiency of nanoemulsion-based edible coating containing ginger (Zingiber officinale) essential oil and its effect on safety and quality attributes of chicken breast fillets. Food Control, 84, 312-320.
Pirbalouti, A., Izadi, A., Malek Poor, F., & Hamedi, B. (2016). Chemical composition, antioxidant and antibacterial activities of essential oils from Ferulago angulata. Pharmaceutical Biology, 54(11), 2515-2520.
Qian, C., & McClements, D. J. (2011). Formation of nanoemulsions stabilized by model food-grade emulsifiers using high-pressure homogenization: Factors affecting particle size. Food Hydrocolloids, 25(5), 1000-1008.
Rivas, L., McDonnell, M. J., Burgess, C. M., O'Brien, M., Navarro-Villa, A., Fanning, S., & Duffy, G. (2010). Inhibition of verocytotoxigenic Escherichia coli in model broth and rumen systems by carvacrol and thymol. International Journal of Food Microbiology, 139(1-2), 70-78.
Ruiz-Navajas, Y., Viuda-Martos, M., Sendra, E., Perez-Alvarez, J. A., & Fernández-López, J. (2012). Chemical characterization and antibacterial activity of Thymus moroderi and Thymus piperella essential oils, two Thymus endemic species from southeast of Spain. Food Control, 27(2), 294-299.
Rustaiyan, A., Sedaghat, S., Larijani, K., Khossravi, M., & Masoudi, S. (2002). Composition of the essential oil of Ferulago angulata (Schlecht.) Boiss. from Iran. Journal of Essential Oil Research, 14(6), 447-448.
Samani, S., Soleimanian-Zad, S., Sheikh-Zeinoddin, M., & Fathi, M. (2019). Evaluation of Zataria multiflora Boiss. and Carum copticum L. Essential Oil Based Nanoemulsions in Inhibition of Byssochlamys fulva Growth in Apple Juice. Journal of Agricultural Science and Technology, 21(2), 357-368.
Shahbazi, Y. (2019). Antioxidant, antibacterial, and antifungal properties of nanoemulsion of clove essential oil. Nanomedicine Research Journal, 4(4), 204-208.
Seibert, J. B., Rodrigues, I. V., Carneiro, S. P., Amparo, T. R., Lanza, J. S., Frézard, F. J. G., ... & Santos, O. D. H. D. (2019). Seasonality study of essential oil from leaves of Cymbopogon densiflorus and nanoemulsion development with antioxidant activity. Flavour and Fragrance Journal, 34(1), 5-14.
Sefidkon, F., & Omidbaigi, R. (2004). Chemical composition of the essential oil of Ferulago angulata from Iran. Journal of Essential Oil Bearing Plants, 7(1), 60-63.
Sepahvand, R., Delfan, B., Ghanbarzadeh, S., Rashidipour, M., Veiskarami, G. H., & Ghasemian-Yadegari, J. (2014). Chemical composition, antioxidant activity and antibacterial effect of essential oil of the aerial parts of Salvia sclareoides. Asian Pacific Journal of Tropical Medicine, 7, S491-S496.
Severino, R., Ferrari, G., Vu, K. D., Donsì, F., Salmieri, S., & Lacroix, M. (2015). Antimicrobial effects of modified chitosan based coating containing nanoemulsion of essential oils, modified atmosphere packaging and gamma irradiation against Escherichia coli O157: H7 and Salmonella Typhimurium on green beans. Food Control, 50, 215-222.
Shahabi, N., Tajik, H., Moradi, M., Forough, M., & Ezati, P. (2017). Physical, antimicrobial and antibiofilm properties of Zataria multiflora Boiss essential oil nanoemulsion. International Journal of Food Science & Technology, 52(7), 1645-1652.
Shahbazi, Y., Karami, N., & Shavisi, N. (2018). Effect of Ziziphora clinopodioides essential oil on shelf life and fate of Listeria monocytogenes and Staphylococcus aureus in refrigerated chicken meatballs. Journal of Food Safety, 38(1), e12394.
Shahbazi, Y., Shavisi, N., Karami, N., & Kakaei, S. (2015). Chemical composition and in vitro antibacterial activity of Ferulago angulata (Schlecht.) Boiss essential oil. Pharmaceutical Sciences, 21(1), 6-11.
Shahbazi, Y., Shavisi, N., Modarresi, M., & Karami, N. (2016). Chemical composition, antibacterial and antioxidant activities of essential oils from the aerial parts of Ferulago angulata (Schlecht.) Boiss and Ferulago bernardii Tomk. & M. Pimen from different parts of Iran. Journal of Essential Oil Bearing Plants, 19(7), 1627-1638.
Shavisi, N., Khanjari, A., Basti, A. A., Misaghi, A., & Shahbazi, Y. (2017). Effect of PLA films containing propolis ethanolic extract, cellulose nanoparticle and Ziziphora clinopodioides essential oil on chemical, microbial and sensory properties of minced beef. Meat Science, 124, 95-104.
Singh, G., Kapoor, I. P. S., Singh, P., de Heluani, C. S., de Lampasona, M. P., & Catalan, C. A. (2008). Chemistry, antioxidant and antimicrobial investigations on essential oil and oleoresins of Zingiber officinale. Food and Chemical Toxicology, 46(10), 3295-3302.
Srinivasan, R. M. J. N., Chandrasekar, M. J. N., Nanjan, M. J., & Suresh, B. (2007). Antioxidant activity of Caesalpinia digyna root. Journal of Ethnopharmacology, 113(2), 284-291.
Tang, S. Y., Manickam, S., Wei, T. K., & Nashiru, B. (2012). Formulation development and optimization of a novel Cremophore EL-based nanoemulsion using ultrasound cavitation. Ultrasonics Sonochemistry, 19(2), 330-345.
Taran, M., Ghasempour, H. R., & Shirinpour, E. (2010). Antimicrobial activity of essential oils of Ferulago angulata subsp. carduchorum. Jundishapur Journal of Microbiology, 3(1), 10-14
Tiwari, B. K., Valdramidis, V. P., O’Donnell, C. P., Muthukumarappan, K., Bourke, P., & Cullen, P. J. (2009). Application of natural antimicrobials for food preservation. Journal of Agricultural and Food Chemistry, 57(14), 5987-6000.
Topuz, O. K., Özvural, E. B., Zhao, Q., Huang, Q., Chikindas, M., & Gölükçü, M. (2016). Physical and antimicrobial properties of anise oil loaded nanoemulsions on the survival of foodborne pathogens. Food Chemistry, 203, 117-123.