Histometrical study of ovary in streptozotocin induced diabetic rats following by nanozinc oxide and zinc oxide administration

Document Type : Research Paper

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Abstract

    An infertility cause in women is diabetes. One of the organs that diabetes has adverse effects on its structure and function is ovary. Nano zinc oxide (nZnO) is the one of zinc derivatives that has been mentioned as a potent anti-diabetic agent in recent studies. Moreover, zinc is an important element in fertility. Thus, at the present study nZnO effects on ovary in diabetic rats has been compared with zinc oxide. Fourty adult female wistar rats were randomly divided into 5 groups as control, sham (normal saline), diabetic (STZ=60 mg/kg, IP), Diabetic receiving ZnO (30 mg/kg) and diabetic receiving nZnO (30 mg/kg). After treatment for 4 weeks, every animal blood glucose and body weight were measured. Following euthanasia, the ovaries were isolated and floated in appropriate  fixative. Five µm sections were prepared as routine histotechnique and stained and studied by light microscopy. Diabetes reduced primordial follicles and corpus luteum numbers (P<0.05) while it had no effect on other follicles including primary, secondary and tertiary follicles and also follicular diameter. ZnO administration prevented reduction of primordial follicles and corpus luteum numbers (P<0.05). nZnO administration prevented reduction of primordial follicles and also increased corpus luteum numbers (P<0.05). In comparison with ZnO, nZnO may have more protective and stimulatory effects on folliculogenesis. 

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