Immunohistochemistry of hepatopancreas of Litopenaeus vannamei, in two different temperature

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Professor, Department of Clinical Sciences and Excellence center of warm water fish health and diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

    This study aimed to investigate the effect of water temperature on the shrimp hepatopancreas of Litopenaeus vannamei by immunohistochemical method. In this research, a total of 50 shrimp with an average length of 23 ± 1 cm and an average weight of 28 ± 0.5 gr were accidentally caught from shrimp farms of the Choebdeh Abadan from Khuzestan province in two different seasons of the year. The shrimp were sampled for the histological and structural study of the hepatopancreas. After killing the shrimp by physical method, the hepatopancreas of each shrimp was transferred to Davidson's solution. The tissue samples were processed by routine histological procedure, using ascending ethanol for dehydration, xylene for clearing, and paraffin impregnation. 4-6 µm sections were taken and stained with hematoxylin-eosin and TUNEL immunohistochemistry technique. Finally, slides were studied by a light microscope equipped with a Dino-lite lens and Dino-capture software. The results indicated that the hepatopancreas structure is a tubular organ that can be affected by water temperature. In the warm season, the diameter of the tubules was smaller than in the cold season (p≤0.05). There was a significant difference in the percentage of apoptosis between the tubule-forming cells in the warm and cold seasons.

Keywords

References

Basir, Z., & Salari-Aliabadi, M. A. (2020). Comparative study of hemocytes of immune system and hepatopancreas of green tiger prawn, Penaeus semisulcatus in cold and warm seasons. Aquatics Physiology and Biotechnology, 7(4), 1-18.
Basir, R., Abdi, R., Kochenin, P., Morevati, H., Peyghan, R., & Basir, Z. (2010). Histomorphology and histopathological changes in hepatopancrease of Litopenaeus vannamei caused by white spot disease. Journal of marine biology, 2(2), 13-18.
Berry, S. E., Simon, C. J., Foote, A. R., Jerry, D. R., & Wade, N. M. (2019). Evaluation of baseline haemolymph biochemistry, volume and total body energetics to determine an accurate condition index in the black tiger shrimp, Penaeus monodon. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 228, 1-9.
Cavalli, R. O., Tamtin, M., Lavens, P., & Sorgeloos, P. (2001). Variations in lipid classes and fatty acid content in tissues of wild Macrobrachium rosenbergii (de Man) females during maturation. Aquaculture, 193(3-4), 311-324.
Chen, Y. H., & He, J. G. (2019). Effects of environmental stress on shrimp innate immunity and white spot syndrome virus infection. Fish & shellfish immunology, 84, 744-755.
Costas, B., Aragão, C., Ruiz-Jarabo, I., Vargas-Chacoff, L., Arjona, F. J., Mancera, J. M., ... & Conceição, L. E. (2012). Different environmental temperatures affect amino acid metabolism in the eurytherm teleost Senegalese sole (Solea senegalensis Kaup, 1858) as indicated by changes in plasma metabolites. Amino acids, 43(1), 327-335.
Cottin, D., Shillito, B., Chertemps, T., Tanguy, A., Léger, N., & Ravaux, J. (2010). Identification of differentially expressed genes in the hydrothermal vent shrimp Rimicaris exoculata exposed to heat stress. Marine Genomics, 3(2), 71-78.
Cuzon, G., Cahu, C., Aldrin, J. F., Messager, J. L., Stephan, G., & Mevel, M. (1980). Starvation effect on metabolism of Penaeus japonicus. In Proceedings of the World Mariculture Society (Vol. 11, No. 1‐4, pp. 410-423). Oxford, UK: Blackwell Publishing Ltd.
Devi, D. P., Hareesh, K., & Reddy, M. S. (2015). Studies on the proximate composition of tropical freshwater prawn Macrobrachium rosenbergii. International Journal of Fisheries and Aquatic Studies, 3(1), 329-336.
Danya, B R.,& Jagadish, N.M. (2014). Growth of cultured white leg shrimp Litopenaeus vannamei (Boone, 1931) of brackish water culture system in summer season with artificial diet,  Advance Applied Science Research, 5: 25-28.
Flegel, T. W. (2012). Historic emergence, impact and current status of shrimp pathogens in Asia. Journal of invertebrate pathology, 110(2), 166-173.
Glick, D., Barth, S., & Macleod, K. F. (2010). Autophagy: cellular and molecular mechanisms. The Journal of pathology, 221(1), 3-12.
Ghasemi, A., Abdi, R., Doraghi, A., Movahedinia, A., Seydi, A. (2014). Effect of Accute Starvation on Histomorphology of Hepatopancreas in White Shrimp (Litopenaeus vannamei). Journal of Cell & Tissue, 5(1), 63-69.
Gholami, A., Abdi, R., Shirali, S., & Basir, Z. (2018). Histophysiology of Head Kidney and Blood Lymphatic System in Acipenser persicus in Cold and Warm Seasons. Journal of Oceanography, 9 (33), 59-65.
Dipper, F. (2021). Arthropods. In The Marine World (pp. 258-283). Princeton University Press.
 Kankuan, W., Wanichanon, C., Titone, R., Engsusophon, A., Sumpownon, C., Suphamungmee, W., Morani, F., Masini, M., Novelli, M., Isidoro, C. & Sobhon, P. (2017). Starvation promotes autophagy-associated maturation of the ovary in the giant freshwater prawn, Macrobrachium rosenbergii. Frontiers in physiology, 8, 300.
Liao, I. C., & Chao, N. H. (1986). Development of prawn culture and its related studies in Taiwan. Presented at the National Conference on Prawn Farming Technology.
Masson, I., Díaz, A. C., & Petriella, A. M. (2012). Effect of salinity changes on the midgut gland of Artemesia longinaris (Decapoda, Penaeidae). Latin American Journal of Aquatic Research, 40(2), 358-366.
Medina-Beltrán, V., Luna-González, A., Fierro-Coronado, J. A., Campa-Córdova, Á. I., Peraza-Gómez, V., del Carmen Flores-Miranda, M., & Rivera, J. N. G. (2012). Echinacea purpurea and Uncaria tomentosa reduce the prevalence of WSSV in witheleg shrimp (Litopenaeus vannamei) cultured under laboratory conditions. Aquaculture, 358, 164-169.
Michiels, M. S., Del Valle, J. C., & Mañanes, A. A. L. (2013). Effect of environmental salinity and dopamine injections on key digestive enzymes in hepatopancreas of the euryhaline crab Cyrtograpsus angulatus (Decapoda: Brachyura: Varunidae). Scientia Marina, 77(1), 129-136.
Moradkhani, A., Abdi, R., Abadi, M. A. S. A., Nabavi, S. M., & Basir, Z. (2020). Quantification and description of gut-associated lymphoid tissue in, shabbout, Arabibarbus grypus (actinopterygii: cypriniformes: cyprinidae), in warm and cold season. Acta Ichthyologica  et Piscatoria, 50(4), 423-432.
Niu, D., Jin, K., Wang, L., Sun, F., & Li, J. (2013). Identification of cathepsin B in the razor clam Sinonovacula constricta and its role in innate immune responses. Developmental & Comparative Immunology, 41(1), 94-99.
Nor Faadila, M. I., Harivaindaran, K. V., Tajul, A. Y., & Yang, R. (2013). Biochemical and texture property changes during molting process of tiger prawn, Penaeus monodon. International Food Research Journal, 20(2), 751-758.
Nunes, E. T., Braga, A. A., & Camargo-Mathias, M. I. (2014). Histochemical study of the hepatopancreas in adult females of the pink-shrimp Farfantepenaeus brasiliensis Latreille, 1817. Acta histochemica, 116(1), 243-251.
Panakorn, S. (2012). Opinion article: more on early mortality syndrome in shrimp. Aqua Culture Asia Pacific, 8(1), 8-10.
Peng, J., Wei, P., Chen, X., Zeng, D., & Chen, X. (2016). Identification of cold responsive genes in Pacific white shrimp (Litopenaeus vannamei) by suppression subtractive hybridization. Gene, 575(2), 667-674.
Pinoni, S. A., Michiels, M. S., & Mananes, A. A. L. (2013). Phenotypic flexibility in response to environmental salinity in the euryhaline crab Neohelice granulata from the mudflat and the saltmarsh of a SW coastal lagoon. Marine biology, 160(10), 2647-2661.
Qiu, L., Chen, M. M., Wan, X. Y., Li, C., Zhang, Q. L., Wang, R. Y., ... & Huang, J. (2017). Characterization of a new member of Iridoviridae, Shrimp hemocyte iridescent virus (SHIV), found in white leg shrimp (Litopenaeus vannamei). Scientific reports, 7(1), 1-13.
Rexi, P., Manoharam, J. J., & Priya, P. (2015). Comparison of the proximate composition of fresh and cooked muscles of some prawns. International Journal of Informative & Futuristic Research, 2(10), 3536-3541.
Rezaian, D. M, Machiani, M., Safari, M. R. (2002). Cytology of hepatopancreas in white shrimp of Indian Gulf, J0urnal Veterinary Research, 56(4): 25-29.
Sánchez-Paz, A., García-Carreño, F., Hernández-López, J., Muhlia-Almazán, A., & Yepiz-Plascencia, G. (2007). Effect of short-term starvation on hepatopancreas and plasma energy reserves of the Pacific white shrimp (Litopenaeus vannamei). Journal of Experimental Marine Biology and Ecology, 340(2), 184-193.
Vogt, G. (1992). Palaemon B-cell reo-like virus: a new virus from the hepatopancreas of the shrimp Palaemon elegans. Journal of Invertebrate Pathology, 59(2), 218-220.
Xu, Z., Regenstein, J. M., Xie, D., Lu, W., Ren, X., Yuan, J., & Mao, L. (2018). The oxidative stress and antioxidant responses of Litopenaeus vannamei to low temperature and air exposure. Fish & Shellfish Immunology, 72, 564-571.
Zilli, L., Schiavone, R., Storelli, C., & Vilella, S. (2007). Analysis of calcium concentration fluctuations in hepatopancreatic R cells of Marsupenaeus japonicus during the molting cycle. The Biological Bulletin, 212(2), 161-168.
Zhao, Q., Pan, L., Ren, Q., Wang, L., & Miao, J. (2016). Effect of salinity on regulation mechanism of neuroendocrine-immunoregulatory network in Litopenaeus vannamei. Fish & shellfish immunology, 49, 396-406.

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