The effect of substitution to DL-methionine with L-methionine and dietary protein levels on Expression Myogenic Genes in Japanese quail

Document Type : Research Paper

Authors

1 MSc Graduated of Animal genetic and Breeding, Faculty of Animal Science and Food Technology, Agricultural science and Natural Resources University of Khuzestan, Mollasani, Iran

2 Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Iran

3 Assistant Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Iran

Abstract

    This research was conducted to investigate the effect of different levels of protein (20 and 24%) and the replacement of methionine DL with L methionine on the expression of myogenic genes (atrogin-1 and MYF-5) using the RT-qPCR technique in Japanese quail. This experiment was done in the form of a 2×2 factorial with 4 treatments and 4 repetitions and 15 quail in each replicate. The first treatment included DL-methionine and 20% protein (control group). The second treatment consisted of L methionine and 24% proteins, and the third treatment included L methionine and protein 20% and the fourth treatment included DL-methionine and protein 24%. After 35 days of feeding and keeping the quails, with 8 hours interval of hunger, 2 quails were slaughtered in each replicate, and a piece of their chest has been removed immediately and was transferred to the laboratory with Liquid nitrogen, and froze in -80°c. After extraction of the whole RNA, its quality was measured and was used to generate and synthesis the cDNA. Eventually, the expression of myogenic genes was measured by the real-time PCR method. In this method, 𝜷-actin gene, as the source gene, was used to normalize the data. The results showed that by decreasing the protein level from 24% to 20%, atrogin-1 gene expression increased and the MYF-5 gene expression decreased. Also, the replacement of methionine DL with L-methionine did not have a significant effect on the expression of myogenic genes. The results indicated that DL-methionine could be replaced with L methionine, and a 24% protein level is more suitable than 20% in the Japanese quail diet.

Keywords

References

Aghaei, A., Khosravinia, H., Mamoei, M., Azarfar, A., Shahriari, A., and Ghorbanpor, M. (2018). Effects supplementation of zinc and Vit E on antioxidant enzyme, sexual hormone and some biochemical parameters in breeder flock of Japanese Quails. Iranian Veterinary Journal, 4 (2), 14-24. (In Persian)
Dersjant‐Li, Y. and Peisker, M. (2011). A Review on Recent Findings on Amino Acids Requirements in Poultry Studies. Iranian Journal of Applied Animal Science, 1(2), 73-79.
Dilger, R.N., Kobler, C., Weckbecker, C., Hoehler, D. and Baker, D.H. (2007). 2-keto-4-(Methylthio) butyric acid (Keto analog of methionine) is a safe and efficacious precursor of L-Methionine in chicks. Journal of Nutrition, 137, 1868-1873.
Evonik Industries, AG Health and & Nutrition (2015). Nutritional value of L-Methionine is comparable to DL-Methionine in growing broilers from 14 to 27 days of age. Facts & Figures, 15113.
Hyankova, L., Dedkova, L., Knizetva, H. and Klecner, D. (1997). Responses in growth, food intake and food conversion efficiency to different dietary protein concentrations in meat-type lines of Japanese quail. British Poultry Science, 38, 564-570.
Huyghebaert, G. (1993). Comparison of DL-methionine and methionine hydroxy analog-free acid in broilers by using multi exponential regression models. British Poultry Science, 34, 351–359.
Kaur, S., Mandal, A., Singh, K. and Kadam, M. (2008). The response of Japanese quails (heavy body weight line) to dietary energy levels and graded essential amino acid levels on growth performance and immunocompetence. Livestock Science, 117, 255-262.
Lee, J., Giordano, S. and Zhang, J. (2012). Autophagy, mitochondria and oxidative stress: cross-talk and redox signaling. Journal of Biochemistry, 441, 523–540.
Nasiri Moghadam, H., Hesabi Nameghi, A. and Madayeni, M.M. (2007). Effect of supplementation of methionine and lysine amino acids on yield and carcass characteristics of broiler chicks. Journal Iranian Agriculture, 20, 183-192. (In Persian)
National Research Council. (1994). Nutrition of Poultry. National Academy Press. Washington D. C., U.S.A.
Parvin, R., Mandal, A.B., Singh, S.M. and Thakur, R. (2010). Effect of dietary level of methionine on growth performance and immune response in Japanese quails (Coturnix coturnix japonica). Journal Science Food Agriculture, 90, 471-481.
Pfaffl, M.W., Horgan, G.W. and Dempfle, L. (2002). Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research, 30, pp.1-10.
Ribeiro, A., Dahlke, F. and Kessler, A.M. (2005). Methionine sources do not affect performance and carcass yield of broilers fed vegetable diets and submitted to cyclic heat stress. Brazilian Journal of Poultry Science, 7(3), 159-164.
Sabourin, L.A. and Rudnicki, M.A. (2000). The molecular regulation of myogenesis. Clinical Genetics, 57(1), 16-25.
SAS Institute, (1999). SAS/STAT Users Guide. SAS Inc, NC.
Shen, Y.B., Ferket, P., Park, I., Malheiros, R.D. and Kim, S.W. (2015). Effects of feed grade l-methionine on intestinal redox status, intestinal development, and growth performance of young chickens compared with conventional dl-methionine. Journal of Animal Science, 93, 2977-2986.
Shen, Y.B., Weaver, A.C. and Kim, S.W. (2014). Effect of feed grade L-Methionine on growth performance and gut health in nursery pigs compared to conventional DL-Methionine. Journal of Animal Science, 92, 5530-5539.
Vesco, A.P., Gasparino, E., Oliveira Neto, A.R., Guimarães, S.E. and Marcato, S.M. (2015). Effects of Methionine Supplementation on the Expression of Protein Deposition-Related Genes in Acute Heat Stress-Exposed Broilers. PLoS One, 10(2), e0115821.
Wen, C., Chen, X., Chen, G.Y., Wu, P., Chen, Y.P., Zhou, Y.M. and Wong, T. (2014). Methionine improves breast muscle growth and alters myogenic gene expression in broilers. Journal of Animal Science 92(3), 1068-1073.

Files

Share

How to cite

Statistics