Genetic Diversity of Hottentotta Zagrosensis and H. saulcyi (Scorpions: Buthidae) using RAMS (Random Amplified Microsatellites) in Khuzestan

Document Type : Research Paper

Authors

1 PhD Graduated of Biochemistry, Faculty of Veterinary Medicine at the Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Associate Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Razi Vaccines and Serum Research Institute, Agricultural Research Education and Extension Organization, Ahvaz, Iran

Abstract

    Scorpions belong to the Buthidae family have several genera in the World.  One of the genuses is Hottentotta which is located in Iran. There is no phylogenetic data regarding to this scorpion, in spite of its medical importance. Ten scorpion samples of Hottentotta Zagrosensis and H. saulcyi were collected from different regions of Khuzestan province in Iran. The molecular phylogenetic analysis was carried out using RAMS (Random Amplified Microsatellites) and PCR-RFLP. Of the 6 RAMS primers, that were checked, P-CCA generated 78 sufficiently clear and reproducible bands. The band sizes from 20 scorpion samples ranged from 200-1000 bp. The percentages of the polymorphic and monomorphic bands are 94.8 and 5.2%, respectively. After band score calculations, the similarity level was measured using the Dice coefficient, and denderogram were obtained by the UPGMA algorithms.  The results show that the scorpions within the Hottentotta genus has been grouped in two species H. zagrosensis and H. saulcyi with mean percentage of shared bands 65% and 60%, respectively. Two scorpion samples from Dezful (HZ9 and HS5) are significantly dissimilar within their groups. This result was confirmed by PCR-EFLP. Denderogram results for scorpions in Hottentotta genus located in the Khuzestan showed intraspecies genetic diversity. This study shows RAMS primers could be useful tools to assess genetic diversity in Huttentotta scorpions in Khuzestan.

Keywords

Main Subjects

References

Altschul, S.F.; Gish, W.; Miller, W.; Myers, E.W. and Lipman, D.J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3): 403-410.
Dehghani, R.; Motevali Haghi, F.; Mogaddam, M.; Sedaghat, M.M. and Hajat, H. (2016). Review study of scorpion classification in Iran. Journal of Entomology and Zoology Studies, (4): 440-444. 
Dice, L.R. (1945). Measures of the amount of ecologic association between species. Ecology, 26(3): 297-302.
Edgar, R.C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res, 32: 1792-1797.
Fet, V.; Gantenbein, B.; Gromov, A.V.; Lowe, G. and Lourenco, W.R. (2003). The first molecular phylogeny of Buthidae (Scorpiones). Euscorpius, 4: 1-10.
Gantenbein, B; and Largiader, C.R. (2003). The phylogeographic importance of the Strait of Gibraltar as a gene flow barrier in terrestrial arthropods: a case study with the scorpion Buthus occitanus as model organism. Molecular Phylogenetics and Evolution, 28: 119-130.
Hantula, J.; Dusabenyagasaniy, M.; and Hamelin, R.C. (1996). Random amplified microsatellites (RAMS) - a novel method for characterizing genetic variation within fungi. Eur. J. For. Path, 26: 159-166.
Jafari, H.: Saalabi, F.; Jelodar, A.; Navidpour, S.H.; Jahanifard, E. and Forouzan, A. (2017). Phylogenetic study on Orthochirus iranus by using morphological and molecular methods (Scorpiones: Buthidae). Journal of Entomology and Zoology Studies, 6(3): 304-309.
Jolodar, A. (2019). Molecular Characterization and Phylogeny Analysis Based on Sequences of Cytochrome Oxidase gene From Hemiscorpius lepturus of Iran.  Iran J. Vet. Med, 13 (1): 59-67.
Jolodar, A.; Masoudi, A.R.; Mehrzadi, S. and Davoudi, A. (2013). A. Molecular identification of peptide toxin MeBTX from the Iranian scorpion Mesobuthus eupeus. Iranian Journal of Veterinary, 9 (2): 24-33.
Kovarik, F. (2007). A revision of the genus Hottentotta Birula, 1908, with descriptions of four new species (Scorpiones, Buthidae). Euscorpius, 58: 1-107.
Lamoral, B.H. (1979). The scorpions of Namibia. Annals of the Natal Museum, 23: 497-784.
Mirshamsi, O.; Sari, A.; Elahi, E. and Hosseinie, S. (2010). Phylogenetic relationships of Mesobuthus eupeus (C.L. (Koch, 1839) inferred from COI sequences (Scorpiones: Buthidae). The Journal of Natural History, 44: 2851.
Mirshamsi, O. (2011). Mesobuthus eupeus (Scorpiones: Buthidae) from Iran: A polytypic species complex. Zootaxa, 2929: 1-21.
Nejati, J., Mozafari, E.; Saghafipour, A. and Kiyani, M. (2014). Scorpion fauna and epidemiological aspects of scorpionism in southeastern Iran. Asian Pac J Trop Biomed, 4(1): S217-S221.
Nikkhah, N.; Jolodar, A. and Taghavi Moghadam, A. (2018). Phylogenetic analysis of cytochrome oxidase subunit 1 from the Mesobuthus eupeus (Scorpions: Buthidae) of Khuzestan province. Iranian Veterinary Journal, 14: 102-111.
Oliveira, R.P.; Macedo, A.M.; Chiari, E. and Pena S.D.J. (1997). An alternative approach to evaluating the intraspecific genetic variability of parasites. Parasitol Today, 5:196-200.
Ozkan, O.; Adiguzel, S.; Kar, S.; Kurt, M.; Yakistiran, S.; Cesaretli, Y.; Orman, M. and Karaer, K.Z. (2007). Effects Of Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) venom on rats: correlation among acetyl cholinesterase activities and electrolytes levels. Journal of Venomous Animals and Toxins including Tropical Diseases, 13(1): 69–81.
Sneath, P.H.A. and. Sokal, R.R. (1973). Numerical taxonomy. W.H. Freeman and Company, San Francisco.
Sousa, P.; Froufe, E.; Alves, P.C. and Harris; D.J. (2011). Genetic diversity within scorpions of the genus Buthus from the Iberian Peninsula: mitochondrial DNA sequence data indicate additional distinct cryptic lineages. Journal of Arachnology, 38 (2): 206-211.
Sousa, P.; Froufe, E.; Harris, D.J.; Alves, P.C. and Van der Meijden, A.  (2011). Genetic diversity of Maghrebian Hottentotta (Scorpiones: Buthidae) scorpions based on CO1: new insights on the genus phylogeny and distribution. African Invertebrates, 52 (1): 135-143.
Vachon, M. (1974). Etude des caracteres utilises pour classer les familles et les genres de Scorpions (Arachnides). 1. La trichobothriotaxie en arachnologie. Sigles trichobothriaux et types de trichobothriotaxie chez les scorpions. Bulletin du Museum National dHistoire Naturelle, 3: 857-958.
Weber, J.L. (1990). Informativeness of human (dC-dA)n (dG-dT)n polymorphisms. Genomics, 7: 524-530.
Wu, K,S,; Jones, R.; Danneberger, L. and Scolnik, P.A. (1994). Detection of microsatellite polymorphisms without cloning. Nucleic Acids Res, 22: 3257-3258.
Zietkiewicz, E.,; Rafaeski, A. and Labuda, D. (1994). Genome Fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20: 176-183.
Iranian Veterinary Journal
Volume 17, Issue 2 - Serial Number 71
September 2021
Pages 38-50

Files

Share

How to cite

Statistics