Comparison of infectious laryngotracheitis virus pathogenesis in Bovans White and LSL strains pullets by intratracheal pathogenicity and histopathology indices methods

Document Type : Research Paper

Authors

1 Assistant Professor, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

2 Expert, Buein Zahra Farm, Seamorgh, Qazvin, Iran

3 Associated Professor, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

Abstract

    Infectious laryngotracheitis (ILT) is a highly contagious viral respiratory disease of chickens. The objective of this study was to evaluate the pathogenicity indices of ILT virus in Bovans White and Lohmann Selected Leghorn (LSL) strains. Following isolation, identification and confirming the virus through molecular and serological tests, the viral titer was calculated by Speaman-karber method. The ILT virus (> 103 EID50) was inoculated to thirty 8-week-old pullets of each strain via the intratracheal route. The birds were observed for the clinical signs of ILT up to eight days. There was no significant difference regarding clinical signs, intratracheal pathogenicity index (ITPI) and histopathology index (HPI) between Bovans and LSL stains, respectively. The results of this study indicated that the ILT virus had relatively similar pathogenicity in both strains, however, Bovanse strain may be seen as more sensitive to ILTV than LSL strain due to the higher ITPI and HPI.

Keywords


Alexander, H. S., & Nagy, E. (1997). Polymerase chain reaction to detect infectious laryngotracheitis virus in conjunctival swabs from experimentally infected chickens. Avian diseases 41(3): 646-653.
Boodhoo, N., Gurung, A., Sharif, S., &  Behboudi, S. (2016). Marek’s disease in chickens: a review with focus on immunology. Veterinary Research 47(1): 119.
Coppo, M. J., Hartley, C. A., & Devlin, J. M. (2013). Immune responses to infectious laryngotracheitis virus. Developmental and Comparative Immunology 41(3): 454-462.
Ebrahimi, M., Pourbakhsh, S., Shahsavandi, S., Momayez, R., & Gholami, M. (2003). Isolation and identification of infectious laryngotracheitis virus from commercial flocks of Iran using various techniques. Archives of Razi Institute 56(1): 11-22.
Ebrahimi, M., Shahsavandi, S., & Gholami, M. (2001). Outbreak of infectious laryngotracheitis following vaccination in pullet flock. Archives of Razi Institute 52(1): 19-26.
Ebrahimi, M. M., & Shahsavandi, S. (2008). Evaluation of antibody levels during simultaneous aflatoxicosis and vaccination against infectious laryngotracheitis in pullets. Biologicals 36(5): 327-329.
Fahey, K., York, J., & Bagust, T. (1984). Laryngotracheitis herpesvirus infection in the chicken. II. The adoptive transfer of resistance with immune spleen cells. Avian Pathology 13(2): 265-275.
Fedde, M. (1998). Relationship of structure and function of the avian respiratory system to disease susceptibility. Poultry Science 77(8): 1130-1138.
Garcia, M., Spatz, S., & Guy, J. S. (2013). Infectious laryngotracheitis. In: D. E. Swayne, Diseases of poultry (13th ed., pp: 161-179). New Jersey, US: John Wiley & Sons.
Guy, J.S., Barnes, H.J., & Morgan, L.M. (1990). Virulence of infectious laryngotracheitis viruses: comparison of modified-live vaccine viruses and North Carolina field isolates. Avian Diseases 34(1): 106-113.
Guy, J. S., Barnes, H. J., & Smith, L. (1991). Increased virulence of modified-live infectious laryngotracheitis vaccine virus following bird-to-bird passage. Avian diseases 35(2): 348-355.
Jordan, F. T. W. (1993). Infectious laryngotracheitis. In: J. B. McFerran, & M.S. McNulty, Virus Infection of Bird (1 st edn., pp. 19-35). Amsterdam, The Netherlands: Elsevier Science Publishers B.V.
Jovanovic, S., Savic, M., & Zivkovic, D. (2009). Genetic variation in disease resistance among farm animals. Biotechnology in Animal Husbandry 25(5): 339-347.
Lee, J. Y., Song, J. J., Wooming, A., Li, X., Zhou, H., Bottje, W. G., et al. (2010). Transcriptional profiling of host gene expression in chicken embryo lung cells infected with laryngotracheitis virus. BMC Genomics 11(1): 1-15.
Loudovaris, T., Yoo, B., & Fahey, K. (1991). Genetic resistance to infectious laryngotracheitis in inbred lines of White Leghorn chickens. Avian Pathology 20(2): 357-361.
Oldoni, I., Rodriguez-Avila, A., Riblet, S.M., Zavala, G., & Garcia, M. (2009). Pathogenicity and growth characteristics of selected infectious laryngotracheitis virus strains from the United States. Avian Pathology 38(1): 47-53.
Ou, S. C., & Giambrone, J. J. (2012). Infectious laryngotracheitis virus in chickens. World Journal of Virology 1(5): 142-149.
Poulsen, D.J., Thureen, D.R., & Keeler Jr, C.L. (1998). Comparison of disease susceptibility and resistance in three lines of chickens experimentally infected with infectious laryngotracheitis virus. Poultry Science 77(1): 17-21.
Shahsavandi, S., Jamshidi-Navroud, Z., Firouzi, M., & Ebrahimi, M. M. (2017). Examining responses of chicken embryonic neural stem cell to infectious laryngotracheitis virus infections. Comparative Clinical Pathology 26(2): 493-498.
Stear, M., Bishop, S., Mallard, B., & Raadsma, H. (2001). The sustainability, feasibility and desirability of breeding livestock for disease resistance. Research in Veterinary Science 71(1): 1-7.
Swayne, D. E. (2008). A laboratory manual for the isolation and identification of avian pathogens (4th ed). International Book distributing Co. in association American Association of Avian Pathologists, University of Pennsylvania, US. Pp: 94-98.
Tsai, H., Saif, Y., Nestor, K., Emmerson, D., & Patterson, R. (1992). Genetic variation in resistance of turkeys to experimental infection with Newcastle disease virus. Avian Diseases 36(3): 561-565.
Van Dorp, T., Dekkers, J., Martin, S., & Noordhuizen, J. (1998). Genetic parameters of health disorders, and relationships with 305-day milk yield and conformation traits of registered Holstein cows. Journal of Dairy Science 81(8): 2264-2270.
Wijga, S., Parmentier, H. K., Nieuwland, M. G. B., & Bovenhuis, H. (2009). Genetic parameters for levels of natural antibodies in chicken lines divergently selected for specific antibody response. Poultry Science 88(9): 1805-1810.